CAJ | 학술논문

目的探讨检测自身免疫性脑病(AE)患儿外周血中γδT细胞的临床意义.方法应用流式细胞仪对36例AE患儿和23例健康儿童外周血中γδT细胞、CD3+淋巴细胞以及CD4+/CD8+比值进行检测;同时分析AE患儿治疗前后的上述细胞变化情况.结果 AE患儿外周血γδT细胞和CD3+淋巴细胞分别为17.54％±2.65％、66.57％±5.01％,显著高于健康对照组(6.47％±2.39％,P＜0.01,61.49％±3.87％,P＜0.05);而CD4+/CD8+比值为1.05±0.27,显著低于健康对照组(1.43±0.28,P＜0.05).AE患儿治疗后γδT细胞为9.12％±3.24％,较治疗前17.54％±2.65％明显降低,差异有统计学意义(t=10.467,P＜0.01).结论 AE患儿外周血γδT细胞比例变化与病情有关,γδT细胞检测可作为AE治疗的一项监测指标.
목적 탐토검측자신면역성뇌병(AE)환인외주혈중γδT세포적림상의의.방법 응용류식세포의대36례AE환인화23례건강인동외주혈중γδT세포、CD3+림파세포이급CD4+/CD8+비치진행검측;동시분석AE환인치료전후적상술세포변화정황.결과 AE환인외주혈γδT세포화CD3+림파세포분별위17.54％±2.65％、66.57％±5.01％,현저고우건강대조조(6.47％±2.39％,P＜0.01,61.49％±3.87％,P＜0.05);이CD4+/CD8+비치위1.05±0.27,현저저우건강대조조(1.43±0.28,P＜0.05).AE환인치료후γδT세포위9.12％±3.24％,교치료전17.54％±2.65％명현강저,차이유통계학의의(t=10.467,P＜0.01).결론 AE환인외주혈γδT세포비례변화여병정유관,γδT세포검측가작위AE치료적일항감측지표.
Objective To investigate the clinical significance of peripheral blood γδT cells detection in children with autoimmune encephalopathy (AE).Methods Peripheral blood γδT cells,CD3+ lymphocytes and CD4+/CD8 + were detected by flow cytometry in 36 AE children and 23 healthy children controls.Lever of γδT cells in AE children was compared before and after treatment.Results AE children showed significantly higher levels of γδT cells (17.54％ ± 2.65％ vs 6.47％ ± 2.39％,P＜0.01) and CD3 + lymphocytes (66.57％±5.01％ vs 61.49％±3.87％,P＜0.05) and lower CD4+/CD8+ ratio (1.05±0.27 vs 1.43± 0.28,P＜0.05) as compared with healthy controls.After treatment,the level of γδT cells (9.12％±3.24％ vs 17.54％±2.65％,P＜0.01) in AE children was decreased from baseline with statistical difference (t=10.467,P＜0.01).Conclusions The lever of γδT cells is associated to autoimmune encephalopathy.Detection of γδT cells can be used as an biomarker for treatment of AE.