CAJ | 학술논문

目的比较采用自制脂肪乳剂灌胃和普通高脂饲料喂养两种方法建立家兔高脂血症动物模型的效果.方法 40只家兔按随机数字表分为自制脂肪乳剂灌胃组(n=20)和高脂饲料喂养组(n=20),分别给予自制脂肪乳剂灌胃连续2周和高脂饲料喂养连续4周.所有动物分别在实验前、后测定血清总胆固醇(TC)、三酰甘油(TG)、载脂蛋白A1 (ApoAl)、ApoB,血浆血栓素B2(TXB2)、6-酮-前列腺素F1α(6-keto-PGF1α)、内皮素(ET)、红细胞膜总胆醇(M-TC)、红细胞膜流动性(M-Flu)、红细胞丙二醛(E-MDA)、红细胞超氧化物歧化酶(E-SOD)水平,实验结束时取主动脉组织进行病理检查.结果实验后两组家兔TC、TG、ApoB、TXB2、ET、E-MDA水平较实验前升高,ApoA1、6-keto-PGF1α、M-Flu、E-SOD水平较实验前降低(均P＜0.05);而M-TC水平差异无统计学意义(均P＞0.05).实验前、后两组同一指标组间比较,差异均无统计学意义(均P＞0.05).病理检查显示两组动物主动脉组织均出现动脉粥样硬化早期改变.结论两种方法均可以成功建立高脂血症动物模型,采用自制脂肪乳剂灌胃建立高脂血症动物模型的方法具有可行性.
목적 비교채용자제지방유제관위화보통고지사료위양량충방법건립가토고지혈증동물모형적효과.방법 40지가토안수궤수자표분위자제지방유제관위조(n=20)화고지사료위양조(n=20),분별급여자제지방유제관위련속2주화고지사료위양련속4주.소유동물분별재실험전、후측정혈청총담고순(TC)、삼선감유(TG)、재지단백A1 (ApoAl)、ApoB,혈장혈전소B2(TXB2)、6-동-전렬선소F1α(6-keto-PGF1α)、내피소(ET)、홍세포막총담순(M-TC)、홍세포막류동성(M-Flu)、홍세포병이철(E-MDA)、홍세포초양화물기화매(E-SOD)수평,실험결속시취주동맥조직진행병리검사.결과 실험후량조가토TC、TG、ApoB、TXB2、ET、E-MDA수평교실험전승고,ApoA1、6-keto-PGF1α、M-Flu、E-SOD수평교실험전강저(균P＜0.05);이M-TC수평차이무통계학의의(균P＞0.05).실험전、후량조동일지표조간비교,차이균무통계학의의(균P＞0.05).병리검사현시량조동물주동맥조직균출현동맥죽양경화조기개변.결론 량충방법균가이성공건립고지혈증동물모형,채용자제지방유제관위건립고지혈증동물모형적방법구유가행성.
Objective To compare the outcomes of self-made intralipid gastric perfusion with normal high cholesterol feeding in developing hyperlipidemia rabbit models.Methods Forty rabbits were randomly divided into self-made intralipid gastric perfusion group (n=20) and normal high cholesterol feeding group (n=20) based on the random digit table.The rabbits were given self-made intralipid gastric perfusion for 2 weeks and high cholesterol food for 4 weeks respectively.The levels of serum total cholesterol (TC),triglyceride (TG),apolipoprotein A1 (ApoA1),ApoB,plasma thromboxane B2 (TXB2),6-keto-prostaglandin F1α (6-keto-PGF1α),endotheline (ET),erythrocyte membrane total cholesterol (M-TC),erythrocyte membrane fluidness (M-Flu),erythrocyte malondialdehyde (E-MDA) and erythrocyte superoxide dismutase (E-SOD) were measured before and after the experiment in all rabbits.The aorta was taken to undergo pathological examination after experiment.Results Compared with baseline,the levels of TC,TG,ApoB,TXB2,ET and E-MDA in two groups of rabbits were increased after the experiment,meanwhile,ApoA1,6-keto-PGF1α,M-Flu,E-SOD were decreased (all P＜0.05),but there were no statistical difference in M-TC level of two groups (bothP＞0.05).There were no statistical difference in any given parameters between two groups before and after the experiment (all P＞0.05). Pathological examination suggested early atherosclerosis of the aorta in both groups.Conclusions Hyperlipidemia animal models can be successfully established by both ways,suggesting the feasibility to use self-made intralipid gastric perfusion to develop hyperlipidemia animal models.