CAJ | 학술논문

目的探讨盐酸戊乙奎醚(PHC)对粘连性肠梗阻(AIO)大鼠肠黏膜免疫屏障功能的影响及可能机制.方法将60只SD大鼠分为正常组(N组)、假手术组(S组)、生理盐水组(NS组)及盐酸戊乙奎醚组(P2、P4、P8组).建立AIO模型.正常组不予处理,生理盐水组及假手术组均给予0.4 ml生理盐水,PHC组按0.2、0.4、0.8 mg/kg配成0.4 ml,于术后第7d开始连续肌注7d.ELISA法测量血清中IL-4、IL-10含量以及肠内容物中分泌型免疫球蛋白A(sIgA)含量.结果与S组、NS组比较,P2、P4、P8组血清IL-4[(19.34±2.87),(23.85±2.72),(27.89±2.71) vs (6.18±1.42),(8.37±2.69) ng/L)]、IL-10[(118.93±20.78),(121.61±24.932),(127.41±22.61) vs (78.19±19.41),(42.72±21.31) ng/L]以及肠内容物中sIgA含量(0.64±0.089,0.99±0.093,1.02±0.12 vs 0.41±0.06,0.14±0.03 ng/L)均有明显增高,差异均有统计学意义(均P＜ 0.05).与P2组比较,P4、P8组IL-4与sIgA水平升高更为明显,差异均有统计学意义(P＜ 0.05).结论中、高剂量PHC对AIO大鼠肠黏膜的免疫屏障有一定保护作用,且以高剂量更为显著,其机制可能与增加抗炎因子释放和抑制炎性因子产生有关.
목적 탐토염산무을규미(PHC)대점련성장경조(AIO)대서장점막면역병장공능적영향급가능궤제.방법 장60지SD대서분위정상조(N조)、가수술조(S조)、생리염수조(NS조)급염산무을규미조(P2、P4、P8조).건립AIO모형.정상조불여처리,생리염수조급가수술조균급여0.4 ml생리염수,PHC조안0.2、0.4、0.8 mg/kg배성0.4 ml,우술후제7d개시련속기주7d.ELISA법측량혈청중IL-4、IL-10함량이급장내용물중분비형면역구단백A(sIgA)함량.결과 여S조、NS조비교,P2、P4、P8조혈청IL-4[(19.34±2.87),(23.85±2.72),(27.89±2.71) vs (6.18±1.42),(8.37±2.69) ng/L)]、IL-10[(118.93±20.78),(121.61±24.932),(127.41±22.61) vs (78.19±19.41),(42.72±21.31) ng/L]이급장내용물중sIgA함량(0.64±0.089,0.99±0.093,1.02±0.12 vs 0.41±0.06,0.14±0.03 ng/L)균유명현증고,차이균유통계학의의(균P＜ 0.05).여P2조비교,P4、P8조IL-4여sIgA수평승고경위명현,차이균유통계학의의(P＜ 0.05).결론 중、고제량PHC대AIO대서장점막적면역병장유일정보호작용,차이고제량경위현저,기궤제가능여증가항염인자석방화억제염성인자산생유관.
Objective To investigate the effect and possible mechanism of penehyclidine hydrochloride (PHC) on the immune barrier of the intestinal mucosa in rats with adhesive intestinal obstruction (AIO).Methods Sixty Sprague-Dawley (SD) rats were divided into the normal group (N group),sham-operated group (S group),normal saline group (NS group) and penehyclidine hydrochloride groups (P2,P4,and P8 groups).AIO model was established.The N group did not receive any treatment,while the Group NS and Group S received intramuscular injection of 0.4 ml normal saline,and PHC groups received intramuscular injection of 0.4 ml PHC (dosing to 0.2,0.4 and 0.8 mg/kg for Groups P2,P4 and P8 groups,respectively) for 7 days starting from day 7 after the modeling procedure.ELISA was used to measure the serum levels of IL-4 and IL-10,and the level of secretory immunoglobulin A (sIgA) in intestinal contents.Results Compared with S and NS groups,P2,P4,and P8 groups had higher serum IL-4 [(19.34±2.87),(23.85±2.72),(27.89±2.71) vs (6.18±1.42),(8.37±2.69) ng/L)],IL-10 [(118.93± 20.78),(121.61 ±24.932),(127.41 ±22.61) vs (78.19± 19.41),(42.72±21.31) ng/L] and sIgA of intestinal contents As compared to P2 groups,P4 and P8 groups had higher.IL-4 and sIgA(All P ＜ 0.05).Conclusion Moderate to high dosage of PHC can protect the immune barrier of the intestinal mucosa from injury after AIO in rats.Such protection may be related to the increased release of anti-inflammatory cytokines and suppressed production of inflammatory cytokines.