CAJ | 학술논문

目的探讨miR-146a含量变化在甲状腺乳头状癌(PTC)发生发展过程中的作用及意义.方法应用荧光定量聚合酶链反应(polymerase chain reaction,PCR)检测43例PTC、36例结节性甲状腺肿组织的miR-146a含量变化及32例PTC、23例结节性甲状腺肿患者外周血的miR-146a含量变化,并根据PTC患者主要临床病理参数进行分析.结果 ①PTC与结节性甲状腺肿组织中miR-146a相对含量分别为0.0280±0.0131和0.0212±0.0111,差异有统计学意义(P＜0.05)；PTC组织的miR-146a含量变化与患者淋巴结转移、肿瘤分期等临床病理参数有明显关系(P＜0.05),与患者年龄、肿瘤大小等无关(P＞0.05).②PTC与结节性甲状腺肿外周血中miR-146a相对含量分别为0.0891±0.0419和0.0922±0.0456,2者差异无统计学意义(P＞0.05)；PTC外周血的miR-146a含量变化与患者年龄、肿瘤大小、淋巴结转移、肿瘤分期等无相关性(P＞0.05).结论 miR-146a在PTC中表达升高,与淋巴结转移、肿瘤分期等临床病理参数有明显关系,多见于进展期,提示miR-146a在PTC的发生发展过程中起一定作用.
목적 탐토miR-146a함량변화재갑상선유두상암(PTC)발생발전과정중적작용급의의.방법 응용형광정량취합매련반응(polymerase chain reaction,PCR)검측43례PTC、36례결절성갑상선종조직적miR-146a함량변화급32례PTC、23례결절성갑상선종환자외주혈적miR-146a함량변화,병근거PTC환자주요림상병리삼수진행분석.결과 ①PTC여결절성갑상선종조직중miR-146a상대함량분별위0.0280±0.0131화0.0212±0.0111,차이유통계학의의(P＜0.05)；PTC조직적miR-146a함량변화여환자림파결전이、종류분기등림상병리삼수유명현관계(P＜0.05),여환자년령、종류대소등무관(P＞0.05).②PTC여결절성갑상선종외주혈중miR-146a상대함량분별위0.0891±0.0419화0.0922±0.0456,2자차이무통계학의의(P＞0.05)；PTC외주혈적miR-146a함량변화여환자년령、종류대소、림파결전이、종류분기등무상관성(P＞0.05).결론 miR-146a재PTC중표체승고,여림파결전이、종류분기등림상병리삼수유명현관계,다견우진전기,제시miR-146a재PTC적발생발전과정중기일정작용.
Objective To study the change of miR-146a in the development and progression of papillary thyroid carcinoma(PTC).Methods Real-time fluorescence quantitative polymerase chain reaction was used to detect the change of miR-146a in tumor tissues of 43 cases of PTC and 36 cases of nodular goiter,also in peripheral blood of 32 cases of PTC and 23 cases of nodular goiter.The correlation of miR-146a change and the clinicopathological parameters of patients with PTC was analyzed.Results ① The relative content of miR-146a in the 43 patients with PTC and 36 patients with nodular goiter was 0.0280 ± 0.0131 and 0.0212 ± 0.0111 respectively.The difference had statistical significance (P ＜ 0.05).miR-146a in PTC tissues was significantly correlated to clinicopathological parameters such as lymph node metastasis,tumor stage (P ＜ 0.05),and it was unrelated to age or tumor size(P ＞0.05).② The relative content of miR-146a in peripheral blood of 32 patients with PTC and 23 patients with nodular goiter was 0.0891 ±0.0419 and 0.0922 ±0.0456 respectively.The difference had no statistical significance(P ＞ 0.05).There was no significant correlation between miR-146a in peripheral blood of patients with PTC and age,tumor stage,lymph node metastasis,or tumor size of patients (P ＞ 0.05).Conclusions MiR-146a expression is upregulated in patients with PTC,and there is a significant correlation between miR-146a content and clinicopathological parameters such as lymph node metastasis,tumor stage,especially in advanced stage.It suggests that miR-146a may play a role in carcinogenesis and development of PTC.