CAJ | 학술논문

目的探讨基因型分析法检测结核分枝杆菌异烟肼和利福平耐药性的价值.方法采用多重PCR和线性探针反向膜杂交法来检测异烟肼耐药基因katG S315T和inhA C-15T突变以及利福平耐药基因rpoB D516V,H526Y,H526D,S531L突变来判断78株结核分枝杆菌临床分离株的耐药性,并与金标准L-J固体培养基药敏法以及BACTEC960液体培养药敏法进行对比分析.结果基因型分析法在6h内可以完成;结核分枝杆菌常规药敏检测需要3个月.与后者相比,基因型分析法检测异烟肼耐药的敏感性和特异性分别为89％ (16/18)和99％ (77/78);检测利福平耐药的敏感性和特异性均为100％(13/13,78/78).结论基因型分析法检测结核菌耐药性快速准确,对耐药结核病的早期诊断和治疗很有帮助,有望在临床实验室广泛开展.
목적 탐토기인형분석법검측결핵분지간균이연정화리복평내약성적개치.방법 채용다중PCR화선성탐침반향막잡교법래검측이연정내약기인katG S315T화inhA C-15T돌변이급리복평내약기인rpoB D516V,H526Y,H526D,S531L돌변래판단78주결핵분지간균림상분리주적내약성,병여금표준L-J고체배양기약민법이급BACTEC960액체배양약민법진행대비분석.결과 기인형분석법재6h내가이완성;결핵분지간균상규약민검측수요3개월.여후자상비,기인형분석법검측이연정내약적민감성화특이성분별위89％ (16/18)화99％ (77/78);검측리복평내약적민감성화특이성균위100％(13/13,78/78).결론 기인형분석법검측결핵균내약성쾌속준학,대내약결핵병적조기진단화치료흔유방조,유망재림상실험실엄범개전.
Objective To explore the value of the genotype assays detection of the resistance of Mycobacterium tuberculosis to isoniazid and rifampin.Methods Seventy-eight clinical isolates susceptible of harboring Mycobacterium tuberculosis were subject to the mutation assay of katG gene S315T,inhA gene C-15Tand rpoB genes D516V,H526Y,H526D,S531L by using multiplex polymerase chain reaction-based linear probe membrane hybridization for analysis of isoniazid and rifampin resistance.These results were compared with the L-J solid medium culture,the gold standard,and MGIT BACTEC 960 drug sensitivity test.Results Genotype assay was associated with shorter time for the detection compared with routine drug sensitivity test (6 hours vs 3 months).The genotype assay yielded the sensitivity and specificity of 89％ (16/18) and 99％ (77/78) for isoniazid and 100％ and 100％ (13/13 and 78/78) for rifampin.Conclusion Genotype assay of Mycobacterium tuberculosis offers a rapid and accurate approach for the early diagnosis and treatment of drug-resistant tuberculosis,rendering it a promising technique to be extensively applied in clinical laboratories.