中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2012年
5期
362-365
,共4页
李洪涛%张天托%黄静%刘慧%谢和平%潘明安
李洪濤%張天託%黃靜%劉慧%謝和平%潘明安
리홍도%장천탁%황정%류혜%사화평%반명안
肺疾病,慢性阻塞性%冬虫夏草%N-乙酰半胱氨酸%细胞因子%谷胱甘肽
肺疾病,慢性阻塞性%鼕蟲夏草%N-乙酰半胱氨痠%細胞因子%穀胱甘肽
폐질병,만성조새성%동충하초%N-을선반광안산%세포인자%곡광감태
Pulmonary disease,chronic obstructive%Cordyceps sinensis%N-Acetylcysteine%Cytokines%Glutathione
目的 探讨冬虫夏草对大鼠慢性阻塞性肺疾病(COPD)模型还原型谷胱甘肽(GSH)-氧化型谷胱甘肽(GSSG)失衡的干预作用及对Th1-Th2型细胞因子的影响.方法 18只SD大鼠完全随机分为COPD对照组、冬虫夏草干预组和N-乙酰半胱氨酸(NAC)干预组(每组各6只),分别在COPD模型制作中胃饲生理盐水、冬虫夏草和NAC,观察大鼠肺组织病理改变.检测冬虫夏草和NAC干预后支气管肺泡灌洗液(BALF)中Th1-Th2型细胞因子和巨噬细胞中GSH和GSH/GSSG变化.结果 与COPD对照组(32±13)相比,冬虫夏草干预组和NAC干预组的平均肺泡计数增加(49±10,52±14,P＜0.05).冬虫夏草干预组和NAC干预组BALF中巨噬细胞GSH水平和GSH-GSSG较COPD对照组升高(t=3.06,t=3.24; t=2.36,t=2.82;均P＜0.05).冬虫夏草干预组和NAC干预组BALF上清液中Th1型细胞因子IFN-γ水平较COPD对照组升高(t=2.34,t=2.32,P＜0.05).结论 冬虫夏草可能通过提高COPD模型巨噬细胞内GSH水平,补充抗氧化水平而达到纠正Th1-Th2失衡的免疫调节.
目的 探討鼕蟲夏草對大鼠慢性阻塞性肺疾病(COPD)模型還原型穀胱甘肽(GSH)-氧化型穀胱甘肽(GSSG)失衡的榦預作用及對Th1-Th2型細胞因子的影響.方法 18隻SD大鼠完全隨機分為COPD對照組、鼕蟲夏草榦預組和N-乙酰半胱氨痠(NAC)榦預組(每組各6隻),分彆在COPD模型製作中胃飼生理鹽水、鼕蟲夏草和NAC,觀察大鼠肺組織病理改變.檢測鼕蟲夏草和NAC榦預後支氣管肺泡灌洗液(BALF)中Th1-Th2型細胞因子和巨噬細胞中GSH和GSH/GSSG變化.結果 與COPD對照組(32±13)相比,鼕蟲夏草榦預組和NAC榦預組的平均肺泡計數增加(49±10,52±14,P＜0.05).鼕蟲夏草榦預組和NAC榦預組BALF中巨噬細胞GSH水平和GSH-GSSG較COPD對照組升高(t=3.06,t=3.24; t=2.36,t=2.82;均P＜0.05).鼕蟲夏草榦預組和NAC榦預組BALF上清液中Th1型細胞因子IFN-γ水平較COPD對照組升高(t=2.34,t=2.32,P＜0.05).結論 鼕蟲夏草可能通過提高COPD模型巨噬細胞內GSH水平,補充抗氧化水平而達到糾正Th1-Th2失衡的免疫調節.
목적 탐토동충하초대대서만성조새성폐질병(COPD)모형환원형곡광감태(GSH)-양화형곡광감태(GSSG)실형적간예작용급대Th1-Th2형세포인자적영향.방법 18지SD대서완전수궤분위COPD대조조、동충하초간예조화N-을선반광안산(NAC)간예조(매조각6지),분별재COPD모형제작중위사생리염수、동충하초화NAC,관찰대서폐조직병리개변.검측동충하초화NAC간예후지기관폐포관세액(BALF)중Th1-Th2형세포인자화거서세포중GSH화GSH/GSSG변화.결과 여COPD대조조(32±13)상비,동충하초간예조화NAC간예조적평균폐포계수증가(49±10,52±14,P＜0.05).동충하초간예조화NAC간예조BALF중거서세포GSH수평화GSH-GSSG교COPD대조조승고(t=3.06,t=3.24; t=2.36,t=2.82;균P＜0.05).동충하초간예조화NAC간예조BALF상청액중Th1형세포인자IFN-γ수평교COPD대조조승고(t=2.34,t=2.32,P＜0.05).결론 동충하초가능통과제고COPD모형거서세포내GSH수평,보충항양화수평이체도규정Th1-Th2실형적면역조절.
Objective To investigate the effects of Cordyceps sinensis (C.sinensis) on reduced/oxidized glutathione imbalance and Th 1/Th2 cytokines in rat models with chronic obstructive pulmonary disease (COPD).Methods A total of 18 Sprague-Dawley male rats was randomly assigned to receive normal saline(control group,n=6),C.sinensis(C.sinensis intervention group,n=6) or N-acetylcysteine(NAC intervention group,n=6) feeding for determination of pathological changes in lung tissues.Bronchoalveolar lavage fluid(BALF) was collected to determine the changes in Th1/Th2 cytokines and variation in reduced/oxidized glutathione within macrophages after interventions of C.sinensis and NAC.Results Both C.sinensis and NAC resulted in increased alveolar cell count and the ratio of reduced to oxidized glutathione within macrophages obtained from BALF as compared with the counterpart controls(49±10 and 52±14 vs 32±13 ; t=3.06,t=3.24; t=2.36,t=2.82,all P＜0.05].Higher levels of IFN-γand Th1 cytokine were found in BALF supernatant of C.sinensis and NAC intervention groups (t=2.34,t=2.32,both P＜0.05).Conclusion C.sinensis may present immunomodulatory effects on Th1/Th2 imbalance via up-regulation of glutathione within macrophages in COPD models and supplementation of anti-oxidization capacity.
