中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2012年
6期
441-444
,共4页
胡燕荣%高莉%杨海%康永安%周玲%李南方
鬍燕榮%高莉%楊海%康永安%週玲%李南方
호연영%고리%양해%강영안%주령%리남방
疾病模型,动物%G蛋白耦联的内向整流型K离子通道蛋白4%肥胖症
疾病模型,動物%G蛋白耦聯的內嚮整流型K離子通道蛋白4%肥胖癥
질병모형,동물%G단백우련적내향정류형K리자통도단백4%비반증
Disease models,animal%C-coupled-inwardly-rectifying K current,CIRK%Obesity
目的 研究饮食诱导的肥胖大鼠模型下丘脑、肝、胰等组织器官中G蛋白耦联的内向整流型K离子通道蛋白4(GIRK4)的表达.方法 30只SD大鼠按随机数字表分两组,肥胖大鼠组(20只,高脂高糖饮食构建肥胖大鼠模型)和正常对照组(10只,正常饮食).建模成功处死两组大鼠后分离下丘脑、肝、胰等组织器官,提取蛋白,Western免疫印迹检测GIRK4表达.对肥胖大鼠组与正常对照组大鼠体质量、体长、脂肪系数等指标进行分析比较.结果 肥胖大鼠组大鼠体长、体质量、脂肪系数显著高于正常对照组[雌:(22.00±0.71)比(20.80±0.45)cm,(289.10±29.06)比(239.60±10.08)g,(3.47±1.54)比(1.14±0.56)；雄:(26.32±0.87)比(24.80±0.84)em,(432.00±28.14)比(352.00±37.37)g,(3.20±0.56)比(1.11±0.27)；均P＜0.05],但下丘脑、肝、胰脏的GIRK4表达却显著低于正常对照组(P＜0.05).结论 大鼠下丘脑、肝脏、胰脏较高表达的GIRK4可能与脂肪代谢、饮食诱导型肥胖的发生有关.
目的 研究飲食誘導的肥胖大鼠模型下丘腦、肝、胰等組織器官中G蛋白耦聯的內嚮整流型K離子通道蛋白4(GIRK4)的錶達.方法 30隻SD大鼠按隨機數字錶分兩組,肥胖大鼠組(20隻,高脂高糖飲食構建肥胖大鼠模型)和正常對照組(10隻,正常飲食).建模成功處死兩組大鼠後分離下丘腦、肝、胰等組織器官,提取蛋白,Western免疫印跡檢測GIRK4錶達.對肥胖大鼠組與正常對照組大鼠體質量、體長、脂肪繫數等指標進行分析比較.結果 肥胖大鼠組大鼠體長、體質量、脂肪繫數顯著高于正常對照組[雌:(22.00±0.71)比(20.80±0.45)cm,(289.10±29.06)比(239.60±10.08)g,(3.47±1.54)比(1.14±0.56)；雄:(26.32±0.87)比(24.80±0.84)em,(432.00±28.14)比(352.00±37.37)g,(3.20±0.56)比(1.11±0.27)；均P＜0.05],但下丘腦、肝、胰髒的GIRK4錶達卻顯著低于正常對照組(P＜0.05).結論 大鼠下丘腦、肝髒、胰髒較高錶達的GIRK4可能與脂肪代謝、飲食誘導型肥胖的髮生有關.
목적 연구음식유도적비반대서모형하구뇌、간、이등조직기관중G단백우련적내향정류형K리자통도단백4(GIRK4)적표체.방법 30지SD대서안수궤수자표분량조,비반대서조(20지,고지고당음식구건비반대서모형)화정상대조조(10지,정상음식).건모성공처사량조대서후분리하구뇌、간、이등조직기관,제취단백,Western면역인적검측GIRK4표체.대비반대서조여정상대조조대서체질량、체장、지방계수등지표진행분석비교.결과 비반대서조대서체장、체질량、지방계수현저고우정상대조조[자:(22.00±0.71)비(20.80±0.45)cm,(289.10±29.06)비(239.60±10.08)g,(3.47±1.54)비(1.14±0.56)；웅:(26.32±0.87)비(24.80±0.84)em,(432.00±28.14)비(352.00±37.37)g,(3.20±0.56)비(1.11±0.27)；균P＜0.05],단하구뇌、간、이장적GIRK4표체각현저저우정상대조조(P＜0.05).결론 대서하구뇌、간장、이장교고표체적GIRK4가능여지방대사、음식유도형비반적발생유관.
Objective To investigate GIRK4 expression in hypothalamus,liver and pancreas of dietinduced obesity rat model.Methods Thirty SD rats were randomly assigned to obesity group (n=20) as fed by high-fat and high-sugar diet for establishment of obesity model and normal control group (n=10) as fed by normal diet.The rats were sacrificed followed by separation of organs (i.e.hypothalamus,liver and pancreas).Protein of above organs was extracted for detection of GIRK4 expression via Western immunoblotting.The weight,length and fat coefficient were compared.Results Obese rats yielded markedly higher weight[female:(289.10±29.06) vs (239.60±10.08)g,male:(432.OO±28.14) vs (352.00±37.37) g],length [female:(22.00±0.71) vs (20.80±0.45) cm,male:(26.32±0.87) vs (24.80±0.84)cm] and fat coefficient [female:(3.47±1.54) vs (1.14±0.56),male:(3.20±0.56) vs (1.11±0.27)] yet significantly suppressed GIRK4 expression in hypothalamus,liver and pancreas than normal controls (all P＜0.05).Conclusion The up-regulation of CIRK4 expression in hypothalamus,liver and pancreas may be related to fat metabolism and obesity induced by diet.
