CAJ | 학술논문

目的探讨复方丹参注射液对感染性休克大鼠腑损伤时一氧化氮(NO)、B-细胞淋巴瘤-2 (bcl-2)蛋白及S100水平的影响.方法选取2周龄健康清洁级Wistar大鼠40只,随机分为4个组,每组10只.感染性休克1组及感染性休克2组:通过尾静脉给予脂多糖(5 mg/kg)复制感染性休克模型;治疗组:造模成功后通过腹腔注射给予复方丹参注射液(5 mg/kg);正常对照组:在其他组给予药物时给予9 g/L盐水替代.除感染性休克2组于造模成功12 h取材外,其余3组均于造模后6h取材.断头法取脑组织及摘眼球法取静脉血,用硝酸还原法检测4组大鼠脑组织中NO水平,免疫组织化学法检测4组大鼠海马组织bcl-2蛋白的表达,酶联免疫吸附法检测4组大鼠静脉血S100水平.结果与正常对照组相比,感染性休克1组及治疗组NO、bcl-2及S100水平均明显升高,差异均有统计学意义(F=25.64、3 027.17、632.04,P均＜0.05);治疗组NO及S100水平均较感染性休克1组明显降低,差异有统计学意义(F=25.64、632.04,P均＜0.05),治疗组bcl-2表达水平较感染性休克1组明显增高,差异有统计学意义(F=3 027.17,P＜0.05).感染性休克2组NO及S100水平较感染性休克1组均升高,差异均有统计学意义(t=-12.34、-4.89,P均＜0.05),感染性休克2组bcl-2表达水平较感染性休克1组降低,差异有统计学意义(t=6.88,P＜0.05).NO与bcl-2呈显著负相关(r=-0.928,P＜0.05),NO与S100呈显著正相关(r=0.877,P＜0.05).结论感染性休克时,NO参与脑损伤的过程,可能与其下调bcl-2的表达,上调S100的表达有关;复方丹参注射液可能通过抑制NO生成,上调bcl-2表达,下调S100表达,对脑组织起保护作用.
목적 탐토복방단삼주사액대감염성휴극대서부손상시일양화담(NO)、B-세포림파류-2 (bcl-2)단백급S100수평적영향.방법 선취2주령건강청길급Wistar대서40지,수궤분위4개조,매조10지.감염성휴극1조급감염성휴극2조:통과미정맥급여지다당(5 mg/kg)복제감염성휴극모형;치료조:조모성공후통과복강주사급여복방단삼주사액(5 mg/kg);정상대조조:재기타조급여약물시급여9 g/L염수체대.제감염성휴극2조우조모성공12 h취재외,기여3조균우조모후6h취재.단두법취뇌조직급적안구법취정맥혈,용초산환원법검측4조대서뇌조직중NO수평,면역조직화학법검측4조대서해마조직bcl-2단백적표체,매련면역흡부법검측4조대서정맥혈S100수평.결과 여정상대조조상비,감염성휴극1조급치료조NO、bcl-2급S100수평균명현승고,차이균유통계학의의(F=25.64、3 027.17、632.04,P균＜0.05);치료조NO급S100수평균교감염성휴극1조명현강저,차이유통계학의의(F=25.64、632.04,P균＜0.05),치료조bcl-2표체수평교감염성휴극1조명현증고,차이유통계학의의(F=3 027.17,P＜0.05).감염성휴극2조NO급S100수평교감염성휴극1조균승고,차이균유통계학의의(t=-12.34、-4.89,P균＜0.05),감염성휴극2조bcl-2표체수평교감염성휴극1조강저,차이유통계학의의(t=6.88,P＜0.05).NO여bcl-2정현저부상관(r=-0.928,P＜0.05),NO여S100정현저정상관(r=0.877,P＜0.05).결론 감염성휴극시,NO삼여뇌손상적과정,가능여기하조bcl-2적표체,상조S100적표체유관;복방단삼주사액가능통과억제NO생성,상조bcl-2표체,하조S100표체,대뇌조직기보호작용.
Objective To explore the inlluence of compound Danshen injection on the expressions of nitric oxide (NO),B-cell lymphoma-2 (bcl-2) protein and S100 in the septic shock rats with brain injury.Methods Forty 2-week-old healthy Wistar rats were randomly divided into 4 groups,10 cases in every group.Septic shock group 1 (6 h) and septic shock group 2 (12 h):the rats were injected with lipopolysaecharide (5 mg/kg) via the tail vein to make the septic shock models;and the treatment group:rats were intraperitoneally injected with compound Danshen injection (5 mg/kg) immediately when shock signs appeared; Normal control group:the rats were given the same amount of 9 g/L saline while the other groups received drugs; The septic shock group 2 were drawn 12 h after the successful mode-ling,while the remaining 3 groups were havested at 6 h after the success of the modeling.The content of NO in the brain tissue was detected with nitrate reduction way,and the expression of bcl-2 in hippocampus was examined with immunohistochemistry techique,meanwhile the concentration of serum S100 was measured by enzyme linked immunosorbent assay.Results Compared with the normal control group,the contents of NO,bcl-2 and S100 in the septic shock 1 group increased significantly (F =25.64,3 027.17,632.04,all P ＜ 0.05) ; however in treatment group,the contents of NO and S100 were less than those in the septic shock groups,while the level of bcl-2 was more than that in the septic shock groups,and there were significant diferences (F =25.64,632.04,3 027.17,all P ＜ 0.05) ; compared with the septic shock group 1,the contents of NO and S100 in the septic shock group 2 increased drastically,but the level of bcl-2 in the septic shock 2 group was decreased,and there were significant differences (t =-12.34,-4.89,6.88,all P ＜ 0.05).NO and bcl-2 had significant negative correlation (r =-0.928,P ＜ 0.05) ; NO had significant positive correlation with S100 (r =0.877,P ＜ 0.05).Conclusions NO participates in brain injury of septic shock rats,through inhibiting bcl-2 expression and up-regulating S100 expression.Compound Danshen injection has a protective effect on the brain tissues of rats during septic shock,through reducing the contents of NO as well as S100 and elevating the expression of bcl-2.