CAJ | 학술논문

目的探讨大叶性肺炎患儿血清TNF-α、IL-6、IL-8、IL-10和高迁移率族蛋白B1(HMGB1)水平的变化及其相关性.方法采用双抗体夹心酶联免疫吸附法(ELISA)检测10例健康对照儿童、31例轻度大叶性肺炎患儿和14例重度大叶性肺炎患儿的血清TNF-α、IL-6、IL-8、IL-10和HMGB1水平,分析不同程度肺炎患儿血清HMGB1、TNF-α、IL-6、IL-8、IL-10水平的差异,及血清中TNF-α、IL-6、IL-8、IL-10与HMGB1水平的相关性.结果重度大叶性肺炎组患儿、轻度大叶性肺炎组患儿血清TNF-α、IL-6、IL-8、IL-10和HMGB1水平均显著高于健康对照组(P均＜0.05);重度大叶性肺炎组患儿和轻度大叶性肺炎患儿血清TNF-α、IL-6、IL-8、IL-10和HMGB1水平比较差异均有统计学意义(P均＜0.05).TNF-α、IL-6、IL-8与HMGB1呈中度正相关关系(r=0.444、0.365、0.466,P=0.007、0.029、0.004);IL-10与HMGB1呈中度负相关(r=-0.485,P=0.003).结论大叶性肺炎患儿血清TNF-α、IL-6、IL-8、IL-10、HMGB1与疾病严重程度相关,而TNF-α、IL-6、IL-8、IL-10、HMGB1水平测定可为小儿大叶性肺炎病情进展监测、严重程度及疗效判定等提供参考.
목적 탐토대협성폐염환인혈청TNF-α、IL-6、IL-8、IL-10화고천이솔족단백B1(HMGB1)수평적변화급기상관성.방법 채용쌍항체협심매련면역흡부법(ELISA)검측10례건강대조인동、31례경도대협성폐염환인화14례중도대협성폐염환인적혈청TNF-α、IL-6、IL-8、IL-10화HMGB1수평,분석불동정도폐염환인혈청HMGB1、TNF-α、IL-6、IL-8、IL-10수평적차이,급혈청중TNF-α、IL-6、IL-8、IL-10여HMGB1수평적상관성.결과 중도대협성폐염조환인、경도대협성폐염조환인혈청TNF-α、IL-6、IL-8、IL-10화HMGB1수평균현저고우건강대조조(P균＜0.05);중도대협성폐염조환인화경도대협성폐염환인혈청TNF-α、IL-6、IL-8、IL-10화HMGB1수평비교차이균유통계학의의(P균＜0.05).TNF-α、IL-6、IL-8여HMGB1정중도정상관관계(r=0.444、0.365、0.466,P=0.007、0.029、0.004);IL-10여HMGB1정중도부상관(r=-0.485,P=0.003).결론 대협성폐염환인혈청TNF-α、IL-6、IL-8、IL-10、HMGB1여질병엄중정도상관,이TNF-α、IL-6、IL-8、IL-10、HMGB1수평측정가위소인대협성폐염병정진전감측、엄중정도급료효판정등제공삼고.
Objective To investigate the concentration changes and the inter-relationship of tumor necrosis factor-α(TNF-α),interleukin-6 (IL-6),interleukin-8 (IL-8),interleukin-10 (IL-10) and high mobility group box-1 (HMGB1) in serum of children with lobar pneumonia.Methods By double-antibody sandwich enzyme linked immunosorbent assay,the serum TNF-α,IL-6,IL-8,IL-10,HMGB1 levels were determined in 10 healthy children,31 children with mild lobar pneumonia and 14 children with severe lobar pneumonia.The correlations between serum TNF-α,IL-6,IL-8,IL-10 with HMGB1 were analyzed.Results The levels of serum TNF-α,IL-6,IL-8,IL-10 and HMGB1 in children with severe lobar pneumonia and children with mild lobar pneumonia were significantly higher than those in healthy children (all P ＜ 0.05).There were significant differences in TNF-α,IL-6,IL-8,IL-10 and HMGB1 between children with mild lobar pneumonia and children with severe lobar pneumonia (all P ＜ 0.05).There was relationship between TNF-α,IL-6,IL-8 and HMGB1,showing moderate positive correlation,and the correlation coefficients were respectively 0.444,0.365 and 0.466,P =0.007,0.029,0.004;IL-10 and HMGB1 had moderate negative correlation,the correlation coefficient was 0.485,P =0.003.Conclusions For children with lobar pneumonia,serum concentration of TNF-α,IL-6,IL-8,IL-10 and HMGB1 were correlated with infectious status.To detect the serum level of TNF-α,IL-6,IL-8,IL-10 and HMGB1,can provide some reference for clinical practice.