中华内分泌外科杂志
中華內分泌外科雜誌
중화내분비외과잡지
CHINESE JOURNAL OF ENDOCRINE SURGERY
2013年
3期
218-220
,共3页
王奇龙%孙学军%王林古%张仕运%成亮
王奇龍%孫學軍%王林古%張仕運%成亮
왕기룡%손학군%왕림고%장사운%성량
甲状腺癌%BP1基因%原位杂交%免疫组化
甲狀腺癌%BP1基因%原位雜交%免疫組化
갑상선암%BP1기인%원위잡교%면역조화
Thyroid cancer%BP1 gene%In situ hybridization%Immunohistochemistry
目的 检测BP1在甲状腺癌组织中的表达,探讨其与甲状腺癌临床病理因素的关系.方法 运用原位杂交及免疫组化方法检测60例甲状腺癌组织(研究组)和20例正常甲状腺组织(对照组)中的BP1表达.结果 原位杂交方法检测BP1 mRNA阳性率在研究组中为78.3％ (47/60),对照组中为20％(4/20),BP1基因在2组的表达差异有统计学意义(x2=22.09,P＜0.05);甲状腺癌各病理类型的阳性表达率分别为乳头状癌81.7％(40/49)、滤泡状癌85.7％ (6/7)、髓样癌25.0％(1/4);髓样癌分别与乳头状癌、滤泡状癌之间BP1 mRNA的表达差异有统计学意义(x2=4.21,P＜0.05);免疫组化检测研究组中BP1蛋白阳性率为93.3％(56/60),对照组中BP1蛋白阳性率为10.0％(2/20),2组间BP1的表达差异有统计学意义(x2 =52.25,P＜0.05).国际抗癌联盟(UICC)Ⅰ～Ⅱ期BP1 mRNA阳性率90.9％ (40/44),Ⅲ～Ⅳ期43.8％(7/16),差异有统计学意义(x2=12.72,P＜0.05).但BP1 mRNA表达与性别、年龄及淋巴结转移无相关性.结论 BP1在甲状腺癌组织中的表达显著上调;BP1基因的表达与其UICC分期、病理学类型有关,而与年龄、性别及淋巴转移无关.
目的 檢測BP1在甲狀腺癌組織中的錶達,探討其與甲狀腺癌臨床病理因素的關繫.方法 運用原位雜交及免疫組化方法檢測60例甲狀腺癌組織(研究組)和20例正常甲狀腺組織(對照組)中的BP1錶達.結果 原位雜交方法檢測BP1 mRNA暘性率在研究組中為78.3％ (47/60),對照組中為20％(4/20),BP1基因在2組的錶達差異有統計學意義(x2=22.09,P＜0.05);甲狀腺癌各病理類型的暘性錶達率分彆為乳頭狀癌81.7％(40/49)、濾泡狀癌85.7％ (6/7)、髓樣癌25.0％(1/4);髓樣癌分彆與乳頭狀癌、濾泡狀癌之間BP1 mRNA的錶達差異有統計學意義(x2=4.21,P＜0.05);免疫組化檢測研究組中BP1蛋白暘性率為93.3％(56/60),對照組中BP1蛋白暘性率為10.0％(2/20),2組間BP1的錶達差異有統計學意義(x2 =52.25,P＜0.05).國際抗癌聯盟(UICC)Ⅰ～Ⅱ期BP1 mRNA暘性率90.9％ (40/44),Ⅲ～Ⅳ期43.8％(7/16),差異有統計學意義(x2=12.72,P＜0.05).但BP1 mRNA錶達與性彆、年齡及淋巴結轉移無相關性.結論 BP1在甲狀腺癌組織中的錶達顯著上調;BP1基因的錶達與其UICC分期、病理學類型有關,而與年齡、性彆及淋巴轉移無關.
목적 검측BP1재갑상선암조직중적표체,탐토기여갑상선암림상병리인소적관계.방법 운용원위잡교급면역조화방법검측60례갑상선암조직(연구조)화20례정상갑상선조직(대조조)중적BP1표체.결과 원위잡교방법검측BP1 mRNA양성솔재연구조중위78.3％ (47/60),대조조중위20％(4/20),BP1기인재2조적표체차이유통계학의의(x2=22.09,P＜0.05);갑상선암각병리류형적양성표체솔분별위유두상암81.7％(40/49)、려포상암85.7％ (6/7)、수양암25.0％(1/4);수양암분별여유두상암、려포상암지간BP1 mRNA적표체차이유통계학의의(x2=4.21,P＜0.05);면역조화검측연구조중BP1단백양성솔위93.3％(56/60),대조조중BP1단백양성솔위10.0％(2/20),2조간BP1적표체차이유통계학의의(x2 =52.25,P＜0.05).국제항암련맹(UICC)Ⅰ～Ⅱ기BP1 mRNA양성솔90.9％ (40/44),Ⅲ～Ⅳ기43.8％(7/16),차이유통계학의의(x2=12.72,P＜0.05).단BP1 mRNA표체여성별、년령급림파결전이무상관성.결론 BP1재갑상선암조직중적표체현저상조;BP1기인적표체여기UICC분기、병이학류형유관,이여년령、성별급림파전이무관.
Objective To detect the expression of BP1 gene in thyroid cancer and its relationship with clinicopathological features of thyroid cancer.Methods BP1 gene expression in 60 cases of thyroid cancer tissues and 20 cases of normal thyroid tissues were detected by in situ hybridization and immunohistochemistry.Results The positive expression rate of BP1 mRNA was 78.3 ％ (47/60) in the 60 cases of thyroid cancer tissues while it was 20％ (4/20) in the 20 cases of normal thyroid tissues detected by in situ hybridization.The difference had statistical significance (P ＜ 0.05).Of the 3 pathological types of thyroid cancer,the positive expression rate of papillary carcinoma was 81.6％ (40/49),follicular carcinoma 85.7％ (6/7),and medullary carcinoma 25.0％ (1/4).The expression of BP1 mRNA had statistical difference between medullary carcinoma and other pathological types like papillary carcinoma and follicular carcinoma (P ＜ 0.05).The positive expression rate of BP1 protein was 93.3％ (56/60)in the 60 cases of thyroid cancer tissues while it was 10.0％ (2/20) in the 20 cases of normal thyroid tissues detected by immunohistochemistry.The difference had statistical significance(P ＜0.05).Conclusion BP1 gene expression is up-regulated in human thyroid cancer and it is related to tumor stage and pathological type but not related to patients' age,sex or lymph node metastasis.
