CAJ | 학술논문

目的研究早期运动干预对于高脂饮食诱导代谢性内毒素血症的影响,了解其对线粒体形态功能的改变.方法将100g左右的SPF级SD雄鼠随机分为A组(正常饮食组)、B组(高脂饮食组)及C组(高脂饮食联合运动组) . 于清洁级环境中饲养6周后处死,检测其体质量、血脂、血清内毒素水平.透射电镜了解其肝脏超微结构变化.差速离心法提取肝脏线粒体后,流式细胞仪检测其肝脏线粒体肿胀度及线粒体膜电位.结果饲养6周后,大鼠内毒素水平,B组相对于A组差异有统计学意义(2.916 ±0.761比5.454±1.254,t=-4.236,P＜0.05);透射电镜下观察B组细胞中的脂滴,较A、C组明显增多.但C组血清内毒素水平与B组比较,差异无统计学意义(4.972±1.757比5.454±1.254,t=-0.547,P ＞0.05).比较各组大鼠肝脏线粒体肿胀情况,B组相对于A、C组,差异均有统计学意义(4.330±0.501比3.507±0.532,t =2.759,P＜0.05;4.330±0.501比3.581±0.188,t=3.426,P＜0.05) . 各组大鼠肝脏线粒体膜电位情况,B、C组和A组比较,差异均有统计学意义(1.660±0.202比0.473±0.064,t=13.712,P ＜0.05;2.012±0.506比0.473±0.064,t=7.411,P ＜0.05).但各组透射电镜中线粒体形态并没有出现显并性差异.结论大鼠经高脂饮食喂养6周后可出现代谢性内毒素血症状态.代谢性内毒素对肝脏线粒体膜电位水平的影响,说明代谢性内毒素血症可能对未来疾病的发生发展产生影响.早期运动干预对循环中内毒素水平无明显影响,也从侧面说明高脂饮食所带来的危害并不能单纯通过运动来控制,合理膳食对于青少年键康尤其重要.
목적 연구조기운동간예대우고지음식유도대사성내독소혈증적영향,료해기대선립체형태공능적개변.방법 장100g좌우적SPF급SD웅서수궤분위A조(정상음식조)、B조(고지음식조)급C조(고지음식연합운동조) . 우청길급배경중사양6주후처사,검측기체질량、혈지、혈청내독소수평.투사전경료해기간장초미결구변화.차속리심법제취간장선립체후,류식세포의검측기간장선립체종창도급선립체막전위.결과 사양6주후,대서내독소수평,B조상대우A조차이유통계학의의(2.916 ±0.761비5.454±1.254,t=-4.236,P＜0.05);투사전경하관찰B조세포중적지적,교A、C조명현증다.단C조혈청내독소수평여B조비교,차이무통계학의의(4.972±1.757비5.454±1.254,t=-0.547,P ＞0.05).비교각조대서간장선립체종창정황,B조상대우A、C조,차이균유통계학의의(4.330±0.501비3.507±0.532,t =2.759,P＜0.05;4.330±0.501비3.581±0.188,t=3.426,P＜0.05) . 각조대서간장선립체막전위정황,B、C조화A조비교,차이균유통계학의의(1.660±0.202비0.473±0.064,t=13.712,P ＜0.05;2.012±0.506비0.473±0.064,t=7.411,P ＜0.05).단각조투사전경중선립체형태병몰유출현현병성차이.결론 대서경고지음식위양6주후가출현대사성내독소혈증상태.대사성내독소대간장선립체막전위수평적영향,설명대사성내독소혈증가능대미래질병적발생발전산생영향.조기운동간예대순배중내독소수평무명현영향,야종측면설명고지음식소대래적위해병불능단순통과운동래공제,합리선식대우청소년건강우기중요.
Objective To investigate the effect of concurrent exercise intervention in metabolic endotoxemia induced by high-fat diet in rats,and further understand the damage of liver mitochondrial ultramicrostructure.Methods Eighteen SD rats with the weight of 100g were randomly divided into 3 groups:group A (standard diet group),group B(high-fat diet group) and group C (treadmill-trained group with high-fat diet).Training (1 hour/d) initiated at the same time as the HF diet was fed.After being raised for 6 weeks,the rats was euthanized and weighed up.Blood samples were taken and the levels of serum lipid were detected.The levels of serum endotoxin were detected by enzyme-linked immunoadsorbent assay.The membrane potentials of isolated mitochondrion were detected by flow cytometry instrument and the morphologic changes in mitochondria in liver were observed by electronic microscopy.Results In group B,the levels of endotoxin increased significantly(2.916 ± 0.761 rs 5.454 ± 1.254,t =-4.236,P ＜ 0.05),and the liver mitochondrial density and membrane potential also increased significantly compared with group A after 6 weeks (4.330 ±0.501 vs 3.507 ±0.532,t =2.759,P ＜0.05;l.660 ±0.202 vs 0.473 ±0.064,t =13.712,P ＜0.05).But there was no markedly different in serum endotoxin between group B and group C (4.972 ± 1.757 vs 5.454 ± 1.254,t =-0.547,P ＞ 0.05).Compared with group B,the liver mitochondrial density of group C decreased significantly (4.330±0.501 vs 3.581 ±0.188,t =3.426,P ＜ 0.05).The mitochondrial ultrastructurctural changes in each group were not obvious.Conclusions The rats fed with high-fat diet for 6 weeks can reach the state of metabolic endotoxemia.The increasing levels of the liver mitochondrial membrane potential caused by metabolic endotoxin may affect the happening and development of other diseases in the future.Concurrent exercise can not decrease the level of endotoxin.It also shows that metabolic disease caused by high-fat diet should be prevented by moderation in eating and drinking.