CAJ | 학술논문

目的检测乳腺癌患者外周血中细胞角蛋白19 (cytokeratin 19,CK19) mRNA的表达,探讨其表达是否可作为判断乳腺癌外周血微转移及预后的分子生物学指标.方法采用逆转录-多聚酶链反应(reverse transcription polymerase chain reaction,RT-PCR)技术检测75例乳腺癌患者、21例乳腺良性病变患者和20例健康者外周血中CK19 mRNA的表达情况.结果 CK19 mRNA在乳腺癌患者、乳腺良性病变患者和健康者血清中阳性率分别为36％、0％和0％.乳腺癌患者和乳腺良性肿瘤患者及健康者血清中CK19 mRNA表达阳性率差异有统计学上的意义(P＜0.05).CK19mRNA表达阳性率和临床分期呈正相关(P＜0.05).结论乳腺癌患者外周血中CK19 mRNA表达与临床分期密切相关,提示该项指标有望预测乳腺癌外周血微转移和判断预后.
목적 검측유선암환자외주혈중세포각단백19 (cytokeratin 19,CK19) mRNA적표체,탐토기표체시부가작위판단유선암외주혈미전이급예후적분자생물학지표.방법 채용역전록-다취매련반응(reverse transcription polymerase chain reaction,RT-PCR)기술검측75례유선암환자、21례유선량성병변환자화20례건강자외주혈중CK19 mRNA적표체정황.결과 CK19 mRNA재유선암환자、유선량성병변환자화건강자혈청중양성솔분별위36％、0％화0％.유선암환자화유선량성종류환자급건강자혈청중CK19 mRNA표체양성솔차이유통계학상적의의(P＜0.05).CK19mRNA표체양성솔화림상분기정정상관(P＜0.05).결론 유선암환자외주혈중CK19 mRNA표체여림상분기밀절상관,제시해항지표유망예측유선암외주혈미전이화판단예후.
Objective To detect the expression of CK19 mRNA in peripheral blood in patients with breast carcinoma and to explore whether this expression can be used as a molecular biological marker for predicting micrometastasis and prognosis.Methods Peripheral serum samples were collected from 75 patients with breast carcinoma,21 patients with benign breast lesions,and 20 healthy subjects.Expression of CK19 mRNA was detected by RT-PCR.Results The positive rate of CK 19 mRNA was 36.0％ in patients with breast carcinoma and 0％ in those with benign breast lesions and in healthy subjects; and there were significant differences in the expression of CK19 mRNA among patients with breast carcinoma,those with benign breast lesions,and healthy subjects (P＜0.001).The positive rate of CK19 mRNA positively correlated with the clinical stages (P＜0.05).Conclusions CK19 mRNA can be detected in peripheral serum of breast carcinoma patients.CK19 mRNA expression closely correlates with the clinical stages in patients with breast carcinoma,suggesting this index can be used to predict micrometastasis and prognosis.