CAJ | 학술논문

目的观探讨川芎嗪在自体血回输对保护细胞免疫功能的抗氧化损伤作用.方法 60例择期行脊柱手术患者,ASA分级Ⅰ～Ⅱ级,术前评估出血量大于400 ml,完全随机分两组:试验组和对照组(每组30例).试验组于收集血液前30 min经静脉滴注川芎嗪2 mg/kg,在回收血液的肝素盐水和洗涤盐水内加入川芎嗪,终浓度为0.005％;对照组不予静滴川芎嗪,肝素盐水和洗涤盐水内不加川芎嗪.记录术中出血量及回输血量,采集术前(T0)及回输血后1h、1d、5d(T1、T2、T3)的静脉血,测定超氧化物歧化酶(SOD)活性和丙二醛(MDA)浓度的变化.结果与To比较,两组患者T1、T2时SOD活性均降低,MDA浓度均升高,T3时试验组SOD比术前高,对照组仍比术前低;试验组MDA基本恢复至术前水平,对照组仍比术前高.组间比较两组SOD、MDA在各时点均差异有统计学意义(P＜0.05).结论川芎嗪在自体血回输的应用中具有的抗氧化损伤作用.
목적 관탐토천궁진재자체혈회수대보호세포면역공능적항양화손상작용.방법 60례택기행척주수술환자,ASA분급Ⅰ～Ⅱ급,술전평고출혈량대우400 ml,완전수궤분량조:시험조화대조조(매조30례).시험조우수집혈액전30 min경정맥적주천궁진2 mg/kg,재회수혈액적간소염수화세조염수내가입천궁진,종농도위0.005％;대조조불여정적천궁진,간소염수화세조염수내불가천궁진.기록술중출혈량급회수혈량,채집술전(T0)급회수혈후1h、1d、5d(T1、T2、T3)적정맥혈,측정초양화물기화매(SOD)활성화병이철(MDA)농도적변화.결과 여To비교,량조환자T1、T2시SOD활성균강저,MDA농도균승고,T3시시험조SOD비술전고,대조조잉비술전저;시험조MDA기본회복지술전수평,대조조잉비술전고.조간비교량조SOD、MDA재각시점균차이유통계학의의(P＜0.05).결론 천궁진재자체혈회수적응용중구유적항양화손상작용.
Objective To investigate the action of ligustrazine against oxidative damage for cellular immunoprotection in autologous blood transfusion.Methods Sixty patients scheduled for selective lumbar surgery (ASA Ⅰ to Ⅱ),with a pre-surgical estimation of blood loss ＞ 400 ml,were recruited.They were randomized into two groups,the study group and the control group (n=30 each).In the study group,Ligustrazine was given to patients by intravenous dripping at a dose of 2 mg/kg 30 mins before blood collection,and also added to the washing saline and heparinized saline solution (final concentration:0.005％) for processing of the collected blood.No Ligustrazine was given to patients or added to the washing saline and heparinized saline solution in the control group.The amount of blood loss and auto-transfusion were measured and recorded.Venous blood samples were collected from all patients before surgery (To) and at 1 hour,1 and 5 days after (T1,T2 and T3) and measured for the change in SOD activity and MDA level.Results In both groups,the SOD activity was significantly lower and MDA concentration was significantly higher at T1 and T2 as compared to the baseline values at To At T3 SOD activity in the study group was higher but remained a low level in the control group as compared with preoperation,while the MDA in the study group returned to preoperative level but was still higher than baseline in the control group.The SOD activity and MDA level were significantly different between two groups at all time points.(P＜0.05).Conclusion Ligustrazine has a role against oxidative damage in autotransfusion.