CAJ | 학술논문

目的使用联合成像系统在亚细胞水平研究心肌细胞微区力学性质及细胞内钙离子的快速变化,并探讨两者之间的关系.方法应用原子力显微镜(AFM)和激光扫描共聚焦显微镜(LSCM)组合成的联合成像系统对急性分离的大鼠单个心室肌细胞微区力进行检测,同时测定细胞内钙离子浓度的变化,分析细胞微区力和细胞内钙离子浓度变化两者之间的相关性.结果观察到心肌细胞内钙离子浓度的快速变化引起了钙波的传递.通过AFM微悬臂的偏转可以计算出细胞微区力,细胞微区力的大小与细胞内钙离子浓度呈正相关(r=0.701,P=0.003).结论联合成像系统可以同步研究心肌细胞微区力学及细胞内分子快速变化,进而在亚细胞水平研究心脏疾病的发病机制.
목적 사용연합성상계통재아세포수평연구심기세포미구역학성질급세포내개리자적쾌속변화,병탐토량자지간적관계.방법 응용원자력현미경(AFM)화격광소묘공취초현미경(LSCM)조합성적연합성상계통대급성분리적대서단개심실기세포미구력진행검측,동시측정세포내개리자농도적변화,분석세포미구력화세포내개리자농도변화량자지간적상관성.결과 관찰도심기세포내개리자농도적쾌속변화인기료개파적전체.통과AFM미현비적편전가이계산출세포미구력,세포미구력적대소여세포내개리자농도정정상관(r=0.701,P=0.003).결론 연합성상계통가이동보연구심기세포미구역학급세포내분자쾌속변화,진이재아세포수평연구심장질병적발병궤제.
Objective To investigate the micro-mechanical properties of myocardial cell and rapid changes in intracellular calcium cation at the subcellular level with combination imaging system,and to explore the correlation between them above.Methods The micro-mechanics of acutely isolated single myocardial cells in rats were detected using the combination imaging system which was made up of atomic force rrucroscope (AFM) and laser scanning confocal microscope (LSCM),and the changes in intracellular calcium cation concentration were detected as well.The correlation between micro-mechanics of myocardial cell and changes in intracellular calcium cation was then analyzed.Results The rapid changes tn intracellular calcium cation concentration were found to cause the transmission of calcium waves.The strength of cell micro-region could be calculated by deflection of cantilever in AFM.And the strength of cell micro-region was positively correlated with intracellular calcium cation concentration (r=0.701,P=0.003).Conclusion The combination imaging system can simultaneously investigate the micro-mechanics of myocardial cell and rapid changes in intracellular molecule,and provide the technique for further research on the pathogenesis of heart disease at the subcellular level.