CAJ | 학술논문

目的比较采取腹腔注射、饮水方式与灌胃方式补充叶酸对细菌脂多糖(LPS)诱发胎鼠畸形的预防效应.方法将孕鼠89只随机分为6组,叶酸对照组16只、生理盐水对照组16只、LPS组16只、LPS+叶酸灌胃组16只、LPS+叶酸腹腔注射组13只、LPS+叶酸饮水组12只,于孕第8～12天给予相应的药物处理.于孕第18天剖杀,记录活胎鼠数、死胎鼠数、吸收胎数,判断活胎鼠外观畸形情况,并对胎鼠骨骼畸形进行评价.结果孕中期小鼠暴露于低剂量LPS可引起胎鼠外观和骨骼畸形,外观畸形以露脑和脑膜膨出最常见,骨骼畸形主要表现为枕骨骨化不全、胸骨畸形和肋骨畸形,胎鼠外观畸形发生率、骨骼畸形发生率在补充叶酸后均有明显降低,灌胃方式补充叶酸效果最明显,每窝外观畸形率、胸骨异常畸胎率、肋骨异常畸胎率均显著降低.结论 3种给药方式对于孕中期LPS暴露所诱发的胎鼠外观畸形和骨骼畸形均有预防作用,其中以灌胃方式效果最佳.
목적 비교채취복강주사、음수방식여관위방식보충협산대세균지다당(LPS)유발태서기형적예방효응.방법 장잉서89지수궤분위6조,협산대조조16지、생리염수대조조16지、LPS조16지、LPS+협산관위조16지、LPS+협산복강주사조13지、LPS+협산음수조12지,우잉제8～12천급여상응적약물처리.우잉제18천부살,기록활태서수、사태서수、흡수태수,판단활태서외관기형정황,병대태서골격기형진행평개.결과 잉중기소서폭로우저제량LPS가인기태서외관화골격기형,외관기형이로뇌화뇌막팽출최상견,골격기형주요표현위침골골화불전、흉골기형화륵골기형,태서외관기형발생솔、골격기형발생솔재보충협산후균유명현강저,관위방식보충협산효과최명현,매와외관기형솔、흉골이상기태솔、륵골이상기태솔균현저강저.결론 3충급약방식대우잉중기LPS폭로소유발적태서외관기형화골격기형균유예방작용,기중이관위방식효과최가.
Objective To explore the protective effect of folic acid supplementation in different ways on lipopolysaccharide-induced external malformations and skeletal malformations in mice.Methods The pregnant mice were divided into six groups randomly,including saline control group,FA control group,LPS group,LPS+FAig group,LPS+FAip group,and LPS+FAdw group.All dams were medicated on gestational day 8～12,and then sacrificed on gestational day 18,for each litter,the number of live fetuses,dead fetuses,resorption fetuses and external malformations of live fetuses were all counted.All fetuses were subsequently evaluated the skeletal malformations.Results A low dose of LPS injection during the second trimester resulted in external and skeletal malformations.Exencephaly and encephalomeningocele were two of the most common external malformations,skeletal malformations consisted mainly of the incompletion of supraoccipital ossification,sternal malformation and rib malformation.FA supplementation by three ways all attenuated the external and skeletal malformations.And the best protective effect was by oral administration.The incidence of external,sternal and rib malformation was all decreased.Conclusions Three ways can prevent the mice during the second trimester from the external and skeletal malformations caused by LPS injection,and the best protective effect was by intragastric administration.