CAJ | 학술논문

目的探讨髓样细胞触发受体1(TREM-1)在肺炎链球菌溶血素(PLY)致大鼠脑损伤中的动态变化及意义.方法将64只SD大鼠按简单随机法均等分为PLY组和对照组,分别经左侧颈内动脉注射0.1mL PLY和等体积灭菌9g/L盐水;观察注射后4h、6h、12 h和24 h不同时间点脑组织大体和组织学变化,并应用免疫组织化学法检测脑组织中神经细胞损伤标志物胶质纤维酸性蛋白(GFAP)和神经元特异性烯醇化酶(NSE)蛋白表达水平,同时应用酶联免疫吸附法检测TREM-1、肿瘤坏死因子-α(TNF-α)及白细胞介素6(IL-6)表达水平.结果 PLY组脑组织大体和组织学结果提示大鼠脑损伤存在,且脑组织中GFAP和NSE蛋白表达及TNF-α和IL-6表达在4h开始增加,随注射时间点推移,其表达水平动态增加,与相应时间点对照组相比,差异均有统计学意义(P均＜0.05).PLY组脑组织TREM-1蛋白表达在4h明显增加,在6h有所下降,但仍高于对照组,差异均有统计学意义(P均＜0.05),12 h与24 h TREM-1蛋白表达水平明显下降,与对照组相比差异无统计学意义(P均＞0.05).结论 TREM-1蛋白表达在PLY诱发的大鼠脑损伤早期明显上调,可能通过促进炎性因子TNF-α和IL-6的表达而参与脑损伤的病理发展过程.
목적 탐토수양세포촉발수체1(TREM-1)재폐염련구균용혈소(PLY)치대서뇌손상중적동태변화급의의.방법 장64지SD대서안간단수궤법균등분위PLY조화대조조,분별경좌측경내동맥주사0.1mL PLY화등체적멸균9g/L염수;관찰주사후4h、6h、12 h화24 h불동시간점뇌조직대체화조직학변화,병응용면역조직화학법검측뇌조직중신경세포손상표지물효질섬유산성단백(GFAP)화신경원특이성희순화매(NSE)단백표체수평,동시응용매련면역흡부법검측TREM-1、종류배사인자-α(TNF-α)급백세포개소6(IL-6)표체수평.결과 PLY조뇌조직대체화조직학결과제시대서뇌손상존재,차뇌조직중GFAP화NSE단백표체급TNF-α화IL-6표체재4h개시증가,수주사시간점추이,기표체수평동태증가,여상응시간점대조조상비,차이균유통계학의의(P균＜0.05).PLY조뇌조직TREM-1단백표체재4h명현증가,재6h유소하강,단잉고우대조조,차이균유통계학의의(P균＜0.05),12 h여24 h TREM-1단백표체수평명현하강,여대조조상비차이무통계학의의(P균＞0.05).결론 TREM-1단백표체재PLY유발적대서뇌손상조기명현상조,가능통과촉진염성인자TNF-α화IL-6적표체이삼여뇌손상적병리발전과정.
Objective To investigate the dynamic level changes and significance of triggering receptor expressed on myeloid cells-1 (TREM-1) in the injury brain tissues of rats caused by pneumolysin (PLY).Methods Sixty-four SD rats were randomly and equally divided into PLY group and control group,0.1 mL PLY and isopyknic normal saline was given through left internal carotid artery respectively.Brain tissue gross and histological changes were observed at different time(4 h,6 h,12 h,24 h),meanwhile the expression levels of neurocyte damage marker glial fibrillary acidic protein (GFAP) and neuron-specific enolase (NSE) protein were detected by immunohistochemistry;and the expression levels of TREM-1,tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were detected respectively by enzyme-linked immunosorbent assay.Results The observation of brain tissue gross and histological changes indicated the existence of brain injury,and the expression levels of GFAP,NSE,TNF-α and IL-6 protein increased from 4 h after PLY were injected and augmented dynamically as time went on,compared with the control group at corresponding time points,the differences were statistically significant (all P ＜ 0.05).The level of TREM-1 in the PLY group reached a peak at the 4 h time point,but decreased somewhat at the 6 h time point,the level of TREM-1 was still higher than that in control group,the differences were statistically significant(all P ＜ 0.05).However,the level of TREM-1 in the PLY group declined obviously at 12 h and 24 h time points,compared with that in control group,there were no significant differences (all P ＞ 0.05).Conclusions The expression levels of TREM-1 up-regulated obviously in the early stage of brain damage induced by PLY,which might be involved in the pathological process of brain damage by promoting the expression of TNF-α and IL-6.