CAJ | 학술논문

目的探讨法舒地尔对缺氧缺血性脑损伤(HIBD)大鼠Nogo髓鞘相关蛋白(Nogo-A)及NF200表达的影响.方法将120只新生7日龄Wistar大鼠按随机数字表法分假手术组(40只)、HIBD组(40只)和法舒地尔治疗组(40只),假手术组只做颈总动脉分离后不结扎,直接缝合切口不做缺氧处理,HIBD组造模后腹腔注射9 g/L盐水,法舒地尔组腹腔注射法舒地尔(10 mg/kg).分别于术后6h、12 h、24 h、72 h和7d断颈处死大鼠.HE染色镜下观察各组大鼠脑组织病理变化.免疫组织化学法检测脑组织Nogo-A和NF200表达.结果 1.肉眼观察:假手术组两侧大脑半球对称;HIBD组随时间延长脑组织水肿加重,可见大脑半球坏死灶;法舒地尔组脑组织水肿减轻.2.HE染色:假手术组脑组织结构形态正常;HIBD组可见细胞肿胀,核固缩、裂解,炎性细胞增多;法舒地尔组神经细胞水肿及核固缩数量减少.3.免疫组织化学:假手术组Nogo-A可见少量表达;HIBD组Nogo-A阳性细胞12h开始升高,24 h达峰值,随后表达逐渐降低;法舒地尔组除6h外,各时间点的表达均低于HIBD组(P ＜0.01);HIBD组和法舒地尔组阳性细胞数多于假手术组(P＜0.01).假手术组NF200可见微量表达.HIBD组NF200阳性细胞6h出现表达,12h开始下降,24 h降到最低,此后逐渐升高.法舒地尔组各时间NF200的表达均高于HIBD组(P＜0.01),HIBD组和法舒地尔组NF200多于假手术组(P＜0.01).结论法舒地尔可能通过降低Nogo-A的表达和升高NF200的表达促进新生大鼠HIBD后神经细胞轴突的再生和修复.
목적 탐토법서지이대결양결혈성뇌손상(HIBD)대서Nogo수초상관단백(Nogo-A)급NF200표체적영향.방법 장120지신생7일령Wistar대서안수궤수자표법분가수술조(40지)、HIBD조(40지)화법서지이치료조(40지),가수술조지주경총동맥분리후불결찰,직접봉합절구불주결양처리,HIBD조조모후복강주사9 g/L염수,법서지이조복강주사법서지이(10 mg/kg).분별우술후6h、12 h、24 h、72 h화7d단경처사대서.HE염색경하관찰각조대서뇌조직병리변화.면역조직화학법검측뇌조직Nogo-A화NF200표체.결과 1.육안관찰:가수술조량측대뇌반구대칭;HIBD조수시간연장뇌조직수종가중,가견대뇌반구배사조;법서지이조뇌조직수종감경.2.HE염색:가수술조뇌조직결구형태정상;HIBD조가견세포종창,핵고축、렬해,염성세포증다;법서지이조신경세포수종급핵고축수량감소.3.면역조직화학:가수술조Nogo-A가견소량표체;HIBD조Nogo-A양성세포12h개시승고,24 h체봉치,수후표체축점강저;법서지이조제6h외,각시간점적표체균저우HIBD조(P ＜0.01);HIBD조화법서지이조양성세포수다우가수술조(P＜0.01).가수술조NF200가견미량표체.HIBD조NF200양성세포6h출현표체,12h개시하강,24 h강도최저,차후축점승고.법서지이조각시간NF200적표체균고우HIBD조(P＜0.01),HIBD조화법서지이조NF200다우가수술조(P＜0.01).결론 법서지이가능통과강저Nogo-A적표체화승고NF200적표체촉진신생대서HIBD후신경세포축돌적재생화수복.
Objective To study the effects of Fasudil on expression of Nogo-A and NF200 in neonatal hypoxic-ischemic brain damage(HIBD) rats.Methods One hundred and twenty 7-day-old Wistar rats were divided into 3 groups with random number table:Sham operation group (n =40),HIBD group (n =40) and Fasudil group (n =40).Sham group only separated from the common carotid artery,without ligation,direct suture the incision does not do hypoxia; HIBD group were injected with saline; Fasudil group was injected with fasudil(10 mg/kg).The rats were killed at 6 h,12 h,24 h,72 h,7 d,after administration.The pathological changes were observed by means of HE.The expression of Nogo-A and NF200 was studied with immunohistochemical staining.Results 1.Naked eye observation:Sham group bilateral symmetrical cerebral hemispheres; HIBD group of brain edema aggravated,the visible hemisphere focal necrosis; fasudil treatment group of edema than HIBD group ease.2.HE stain:the structure and shape of brain in Sham operation group were normal.In HIBD group,the cells became edema,karyopyknosis,lyse,and the inflammatory cells became more.The number of edema cells and karyopyknosis decreased in Fasudil group.3.Immunohistochemical stain:there were less expressions of Nogo-A in Sham operation group.It increased slightly after 12 h in HIBD group but decreased later.The expression of Nogo-A in Fasudil group was less than the other two groups at any time except 6 h (P ＜0.01).There was more expression in HIBD and Fasudil group compared with Sham operation group(P ＜0.01).NF200 was less expression in Sham operation group.NF200 appeared after 6 h and became less after 12 h.The expression of NF200 was at 24 h and later became more.The expression of NF200 in Fasudil group was more with HIBD group at each different time (P ＜ 0.01).The expressions of NF200 in Fasudil and HIBD group were more compared with Sham operation group.Conclusions Fasudil can rehabilitate the damaged axon and promote nerve regeneration through controlling the Rho/Rock and make the expression of NF200 increase.