CAJ | 학술논문

目的检测在一定水平的脉冲电场刺激的作用下,细胞在支架上的黏附、生长情况,并与传统静态细胞培养下的细胞作比较,从而探究脉冲电场刺激对细胞的生长率、黏附率所产生的影响.方法构建聚己内酯(PCL)纳米纤维支架,将人内皮祖细胞接种于已缝有PCL的电刺激反应器上,按照0V、1V、2V、4V的电压分组,分别反应1h、2h,采用HE切片、电镜、MTT等方法,观察细胞在PCL上的黏附情况及细胞活力.结果通过脉冲电场刺激的方法,细胞黏附效率得以进一步提高,与传统静态培养下的细胞相比,经过脉冲电场刺激的细胞更易在PCL支架表面黏附与生长,而50 Hz,2V/cm的条件即是诱导细胞黏附的最佳环境条件.结论脉冲电场刺激的应用在组织工程学方面具备良好的发展潜力.
목적 검측재일정수평적맥충전장자격적작용하,세포재지가상적점부、생장정황,병여전통정태세포배양하적세포작비교,종이탐구맥충전장자격대세포적생장솔、점부솔소산생적영향.방법 구건취기내지(PCL)납미섬유지가,장인내피조세포접충우이봉유PCL적전자격반응기상,안조0V、1V、2V、4V적전압분조,분별반응1h、2h,채용HE절편、전경、MTT등방법,관찰세포재PCL상적점부정황급세포활력.결과 통과맥충전장자격적방법,세포점부효솔득이진일보제고,여전통정태배양하적세포상비,경과맥충전장자격적세포경역재PCL지가표면점부여생장,이50 Hz,2V/cm적조건즉시유도세포점부적최가배경조건.결론 맥충전장자격적응용재조직공정학방면구비량호적발전잠력.
Objective To explore the cell adhesion and growth on the scaffold under a certain magnitude of electric field,and to compare with the cells incubated using conventional static culture techniques.Methods A synthetic polycaprolactone (PCL) nanofiberous scaffold for incubation of the endothelial progenitor cellswas constructed and was sewn on the electrical stimulator.This entailed the allocation of the cells to four groups according to the voltage (0 V,1 V,2 V and 4 V).Haematoxylin-eosin staining,transmission electron microscopy and MTF technique were applied to determine the cell adhesion and viability.Results The electric fields improved cell adhesion on the PCL scaffolds,with the optimal condition being the stimulation of 50 Hz and 2 V/cm.Conclusion The pulsatile electric field may be a promising approach for vascular tissue engineering.