CAJ | 학술논문

目的探讨缺氧诱导因子-1α(hypoxia-inducible factor 1α,HIF-1α)和肿瘤细胞增殖抗原Ki67及血管内皮生长因子(VEGF)在乳腺癌组织中表达的临床意义.方法采用免疫组化法检测70例乳腺癌组织及其癌旁组织、10例乳腺正常组织中HIF-1α、Ki67和VEGF的表达情况.采用x2检验分析其阳性率的表达差异,无序分类资料的关联性分析检查3者的相关性.结果乳腺癌组织中HIF-1 α、Ki67和VEGF的阳性表达率分别为78.6％ (55/70)、88.6％ (62/70)和65.7％ (46/70),3者的表达显著高于癌旁及正常组织(P＜0.01)；且与临床分期、淋巴结转移情况密切相关(P＜0.05)；HIF-1 α与Ki67、VEGF的表达强度均呈正相关(P＜0.05).结论 HW-1α、Ki67、VEGF的检测可能成为预测乳腺癌发生发展、侵袭转移和预后的重要指标；组织缺氧可促进乳腺癌细胞增殖及血管生成,HIF-1α可望成为乳腺癌治疗的一个新靶点,值得进一步研究.
목적 탐토결양유도인자-1α(hypoxia-inducible factor 1α,HIF-1α)화종류세포증식항원Ki67급혈관내피생장인자(VEGF)재유선암조직중표체적림상의의.방법 채용면역조화법검측70례유선암조직급기암방조직、10례유선정상조직중HIF-1α、Ki67화VEGF적표체정황.채용x2검험분석기양성솔적표체차이,무서분류자료적관련성분석검사3자적상관성.결과 유선암조직중HIF-1 α、Ki67화VEGF적양성표체솔분별위78.6％ (55/70)、88.6％ (62/70)화65.7％ (46/70),3자적표체현저고우암방급정상조직(P＜0.01)；차여림상분기、림파결전이정황밀절상관(P＜0.05)；HIF-1 α여Ki67、VEGF적표체강도균정정상관(P＜0.05).결론 HW-1α、Ki67、VEGF적검측가능성위예측유선암발생발전、침습전이화예후적중요지표；조직결양가촉진유선암세포증식급혈관생성,HIF-1α가망성위유선암치료적일개신파점,치득진일보연구.
Objective To examine the expression of hypoxia inducible factor-1 α (hypoxia-inducible factor 1α,HIF-1α),tumor cell proliferation antigen Ki67 and vascular endothelial growth factor (VEGF) in breast cancer tissues,adjacent tissues and normal breast tissues.Methods Immunohistochemistry HRP method was used to detect the expression of HIF-1α,Ki67 and VEGF in 70 cases of breast cancer tissues and their adjacent tissues and 10 cases of normal breast tissues.Difference of the positive rate was assessed viax2 test,and the correlation between the 3 were analyzed in a disorderly classified manner.Results The positive expression rate of HIF-1α,Ki67 and VEGF in 70 cases of breast cancer tissues was 78.6％ (55/70),88.6％ (62/70) and 65.7％ (46/70) respectively,significantly higher than that in adjacent tissues and normal breast tissues (P ＜ 0.01).The positive expression rate of HIF-1 α,Ki67 and VEGF was closely related with clinical stage,lymph node metastasis and histological grade (P ＜0.05).The expression intensity of HIF-1α was positively correlated with Ki67 and VEGF in breast cancer (P ＜ 0.05).Conclusions Detection of HIF-1 α,Ki67,VEGF may be an important indicator to predict the development,invasion,metastasis and prognosis of breast cancer.Tissue hypoxia may promote the proliferation of breast cancer cell and angiogenesis.A presumption is that HIF-1αmay be a new t()get of breast cancer,thus it deserves further study.