CAJ | 학술논문

目的利用高脂饮食建立动物模型,观察孕前和孕期高脂饮食对新生仔鼠体格生长及胰岛素样生长因子1(IGF-1)的影响.方法 40只雌性SD大鼠采用简单随机抽样方法分为高脂组和对照组,分别喂养35％高脂饲料和普通饲料.喂养8周后,高脂组和对照组各取8只观察母鼠肝脏组织病理;其余与普通饲料喂养的雄性大鼠交配,孕期分别继续给予高脂饲料或普通饲料喂养.新生仔鼠出生24 h内检测各项指标:测量出生体质量和体长(鼻尾长度);酶联免疫吸附试验测定其血清IGF-1水平;生化分析仪检测血生化指标;光镜下观察肝脏组织病理;Western blot检测肝脏IGF-1蛋白的表达.结果 1.高脂组仔鼠的出生体质量和体长均较对照组显著降低(P均＜0.05).2.高脂组仔鼠血清IGF-1水平较对照组下降20.1％,但差异无统计学意义(P＞0.05).3.高脂组仔鼠的天冬氨酸转氨酶(AST)显著高于对照组(P＜0.05),其余各项血生化指标差异无统计学意义(P均＞0.05).4.高脂组母鼠及仔鼠肝组织可见脂肪变,而对照组肝脏病理形态正常.5.高脂组仔鼠肝脏IGF-1蛋白的表达高于对照组(P＜0.05).结论母亲孕前和孕期高脂饮食会影响胎鼠在宫内的体格生长,可能与IGF-1的下降有关,但导致血清IGF-1下降的病因以及宫内体格生长迟缓的确切病理机制尚有待进一步阐明.
목적 이용고지음식건립동물모형,관찰잉전화잉기고지음식대신생자서체격생장급이도소양생장인자1(IGF-1)적영향.방법 40지자성SD대서채용간단수궤추양방법분위고지조화대조조,분별위양35％고지사료화보통사료.위양8주후,고지조화대조조각취8지관찰모서간장조직병리;기여여보통사료위양적웅성대서교배,잉기분별계속급여고지사료혹보통사료위양.신생자서출생24 h내검측각항지표:측량출생체질량화체장(비미장도);매련면역흡부시험측정기혈청IGF-1수평;생화분석의검측혈생화지표;광경하관찰간장조직병리;Western blot검측간장IGF-1단백적표체.결과 1.고지조자서적출생체질량화체장균교대조조현저강저(P균＜0.05).2.고지조자서혈청IGF-1수평교대조조하강20.1％,단차이무통계학의의(P＞0.05).3.고지조자서적천동안산전안매(AST)현저고우대조조(P＜0.05),기여각항혈생화지표차이무통계학의의(P균＞0.05).4.고지조모서급자서간조직가견지방변,이대조조간장병리형태정상.5.고지조자서간장IGF-1단백적표체고우대조조(P＜0.05).결론 모친잉전화잉기고지음식회영향태서재궁내적체격생장,가능여IGF-1적하강유관,단도치혈청IGF-1하강적병인이급궁내체격생장지완적학절병리궤제상유대진일보천명.
Objective To establish neonate rate models induced by high-fat diet so as to explore the effect of high-fat diet before and during pregnancy on growth and serum insulin-like growth factor-1 (IGF-1) level in neonate offspring.Methods Forty female SD rats were divided into high-fat group and control group that were fed with 35％ high-fat diet and standard chow,respectively.After 8 weeks,8 rats from each group were euthanatized for liver pathology and the other female rats were mated with male rats and fed continuously with 35％ high-fat diet and standard chow throughout gestation,respectively.Offspring from both groups were studied within 24 hours after birth.Their birth weight and body length (from apex nasi to end of tail) were measured.Enzyme-linked immunosorbent assay was used to detect serum IGF-1 level.Serum biochemical indexes were tested by biochemical analyzer.Liver pathologic changes were observed under the light microscope.The expression of IGF-1 in liver samples was detected by Western blot.Results 1.Rats in the high-fat group showed a significant less birth weight and shorter body length compared with those in the control group(all P ＜ 0.05).2.The level of serum IGF-1 of offspring in the high-fat group decreased by 20.1％ in comparison to offspring in the control group,but there was no significant difference between the 2 groups (P ＞ 0.05) because of the limited sample sizes.3.The aspartate transaminase(AST) of offspring in the high-fat group increased compared with those from control group(P ＜ 0.05),while other serum biochemical indexes between the 2 groups showed no significant differences(all P ＞0.05).4.Fatty degeneration could be found in livers of both dams and offspring in the high-fat group under light microscope,while the samples of both dams and offspring from the control group all showed normal.5.The IGF-1 expression in livers of offspring from high-fat group increased in comparison to that from control group(P ＜ 0.05).Conclusions A maternal high-fat diet before and during pregnancy decreases the birth weight and body length of offspring in utero,which may be associated with the decreased IGF-1.However,the pathogenesis of decreased IGF-1 in this study can not been found.Further study is needed to clarify the mechanism of intrauterine growth retardation induced by high-fat diet.