中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2012年
5期
388-390
,共3页
细胞培养技术%杀伤细胞%水浴
細胞培養技術%殺傷細胞%水浴
세포배양기술%살상세포%수욕
Cell culture techniques%Killer cells%Water-bath
目的 对比干式融化箱与水浴锅复苏冻存细胞因子诱导的杀伤细胞(CIK细胞)的效果.方法 提取10份健康志愿者外周血单个核细胞分别诱导培养CIK细胞并冻存于-80℃冰箱.1个月后从每份CIK细胞中取出相同的两个冻存管,分别采用干式融化箱和水浴锅进行复苏.记录复苏时间,检测复苏后细胞存活率及对K562细胞的杀伤作用.结果 干式融化箱较水浴锅复苏时间较长[(4.33±0.19)min、(2.47±0.30) min],差异有统计学意义(P＜0.05).但两种方法复苏的CIK细胞存活率[(89.55±5.36)％、(89.86±4.63)％]和对K562细胞的杀伤活性[(44.76±9.53)％、(46.97±10.17)％]差异无统计学意义(P＞0.05).结论 干式融化箱可以代替水浴锅进行冻存CIK细胞复苏.
目的 對比榦式融化箱與水浴鍋複囌凍存細胞因子誘導的殺傷細胞(CIK細胞)的效果.方法 提取10份健康誌願者外週血單箇覈細胞分彆誘導培養CIK細胞併凍存于-80℃冰箱.1箇月後從每份CIK細胞中取齣相同的兩箇凍存管,分彆採用榦式融化箱和水浴鍋進行複囌.記錄複囌時間,檢測複囌後細胞存活率及對K562細胞的殺傷作用.結果 榦式融化箱較水浴鍋複囌時間較長[(4.33±0.19)min、(2.47±0.30) min],差異有統計學意義(P＜0.05).但兩種方法複囌的CIK細胞存活率[(89.55±5.36)％、(89.86±4.63)％]和對K562細胞的殺傷活性[(44.76±9.53)％、(46.97±10.17)％]差異無統計學意義(P＞0.05).結論 榦式融化箱可以代替水浴鍋進行凍存CIK細胞複囌.
목적 대비간식융화상여수욕과복소동존세포인자유도적살상세포(CIK세포)적효과.방법 제취10빈건강지원자외주혈단개핵세포분별유도배양CIK세포병동존우-80℃빙상.1개월후종매빈CIK세포중취출상동적량개동존관,분별채용간식융화상화수욕과진행복소.기록복소시간,검측복소후세포존활솔급대K562세포적살상작용.결과 간식융화상교수욕과복소시간교장[(4.33±0.19)min、(2.47±0.30) min],차이유통계학의의(P＜0.05).단량충방법복소적CIK세포존활솔[(89.55±5.36)％、(89.86±4.63)％]화대K562세포적살상활성[(44.76±9.53)％、(46.97±10.17)％]차이무통계학의의(P＞0.05).결론 간식융화상가이대체수욕과진행동존CIK세포복소.
Objective To compare the effects of dry and conventional water-bath approaches on thawing cryopreserved cytokine-induced killer (CIK) cells.Methods Peripheral blood monocytes (PBMCs) isolated from 10 healthy donors were induced to obtain CIK cells and cryopreserved in a-80 ℃ freezer.An aliquot of CIK cells was harvested from two cryopreserved tubes and subjected to dry and conventional water-bath thawing at month 1 respectively.The time of thawing was recorded and the survival rate and cytotoxity agaist K562 cells were measured.Results Dry thawing yielded a prolonged duration as compared with water-bath [(4.33 ±0.19)min vs (2.47 ±0.30)min,P＜0.05].However,the difference in survival rate [(89.55±5.36％) vs (89.86±4.63％)] and cytotoxity of CIK cells [(44.76±9.53％) vs (46.97±10.17％)] agaist K562 cells did not reach statistical significance (bothP＞0.05).Conclusion Dry thawing may be deemed as a surrogate for conventional water-bath thawing of cryopreserved CIK cells.
