中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2013年
6期
433-437
,共5页
李倩君%王丙剑%郑翔翔%郭慧敏%周传文%任建国%潘峰
李倩君%王丙劍%鄭翔翔%郭慧敏%週傳文%任建國%潘峰
리천군%왕병검%정상상%곽혜민%주전문%임건국%반봉
胃肿瘤%RNA干扰%E-钙黏蛋白%Snai1%肿瘤侵润
胃腫瘤%RNA榦擾%E-鈣黏蛋白%Snai1%腫瘤侵潤
위종류%RNA간우%E-개점단백%Snai1%종류침윤
Stomach neoplasms%RNA interference%E-cadherin%Snai1%Neoplasm invasiveness
目的 探讨锌指转录因子Snai1在胃癌发生发展中的作用,以及胃癌组织中Snai1与E-钙黏蛋白(E-cadherin)表达的关系.方法 收集本院普外科2010年1月至2012年1月收治的65例胃癌患者手术切除的胃癌组织及邻近的正常胃黏膜组织,免疫组织化学检测Snai1和E-cadherin的表达.体外培养人胃腺癌细胞株SGC-7901,分为3组:空白对照组不进行转染,阴性对照组转染不含靶向作用于Snai1的siRNA的空质粒,转染组转染靶向作用于Snai1的siRNA.转染结束后实时定量PCR和免疫印迹法检测3组细胞Snai1和E-cadherin的表达.结果 胃癌组织中Snai1表达阳性率较正常胃黏膜组织明显增高(58.5％比0.0％,P＜0.001),而E-cadherin表达阳性率明显降低(43.1％比100.0％,P＜0.001).Snai1阳性和阴性的胃癌组织中E-cadherin表达的阳性率分别为28.9％ (11/38)、63.0％(17/27).Snai1阳性的胃癌患者较Snai1阴性的胃癌患者淋巴结转移率明显升高(78.9％比40.7％,P＜0.001).体外细胞实验显示,空白对照组与阴性对照组比较,Snai1和E-cadherin的表达差异无统计学意义(均P＞0.05)；与空白对照组比较,转染组Snai1 mRNA和蛋白表达下调,而E-cadherin mRNA和蛋白表达上调(均P＜0.05).结论 Snai1可通过抑制E-cadherin表达,促进胃癌发生局部浸润及淋巴结转移.
目的 探討鋅指轉錄因子Snai1在胃癌髮生髮展中的作用,以及胃癌組織中Snai1與E-鈣黏蛋白(E-cadherin)錶達的關繫.方法 收集本院普外科2010年1月至2012年1月收治的65例胃癌患者手術切除的胃癌組織及鄰近的正常胃黏膜組織,免疫組織化學檢測Snai1和E-cadherin的錶達.體外培養人胃腺癌細胞株SGC-7901,分為3組:空白對照組不進行轉染,陰性對照組轉染不含靶嚮作用于Snai1的siRNA的空質粒,轉染組轉染靶嚮作用于Snai1的siRNA.轉染結束後實時定量PCR和免疫印跡法檢測3組細胞Snai1和E-cadherin的錶達.結果 胃癌組織中Snai1錶達暘性率較正常胃黏膜組織明顯增高(58.5％比0.0％,P＜0.001),而E-cadherin錶達暘性率明顯降低(43.1％比100.0％,P＜0.001).Snai1暘性和陰性的胃癌組織中E-cadherin錶達的暘性率分彆為28.9％ (11/38)、63.0％(17/27).Snai1暘性的胃癌患者較Snai1陰性的胃癌患者淋巴結轉移率明顯升高(78.9％比40.7％,P＜0.001).體外細胞實驗顯示,空白對照組與陰性對照組比較,Snai1和E-cadherin的錶達差異無統計學意義(均P＞0.05)；與空白對照組比較,轉染組Snai1 mRNA和蛋白錶達下調,而E-cadherin mRNA和蛋白錶達上調(均P＜0.05).結論 Snai1可通過抑製E-cadherin錶達,促進胃癌髮生跼部浸潤及淋巴結轉移.
목적 탐토자지전록인자Snai1재위암발생발전중적작용,이급위암조직중Snai1여E-개점단백(E-cadherin)표체적관계.방법 수집본원보외과2010년1월지2012년1월수치적65례위암환자수술절제적위암조직급린근적정상위점막조직,면역조직화학검측Snai1화E-cadherin적표체.체외배양인위선암세포주SGC-7901,분위3조:공백대조조불진행전염,음성대조조전염불함파향작용우Snai1적siRNA적공질립,전염조전염파향작용우Snai1적siRNA.전염결속후실시정량PCR화면역인적법검측3조세포Snai1화E-cadherin적표체.결과 위암조직중Snai1표체양성솔교정상위점막조직명현증고(58.5％비0.0％,P＜0.001),이E-cadherin표체양성솔명현강저(43.1％비100.0％,P＜0.001).Snai1양성화음성적위암조직중E-cadherin표체적양성솔분별위28.9％ (11/38)、63.0％(17/27).Snai1양성적위암환자교Snai1음성적위암환자림파결전이솔명현승고(78.9％비40.7％,P＜0.001).체외세포실험현시,공백대조조여음성대조조비교,Snai1화E-cadherin적표체차이무통계학의의(균P＞0.05)；여공백대조조비교,전염조Snai1 mRNA화단백표체하조,이E-cadherin mRNA화단백표체상조(균P＜0.05).결론 Snai1가통과억제E-cadherin표체,촉진위암발생국부침윤급림파결전이.
Objective To investigate the role of Snai1,the zinc finger transcriptional factor,in the pathogenesis of gastric carcinoma,and to examine the relationship between Snai 1 and E-cadherin expression.Methods The specimens of primary gastric carcinoma and adjacent normal gastric mucosa derived from 65 patients with gastric carcinoma who underwent gastrectomy between January 2010 and January 2012 in the department of gastroenterology of our hospital were subjected to immunohistochemistry assay for the measurement of Snai1 and E-cadherin expression.By using real-time polymerase chain reaction and Western blotting,the expression of Snai1 and E-cadherin in SGC-7901,a gastric cancer cell line,incubated in vitro,was quantified.This cell line was assigned to blank control group (untransfected with plasmids),negative control group (transfected with blank siRNA vector without Snai1 gene sequence) and SGC-7901-siRNA group (transfected with siRNA vector),respectively.Results Gastric carcinoma tissues yielded markedly higher Snai 1 expression (58.5％ vs 0.0％,P＜0.001) yet significantly lower E-cadherin expression (43.1％ vs 100.0％,P＜0.O01) than normal gastric mucosa.The positivity of E-cadherin was 28.9％ (11/38) and 63.0％ (12/27) in Snai1-positive and-negative specimens.The rate of lymph node metastasis was markedly higher in Snai1-positive than that in Snai1-negative cases (78.9％ vs 40.7％,P＜0.001).In vitro cell experiments suggested an unremarkable difference between Snai 1 and E-cadherin expression between blank control group and negative control group (P＞0.05).Compared with blank control group,the SGC-7901-siRNA group was characterized by pronouncedly attenuated Snai1 mRNA and protein expression and considerably up-regulated E-cadherin mRNA and protein expression (all P＜0.05).Conclusion Snai1 promotes focal infiltration and lymph node metastasis of gastric carcinoma via inhibition of E-cadherin expression .
