CAJ | 학술논문

目的探讨促红细胞生成素产生肝细胞受体A2(EphA2)在儿童脑胶质瘤中的表达水平及其意义.方法收集2009年1月至2013年3月在郑州大学第五附属医院住院的具有明确病理分级的儿童胶质瘤标本78例为胶质瘤组,其中低级别组36例(Ⅰ～Ⅱ级)、高级别组42例(Ⅲ～Ⅳ级),另收集脑外伤行内减压去除的儿童脑组织标本33例为对照组.分别采用反转录(RT)-PCR、Western blot和免疫组织化学法测定胶质瘤组和对照组中EphA2 mRNA及蛋白表达水平.结果 1.RT-PCR和Western blot显示,EphA2 mRNA在对照组脑组织中不表达,而在胶质瘤组中均呈高表达,差异有统计学意义(F=36.271,P＜O.05);EphA2蛋白在高级别儿童胶质瘤组表达水平显著高于低级别儿童胶质瘤组,差异有统计学意义(F=42.839,P ＜0.05).2.免疫组织化学法显示,EphA2在对照组脑组织中呈阴性表达,在低级别胶质瘤组中的阳性表达为88.57％,在高级别胶质瘤组中的阳性表达为100.00％,且胶质瘤的级别越高,EphA2的阳性表达分布越强,差异有统计学意义(Z =4.962,P＜0.05).结论 EphA2在儿童脑胶质瘤中呈高表达,且与胶质瘤的病理分级相关,可能参与儿童脑胶质瘤的发生和进展.
목적 탐토촉홍세포생성소산생간세포수체A2(EphA2)재인동뇌효질류중적표체수평급기의의.방법 수집2009년1월지2013년3월재정주대학제오부속의원주원적구유명학병리분급적인동효질류표본78례위효질류조,기중저급별조36례(Ⅰ～Ⅱ급)、고급별조42례(Ⅲ～Ⅳ급),령수집뇌외상행내감압거제적인동뇌조직표본33례위대조조.분별채용반전록(RT)-PCR、Western blot화면역조직화학법측정효질류조화대조조중EphA2 mRNA급단백표체수평.결과 1.RT-PCR화Western blot현시,EphA2 mRNA재대조조뇌조직중불표체,이재효질류조중균정고표체,차이유통계학의의(F=36.271,P＜O.05);EphA2단백재고급별인동효질류조표체수평현저고우저급별인동효질류조,차이유통계학의의(F=42.839,P ＜0.05).2.면역조직화학법현시,EphA2재대조조뇌조직중정음성표체,재저급별효질류조중적양성표체위88.57％,재고급별효질류조중적양성표체위100.00％,차효질류적급별월고,EphA2적양성표체분포월강,차이유통계학의의(Z =4.962,P＜0.05).결론 EphA2재인동뇌효질류중정고표체,차여효질류적병리분급상관,가능삼여인동뇌효질류적발생화진전.
Objective To investigate the expressions and significance of erythropoietin producing hepatocellular cell line receptor A2 (EphA2) in pediatric brain glioma.Methods Seventy-eight fresh pediatric glioma specimens with a defined histological grade were collected in the Fifth Affiliated Hospital of Zhengzhou University from Jan.2009to Mar.2013,which included 36 of low grade glioma(Ⅰ-Ⅱ grade),42 of high grade glioma(Ⅲ-grade),another 33 cases with brain trauma line pressure to remove children brain tissues were collected as control group.The expressions of EphA2 mRNA and protein were detected by reverse transcription-polymerase chain reaction (RT-PCR),Western blot and immunohistochemistry.Results 1.RT-PCR and Western blot showed that EphA2 did not express in control brain tissue,but the expression levels of EphA2 mRNA were over-expressed in pediatric brain glioma,and the difference was statistically significant(F =36.271,P ＜ 0.05) ;the expression levels of EphA2 protein were significantly higher in high-grade pediatric glioma group than in low-grade pediatric glioma group,and the difference was statistically significant(F =42.839,P ＜ 0.05).2.Immunohistochemistry showed that EphA2 expression was negative in control group,the positive expression in low-grade glioma group was 88.57％,and the positive expression in high-grade glioma group was 100.00％.Moreover,the higher the grade glioma,distribution of EphA2 expression was stronger,and the difference was statistically significant(Z =4.962,P ＜ 0.05).Conclusions The mRNA and protein expression levels of EphA2 were significantly high in pediatric brain glioma which were associated with the grade of glioma.Therefore,EphA2 may participate in the development and progression of pediatric brain glioma.