CAJ | 학술논문

目的研究组织型纤溶酶原激活剂(t-PA/PLAT)在移植血管桥再狭窄动物血管的差异表达.方法通过兔双侧颈动脉进行动脉桥和静脉桥的移植,形成双侧移植血管桥再狭窄动物模型.应用免疫组化检测t-PA在动物模型动脉桥、静脉桥的表达并进行比较.结果血管桥移植前,t-PA在实验动物颈动脉和颈静脉的表达差异无统计学意义(P＞0.05)；血管桥移植后,t-PA在动脉桥的表达明显高于静脉桥(P＜0.05),于16周时达到高峰[(32.34±4.74)％比(16.74±3.14)％],以后随时间延长而出现表达减少(P＜0.05).结论 t-PA在术后早期对血管桥具有保护作用,其表达的高低与术后血管桥再狭窄关系密切.
목적 연구조직형섬용매원격활제(t-PA/PLAT)재이식혈관교재협착동물혈관적차이표체.방법 통과토쌍측경동맥진행동맥교화정맥교적이식,형성쌍측이식혈관교재협착동물모형.응용면역조화검측t-PA재동물모형동맥교、정맥교적표체병진행비교.결과 혈관교이식전,t-PA재실험동물경동맥화경정맥적표체차이무통계학의의(P＞0.05)；혈관교이식후,t-PA재동맥교적표체명현고우정맥교(P＜0.05),우16주시체도고봉[(32.34±4.74)％비(16.74±3.14)％],이후수시간연장이출현표체감소(P＜0.05).결론 t-PA재술후조기대혈관교구유보호작용,기표체적고저여술후혈관교재협착관계밀절.
Objective To study the differential expression of tissue-type plasminogen activator (t-PA) in restenosis animal model following coronary artery bypass grafting (CABG).Methods The bilateral restenosis animal model following CABG was constructed by transplantation of the arterial and venous grafts to the carotid artery in rabbits.Assessment of the differential expression of t-PA in the arterial and venous grafts was carried out by immunohistochemistry assay.Results The difference in expression of t-PA in carotid artery and vein was unremarkable prior to vascular grafts (P＞0.05).The vascular graft surgery resulted in a significantly higher level of t-PA expression,which peaked at week 16 and reduced with time in the artery [(32.34±4.74)％ vs (16.74±3.14)％,P＜0.05],but not in the vein.Conclusion The t-PA may possess protective effects on vascular grafts at the early stage after vascular graft surgery,and is closely associated to the incidence of restenosis.