中华实用儿科临床杂志
中華實用兒科臨床雜誌
중화실용인과림상잡지
Journal of Applied Clinical Pediatrics
2014年
16期
1246-1249
,共4页
刘晓鸣%岳璇%李瑞%陈盛植%陈娇
劉曉鳴%嶽璇%李瑞%陳盛植%陳嬌
류효명%악선%리서%진성식%진교
难治性癫(痫)%多药耐药相关蛋白%外周血单个核细胞%儿童
難治性癲(癇)%多藥耐藥相關蛋白%外週血單箇覈細胞%兒童
난치성전(간)%다약내약상관단백%외주혈단개핵세포%인동
Intractable epilepsy%Multidrug resistant associated protein%Peripheral blood mononuclear cell%Child
目的 研究多药耐药相关蛋白1、2(MRP1和MRP2)在难治性癫(痫)(IE)患儿外周血单个核细胞的表达.方法 收集2010年11月至2013年10月徐州市儿童医院门诊及住院的IE患儿50例,药物控制良好癫(痫)患儿50例,并以同期正常非癫(痫)儿童50例作为对照,应用反转录酶聚合酶链式反应(RT-PCR)和免疫蛋白印迹(Western blot)技术分析MRP1和MRP2基因及其蛋白在各组儿童外周血单个核细胞的表达.结果 1.1E患儿外周血单个核细胞MRP1和MRP2 mRNA相对表达量(0.795±0.042、0.804±0.023)明显高于正常非癫(痫)儿童(0.665±0.031、0.654±0.029)及药物控制良好癫(痫)患儿(0.682 ±0.030、0.675 ±0.021),差异均有统计学意义(P均＜0.001);2.IE患儿外周血单个核细胞MRP1和MRP2蛋白相对表达量(2.027±0.034、1.902 ±0.021)亦明显高于正常非癫(痫)儿童(1.093 ±0.023、1.045 ±0.018)及药物控制良好癫(痫)患儿(1.131±0.042、1.086±0.027),差异均有统计学意义(P均＜0.001);3.正常非癫(痫)儿童与药物控制良好癫(痫)患儿外周血单个核细胞MRP1和MRP2 mRNA及蛋白相对表达量比较差异均无统计学意义(P均＞0.05).结论 MRP1和MRP2在难治性癫(痫)患儿外周血单个核细胞中表达增强,可能与难治性癫(痫)耐药形成有关.
目的 研究多藥耐藥相關蛋白1、2(MRP1和MRP2)在難治性癲(癇)(IE)患兒外週血單箇覈細胞的錶達.方法 收集2010年11月至2013年10月徐州市兒童醫院門診及住院的IE患兒50例,藥物控製良好癲(癇)患兒50例,併以同期正常非癲(癇)兒童50例作為對照,應用反轉錄酶聚閤酶鏈式反應(RT-PCR)和免疫蛋白印跡(Western blot)技術分析MRP1和MRP2基因及其蛋白在各組兒童外週血單箇覈細胞的錶達.結果 1.1E患兒外週血單箇覈細胞MRP1和MRP2 mRNA相對錶達量(0.795±0.042、0.804±0.023)明顯高于正常非癲(癇)兒童(0.665±0.031、0.654±0.029)及藥物控製良好癲(癇)患兒(0.682 ±0.030、0.675 ±0.021),差異均有統計學意義(P均＜0.001);2.IE患兒外週血單箇覈細胞MRP1和MRP2蛋白相對錶達量(2.027±0.034、1.902 ±0.021)亦明顯高于正常非癲(癇)兒童(1.093 ±0.023、1.045 ±0.018)及藥物控製良好癲(癇)患兒(1.131±0.042、1.086±0.027),差異均有統計學意義(P均＜0.001);3.正常非癲(癇)兒童與藥物控製良好癲(癇)患兒外週血單箇覈細胞MRP1和MRP2 mRNA及蛋白相對錶達量比較差異均無統計學意義(P均＞0.05).結論 MRP1和MRP2在難治性癲(癇)患兒外週血單箇覈細胞中錶達增彊,可能與難治性癲(癇)耐藥形成有關.
목적 연구다약내약상관단백1、2(MRP1화MRP2)재난치성전(간)(IE)환인외주혈단개핵세포적표체.방법 수집2010년11월지2013년10월서주시인동의원문진급주원적IE환인50례,약물공제량호전(간)환인50례,병이동기정상비전(간)인동50례작위대조,응용반전록매취합매련식반응(RT-PCR)화면역단백인적(Western blot)기술분석MRP1화MRP2기인급기단백재각조인동외주혈단개핵세포적표체.결과 1.1E환인외주혈단개핵세포MRP1화MRP2 mRNA상대표체량(0.795±0.042、0.804±0.023)명현고우정상비전(간)인동(0.665±0.031、0.654±0.029)급약물공제량호전(간)환인(0.682 ±0.030、0.675 ±0.021),차이균유통계학의의(P균＜0.001);2.IE환인외주혈단개핵세포MRP1화MRP2단백상대표체량(2.027±0.034、1.902 ±0.021)역명현고우정상비전(간)인동(1.093 ±0.023、1.045 ±0.018)급약물공제량호전(간)환인(1.131±0.042、1.086±0.027),차이균유통계학의의(P균＜0.001);3.정상비전(간)인동여약물공제량호전(간)환인외주혈단개핵세포MRP1화MRP2 mRNA급단백상대표체량비교차이균무통계학의의(P균＞0.05).결론 MRP1화MRP2재난치성전(간)환인외주혈단개핵세포중표체증강,가능여난치성전(간)내약형성유관.
Objective To study the expression of multidrug resistance associated protein 1 (MRP1) and MRP2 in the peripheral blood mononuclear cells of children with intractable epilepsy (IE).Methods During Nov.2010 to Oct.2013,50 children with I E were collected as the experimental group,simultaneously 50 children with epilepsy controlled by the anti-epileptic drugs (AEDs) and 50 healthy children without epilepsy were collected as the control group from the outpatient or inpatient of Xuzhou Children's Hospital.The expressions of MRP1 and MRP2 in the peripheral blood mononuclear cells of children were detected by reverse transcriptase polymerase chain reaction and Western blot.Results 1.The mean relative expression of MRP1 and MRP2 mRNA in the peripheral blood mononuclear cells of children with IE (0.795 ± 0.042,0.804 ± 0.023) were higher than those in epilepsy controlled by AEDs (0.682 ± 0.030,0.675 ± 0.021) and healthy children without epilepsy (0.665 ± 0.031,0.654 ± 0.029) (all P ＜0.001).2.The mean relative expression of MRP1 and MRP2 protein in the peripheral blood mononuclear cells of children with IE (2.027 ±0.034,1.902 ±0.021) were higher than those in epilepsy controlled by AEDs(1.131 ±0.042,1.086 ± 0.027) and healthy children without epilepsy (1.093 ± 0.023,1.045 ± 0.018) (all P ＜ 0.001).3.There was no difference in the expression of MRP1,MRP2 mRNA and proteins between the children with epilepsy controlled by AEDs and healthy children without epilepsy (all P ＞ 0.05).Conclusions MRP1 and MRP2 over-expression in the peripheral blood mononuclear cells of children with IE may be associated with drug-resistance mechanism for medically intractable epilepsy.
