中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2012年
4期
269-272
,共4页
李玲%刘风华%石宇%吴菁%刘怡潼%农璎琦%易艳红%董梅
李玲%劉風華%石宇%吳菁%劉怡潼%農瓔琦%易豔紅%董梅
리령%류풍화%석우%오정%류이동%농영기%역염홍%동매
流产,习惯性%黄体期%子宫内膜%血管内皮生长因子%孕激素受体
流產,習慣性%黃體期%子宮內膜%血管內皮生長因子%孕激素受體
유산,습관성%황체기%자궁내막%혈관내피생장인자%잉격소수체
Abortion,habitual%Luteal phase%Endometrium%Vascular endothelial growth factor%Progesterone receptor
目的 检测不明原因复发性流产(URSA)患者分泌中期子宫内膜的血管内皮生长因子(VEGF)和孕激素受体(PR) mRNA及蛋白的表达及探讨其意义.方法 选取2010年6月至2011年10月在广州医学院第三附属医院生殖医学中心和广东省妇幼保健院生殖医学中心及产前诊断科的22例URSA患者为研究组;20例正常妇女为对照组.采用实时荧光定量PCR和免疫组化法检测2组人群分泌中期子宫内膜VEGF和PR的mRNA及蛋白的相对表达水平.结果 VEGF和PR的mRNA和蛋白在两组表达率均为100％;VEGF蛋白表达于腔上皮和间质;PR蛋白表达于腺上皮和间质.研究组VEGF的mRNA和蛋白表达水平均低于对照组(0.16±0.10比0.34±0.22,0.014±0.004比0.018±0.005,均P＜0.05);研究组PR的mRNA和蛋白表达水平均低于对照组(2.52±0.99比4.38± 1.44,0.25±0.02比0.32±0.02,均P＜0.05).结论 URSA患者分泌中期VEGF、PR的相对低表达可能是导致URSA的分子机制之一.
目的 檢測不明原因複髮性流產(URSA)患者分泌中期子宮內膜的血管內皮生長因子(VEGF)和孕激素受體(PR) mRNA及蛋白的錶達及探討其意義.方法 選取2010年6月至2011年10月在廣州醫學院第三附屬醫院生殖醫學中心和廣東省婦幼保健院生殖醫學中心及產前診斷科的22例URSA患者為研究組;20例正常婦女為對照組.採用實時熒光定量PCR和免疫組化法檢測2組人群分泌中期子宮內膜VEGF和PR的mRNA及蛋白的相對錶達水平.結果 VEGF和PR的mRNA和蛋白在兩組錶達率均為100％;VEGF蛋白錶達于腔上皮和間質;PR蛋白錶達于腺上皮和間質.研究組VEGF的mRNA和蛋白錶達水平均低于對照組(0.16±0.10比0.34±0.22,0.014±0.004比0.018±0.005,均P＜0.05);研究組PR的mRNA和蛋白錶達水平均低于對照組(2.52±0.99比4.38± 1.44,0.25±0.02比0.32±0.02,均P＜0.05).結論 URSA患者分泌中期VEGF、PR的相對低錶達可能是導緻URSA的分子機製之一.
목적 검측불명원인복발성유산(URSA)환자분비중기자궁내막적혈관내피생장인자(VEGF)화잉격소수체(PR) mRNA급단백적표체급탐토기의의.방법 선취2010년6월지2011년10월재엄주의학원제삼부속의원생식의학중심화광동성부유보건원생식의학중심급산전진단과적22례URSA환자위연구조;20례정상부녀위대조조.채용실시형광정량PCR화면역조화법검측2조인군분비중기자궁내막VEGF화PR적mRNA급단백적상대표체수평.결과 VEGF화PR적mRNA화단백재량조표체솔균위100％;VEGF단백표체우강상피화간질;PR단백표체우선상피화간질.연구조VEGF적mRNA화단백표체수평균저우대조조(0.16±0.10비0.34±0.22,0.014±0.004비0.018±0.005,균P＜0.05);연구조PR적mRNA화단백표체수평균저우대조조(2.52±0.99비4.38± 1.44,0.25±0.02비0.32±0.02,균P＜0.05).결론 URSA환자분비중기VEGF、PR적상대저표체가능시도치URSA적분자궤제지일.
Objective To examine the expressions of vascular endothelial growth factor (VEGF) and progesterone receptor (PR) in ednometrium of mid- secretory phase in patients with unexplained recurrent spontaneous abortion (URSA),and to explore their significance.Methods Between June 2010 and October 2011,22 URSA cases were selected and recruited into research group,and 20 normal women were assigned into control group in Department of Reproductive Medical Center,Guangdong Women and Children Hospital and the Third Hospital of Guangzhou Medical College.Real time quantitative fluorescence PCR (RTQ-PCR) and immunohistochemistry were used to detect the relative expressions of VEGF and PR in endometrium of mid-secretory phase in two groups.Results The expression rates of mRNA and protein of VEGF and PR were 100％ in both two groups.The protein expression of VEGF was in luminal epithelium and mesenchyma and that of PR in glandular epithelium and mesenchyma.The expression levels of mRNA and protein of VEGF and PR in research group were significantly lower than those in control group (0.16±0.10 vs 0.34±0.22; 0.014±0.004 vs 0.018±0.005;2.52±0.99 vs 4.38± 1.44; 0.25±0.02 vs 0.32±0.02,all P＜0.05).Conclusion The relatively lower expression levels of VEGF and PR in endometrium of mid-secretory phase may be one of the mechanisms to cause URSA.
