中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2013年
4期
300-305
,共6页
蛋白质组学%乙型肝炎病毒相关性肾炎%生物学标记%尿分析
蛋白質組學%乙型肝炎病毒相關性腎炎%生物學標記%尿分析
단백질조학%을형간염병독상관성신염%생물학표기%뇨분석
Proteomics%Hepatitis B virus associated-glomerulonephritis%Biological markers%Urinalysis
目的 研究乙型肝炎病毒相关性肾炎(HBV-GN)患者的尿液蛋白质谱特点,筛选差异表达的蛋白质.方法 用丙酮沉淀法提取HBV-GN患者(n=7)、原发性膜性肾病伴慢性乙型肝炎患者(n=5)、原发性膜性肾病患者(n=7)、慢性乙型肝炎无肾脏损害患者(n=7)及健康受试者(n=6)尿液标本中的蛋白质,酶解后行高效液相色谱-电喷雾离子阱串联质谱(HPLC-ESI-IT-MS/MS)检测.得到的肽序列结果用MASCOT软件行数据库检索,用查得的蛋白序列号在Uniprot数据库中进行检索,查询相关的蛋白质的信息.5组数据用手工方法进行组间对比,分析HBV-GN患者尿液中差异表达的蛋白质.结果 HBV-GN患者的尿液中检测出4种具有差异性表达的蛋白质,分别是α1抗胰凝乳蛋白酶、Igγ2链C区、前列腺素D2合酶、未命名蛋白(C9JAM6).结论 α1抗胰凝乳蛋白酶、前列腺素D2合酶有可能成为HBV-GN的生物标志物.
目的 研究乙型肝炎病毒相關性腎炎(HBV-GN)患者的尿液蛋白質譜特點,篩選差異錶達的蛋白質.方法 用丙酮沉澱法提取HBV-GN患者(n=7)、原髮性膜性腎病伴慢性乙型肝炎患者(n=5)、原髮性膜性腎病患者(n=7)、慢性乙型肝炎無腎髒損害患者(n=7)及健康受試者(n=6)尿液標本中的蛋白質,酶解後行高效液相色譜-電噴霧離子阱串聯質譜(HPLC-ESI-IT-MS/MS)檢測.得到的肽序列結果用MASCOT軟件行數據庫檢索,用查得的蛋白序列號在Uniprot數據庫中進行檢索,查詢相關的蛋白質的信息.5組數據用手工方法進行組間對比,分析HBV-GN患者尿液中差異錶達的蛋白質.結果 HBV-GN患者的尿液中檢測齣4種具有差異性錶達的蛋白質,分彆是α1抗胰凝乳蛋白酶、Igγ2鏈C區、前列腺素D2閤酶、未命名蛋白(C9JAM6).結論 α1抗胰凝乳蛋白酶、前列腺素D2閤酶有可能成為HBV-GN的生物標誌物.
목적 연구을형간염병독상관성신염(HBV-GN)환자적뇨액단백질보특점,사선차이표체적단백질.방법 용병동침정법제취HBV-GN환자(n=7)、원발성막성신병반만성을형간염환자(n=5)、원발성막성신병환자(n=7)、만성을형간염무신장손해환자(n=7)급건강수시자(n=6)뇨액표본중적단백질,매해후행고효액상색보-전분무리자정천련질보(HPLC-ESI-IT-MS/MS)검측.득도적태서렬결과용MASCOT연건행수거고검색,용사득적단백서렬호재Uniprot수거고중진행검색,사순상관적단백질적신식.5조수거용수공방법진행조간대비,분석HBV-GN환자뇨액중차이표체적단백질.결과 HBV-GN환자적뇨액중검측출4충구유차이성표체적단백질,분별시α1항이응유단백매、Igγ2련C구、전렬선소D2합매、미명명단백(C9JAM6).결론 α1항이응유단백매、전렬선소D2합매유가능성위HBV-GN적생물표지물.
Objective To analyze the characteristics of urine proteomics in patients with hepatitis B virus associated-glomerulonephritis (HBV-GN) for screening the differentially expressed proteins.Methods The urinary proteins were precipitated with acetone in 7 cases with HBV-GN,5 with primary membranous nephropathy complicated with chronic hepatitis B,7 with primary membranous nephropathy,7 with chronic hepatitis B without renal damage and 6 healthy subjects.This entailed enzyme cleavage followed by highperformance liquid chromatography-electrospray ionization-ion trap tandem mass spectroscopy (HPLC-ESI-IT-MS/MS) for deriving the peptide sequences that were subsequently retrieved from the database by using MASCOT software.This was followed by protein sequence searching based on the Uniprot database.The among-group difference was compared by manual calculation to analyze the differentially expressed proteins in the urine of HBVGN patients.Results A total of 4 differentially expressed proteins,namely alpha-1-antichymotrypsin,Ig gamma2 chain C region,prostaglandin D2 synthase and C9JAM6 (an unnamed protein),were identified in the urine of HBV-GN patients.Conclusion Alpha-1-antichymotrypsin and prostaglandin D2 synthase may be the potential biomarkers of HBV-GN.
