中华内分泌外科杂志
中華內分泌外科雜誌
중화내분비외과잡지
CHINESE JOURNAL OF ENDOCRINE SURGERY
2013年
5期
371-375
,共5页
淋巴管密度%微血管密度%预后%乳腺癌
淋巴管密度%微血管密度%預後%乳腺癌
림파관밀도%미혈관밀도%예후%유선암
Lymphatic vessel density%Microvessels density%Prognosis%Breast carcinoma
目的 探讨淋巴管密度和微血管密度在乳腺癌预后中的意义.方法 选择69例乳腺浸润性导管癌标本,应用免疫组织化学SP染色法用D240、CD31分别标记淋巴管和微血管后进行密度计数,对淋巴管密度(lymphatic vessel density,LVD)、微血管密度(microvessel density,MVD)与临床病理特征及患者的预后关系进行统计学分析.结果 肿瘤周围淋巴管密度(peri-lymphatic vessel density,P-LVD)明显高于肿瘤内淋巴管密度(intra-lymphatic vessel density,I-LVD)及对照组淋巴管密度(P＜0.01).I-LVD与患者年龄、肿块大小、组织学分级、淋巴管浸润、淋巴结转移、雌激素受体(estrogen receptor,ER),孕激素受体(progesterone receptor,PR)及人类表皮生长因子受体2(human epidermal growth factor receptor-2,HER2)的表达等临床病理因素无关(P＞0.05),而P-LVD与淋巴结转移,肿瘤的临床分期密切相关(P =0.003、0.026),MVD仅与临床分期密切相关(P=0.038).在生存分析中发现P-LVD、MVD与患者的总生存期、无病生存期密切相关(P =0.007、0.008、0.014、0.024,log-rank检验),多因素分析提示P-LVD、MVD是患者总生存期的独立预后因素.结论 乳腺癌P-LVD、MVD与患者的预后密切相关,通过定量分析,P-LVD、MVD可预测乳腺癌患者的预后.
目的 探討淋巴管密度和微血管密度在乳腺癌預後中的意義.方法 選擇69例乳腺浸潤性導管癌標本,應用免疫組織化學SP染色法用D240、CD31分彆標記淋巴管和微血管後進行密度計數,對淋巴管密度(lymphatic vessel density,LVD)、微血管密度(microvessel density,MVD)與臨床病理特徵及患者的預後關繫進行統計學分析.結果 腫瘤週圍淋巴管密度(peri-lymphatic vessel density,P-LVD)明顯高于腫瘤內淋巴管密度(intra-lymphatic vessel density,I-LVD)及對照組淋巴管密度(P＜0.01).I-LVD與患者年齡、腫塊大小、組織學分級、淋巴管浸潤、淋巴結轉移、雌激素受體(estrogen receptor,ER),孕激素受體(progesterone receptor,PR)及人類錶皮生長因子受體2(human epidermal growth factor receptor-2,HER2)的錶達等臨床病理因素無關(P＞0.05),而P-LVD與淋巴結轉移,腫瘤的臨床分期密切相關(P =0.003、0.026),MVD僅與臨床分期密切相關(P=0.038).在生存分析中髮現P-LVD、MVD與患者的總生存期、無病生存期密切相關(P =0.007、0.008、0.014、0.024,log-rank檢驗),多因素分析提示P-LVD、MVD是患者總生存期的獨立預後因素.結論 乳腺癌P-LVD、MVD與患者的預後密切相關,通過定量分析,P-LVD、MVD可預測乳腺癌患者的預後.
목적 탐토림파관밀도화미혈관밀도재유선암예후중적의의.방법 선택69례유선침윤성도관암표본,응용면역조직화학SP염색법용D240、CD31분별표기림파관화미혈관후진행밀도계수,대림파관밀도(lymphatic vessel density,LVD)、미혈관밀도(microvessel density,MVD)여림상병리특정급환자적예후관계진행통계학분석.결과 종류주위림파관밀도(peri-lymphatic vessel density,P-LVD)명현고우종류내림파관밀도(intra-lymphatic vessel density,I-LVD)급대조조림파관밀도(P＜0.01).I-LVD여환자년령、종괴대소、조직학분급、림파관침윤、림파결전이、자격소수체(estrogen receptor,ER),잉격소수체(progesterone receptor,PR)급인류표피생장인자수체2(human epidermal growth factor receptor-2,HER2)적표체등림상병리인소무관(P＞0.05),이P-LVD여림파결전이,종류적림상분기밀절상관(P =0.003、0.026),MVD부여림상분기밀절상관(P=0.038).재생존분석중발현P-LVD、MVD여환자적총생존기、무병생존기밀절상관(P =0.007、0.008、0.014、0.024,log-rank검험),다인소분석제시P-LVD、MVD시환자총생존기적독립예후인소.결론 유선암P-LVD、MVD여환자적예후밀절상관,통과정량분석,P-LVD、MVD가예측유선암환자적예후.
Objective To investigate tumor lymphatic and mircovascular densities as prognostic markers in 69 cases of invasive breast cancer treated with partial or total mastectomy and lymph node dissection.Methods 69 cases of untreated primary unilateral invasive ductal breast carcinomas were selected.All cases were immunostained with D2-40 and CD31.Positively stained microvessels were counted in densely vascular/lymphatic foci (hot spots).The relationship between lymphatic vessel density (LVD),microvessel density(MVD) and prognosis was analyzed.Results The mean ± SD peritumoral lymphatic vessel density (P-LVD) was significantly higher than intratumoral LVD(I-LVD) (P ＜ 0.01).There was a positive correlation of D2-40 LVD(peritumoral) counts with lymph node metastasis (P =0.003) and clinical stage (P =0.026),and CD31 microvessel density was found significantly associated with clinical stage(P =0.038).No significant association was found between above variants with I-LVD (P ＞ 0.05).Univariate analysis showed that survival time was impaired by higher MVD and higher peritumoral LVD(P =0.007,P =0.008,P =0.014,P =0.024,log-rank test),but not I-LVD.Multivariate survival analysis showed that MVD,peritumoral LVD,TNM stage and lymph node metastasis were independent prognostic factors for overall survival.Conclusions Peritumoral LVD and MVD were significantly correlated with survival status of patients with breast cancer.This is the first attempt to predict prognosis of breast cancer patients by quantifying the peritumoral LVD and MVD.
