中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2012年
5期
384-387
,共4页
张伟雯%黄月玲%方敏华%孙卫文%李敏雄%戴丽军%陈盛强
張偉雯%黃月玲%方敏華%孫衛文%李敏雄%戴麗軍%陳盛彊
장위문%황월령%방민화%손위문%리민웅%대려군%진성강
脆性X综合征%小鼠,基因敲除%避暗实验%跳台实验
脆性X綜閤徵%小鼠,基因敲除%避暗實驗%跳檯實驗
취성X종합정%소서,기인고제%피암실험%도태실험
Fragile X syndrome%Mice,knockout%Step-through test%Step-down test
目的 观察30日龄的脆性X基因(Fmr1)敲除小鼠(KO鼠)的被动回避行为.方法 选取Fmr1基因敲除型(KO)纯合子(-/-)及其野生型(WT)纯合子(+/+)FVB近交系小鼠30日龄各15只,采用PCR鉴定实验动物KO鼠和WT鼠的基因型,进行2d的被动回避行为的避暗和跳台实验,比较2组小鼠电击次数和潜伏期时间.结果 避暗实验中,第1天KO鼠的电击次数为(8.28±0.50)次,与WT鼠(7.10±0.46)次相比显著增多(P＜0.05)；潜伏期时间虽增加但差异无统计学意义.第2天KO鼠的电击次数及潜伏期时间与WT鼠相比,差异均无统计学意义.KO和WT鼠第1天与第2天的潜伏期和电击次数相比,差异均有统计学意义(均P＜0.05).跳台实验中,第1天KO鼠的潜伏期为(94.96±10.56)s,比WT鼠(135.73±12.17)s明显缩短(P＜0.05)；而电击次数为(1.81±0.31)次,比WT鼠(0.67±0.13)次明显增多(P＜0.05).第2天KO鼠的电击次数及潜伏期时间与WT鼠相比,差异也无统计学意义.第1天WT鼠的电击次数与第2天相比,差异无统计学意义,潜伏期则低于第2天(P＜0.05)；而第1天KO鼠的潜伏期和电击次数与第2天相比,差异有统计学意义(均P＜0.05).结论 30日龄Fmr1基因敲除小鼠存在认知功能障碍.
目的 觀察30日齡的脆性X基因(Fmr1)敲除小鼠(KO鼠)的被動迴避行為.方法 選取Fmr1基因敲除型(KO)純閤子(-/-)及其野生型(WT)純閤子(+/+)FVB近交繫小鼠30日齡各15隻,採用PCR鑒定實驗動物KO鼠和WT鼠的基因型,進行2d的被動迴避行為的避暗和跳檯實驗,比較2組小鼠電擊次數和潛伏期時間.結果 避暗實驗中,第1天KO鼠的電擊次數為(8.28±0.50)次,與WT鼠(7.10±0.46)次相比顯著增多(P＜0.05)；潛伏期時間雖增加但差異無統計學意義.第2天KO鼠的電擊次數及潛伏期時間與WT鼠相比,差異均無統計學意義.KO和WT鼠第1天與第2天的潛伏期和電擊次數相比,差異均有統計學意義(均P＜0.05).跳檯實驗中,第1天KO鼠的潛伏期為(94.96±10.56)s,比WT鼠(135.73±12.17)s明顯縮短(P＜0.05)；而電擊次數為(1.81±0.31)次,比WT鼠(0.67±0.13)次明顯增多(P＜0.05).第2天KO鼠的電擊次數及潛伏期時間與WT鼠相比,差異也無統計學意義.第1天WT鼠的電擊次數與第2天相比,差異無統計學意義,潛伏期則低于第2天(P＜0.05)；而第1天KO鼠的潛伏期和電擊次數與第2天相比,差異有統計學意義(均P＜0.05).結論 30日齡Fmr1基因敲除小鼠存在認知功能障礙.
목적 관찰30일령적취성X기인(Fmr1)고제소서(KO서)적피동회피행위.방법 선취Fmr1기인고제형(KO)순합자(-/-)급기야생형(WT)순합자(+/+)FVB근교계소서30일령각15지,채용PCR감정실험동물KO서화WT서적기인형,진행2d적피동회피행위적피암화도태실험,비교2조소서전격차수화잠복기시간.결과 피암실험중,제1천KO서적전격차수위(8.28±0.50)차,여WT서(7.10±0.46)차상비현저증다(P＜0.05)；잠복기시간수증가단차이무통계학의의.제2천KO서적전격차수급잠복기시간여WT서상비,차이균무통계학의의.KO화WT서제1천여제2천적잠복기화전격차수상비,차이균유통계학의의(균P＜0.05).도태실험중,제1천KO서적잠복기위(94.96±10.56)s,비WT서(135.73±12.17)s명현축단(P＜0.05)；이전격차수위(1.81±0.31)차,비WT서(0.67±0.13)차명현증다(P＜0.05).제2천KO서적전격차수급잠복기시간여WT서상비,차이야무통계학의의.제1천WT서적전격차수여제2천상비,차이무통계학의의,잠복기칙저우제2천(P＜0.05)；이제1천KO서적잠복기화전격차수여제2천상비,차이유통계학의의(균P＜0.05).결론 30일령Fmr1기인고제소서존재인지공능장애.
Objective To investigate the passive avoidance behavior in Fmr1-knockout (KO) mice at 30 days of age.Methods Fmr1-KO mice(-/-,n=15) and wild-type FVB inbreeded (WT) mice (+/+,n=15) at 30 days of age were allocated and subjected to genotyping by using polymerase chain reaction.Stepthrough and step-down tests were conducted for measurement of latency period and the number of electric shocks.Results During the step-through test,KO mice had higher incidence of electric shocks (8.28±0.50vs 7.10±0.46,P＜0.05) than WT mice at day 1.There was an increased trend of latency period,but the between-group difference did not reach statistical significance.At day 2,there was no marked between-group difference in the latency period and number of electric shock.Additionally,the latency period and number of electric shock did differ considerably between day 1 and day 2(all P＜0.05).During the step-down test,KO mice yielded impressively shorter latency period [(94.96±10.56)s vs (135.73±12.17)s,P＜0.05] yet higher incidence of electric shock (1.81 ± 0.31 vs 0.67 ± 0.13,P＜0.05) compared with WT mice.Between-group difference in the latency period and the number of electric shock did not reach statistical significance at day 2.There was no significant difference in the number of electric shock of WT mice between days 1 and 2.The latency period of WT mice was shorter than that at day 2(P＜0.05).However,the latency period and number of electric shocks of KO mice differed significantly between days 1 and 2(all P＜0.05).Conclusion Fmr1-knockout mice at 30 days of age may have developed cognitive impairment.
