中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2014年
4期
294-297
,共4页
乳果糖%微核试验%环磷酰胺%富氢水%突变
乳果糖%微覈試驗%環燐酰胺%富氫水%突變
유과당%미핵시험%배린선알%부경수%돌변
Lactulose%Micronucleus tests%Cyclophosphamide%Hydrogen-rich water%Mutation
目的 探讨饮用富氢水和产氢营养因子乳果糖的抗突变作用.方法 36只昆明小鼠按随机数字表分为6组,每组6只.空白对照组(A组)、富氢水处理组(B组)和乳果糖处理组(C组):这3组小鼠正常喂养3d后,第4～6天分别使用蒸馏水、富氢水、50％乳果糖灌胃(20 ml/kg、1次/d);抗生素对照组(D组)、抗生素+富氢水处理组(E组)和抗生素+乳果糖处理组(F组):这3组小鼠第1～3天随意饮用含抗生素的水,第4～6天分别使用蒸馏水、富氢水、50％乳果糖灌胃(20 ml/kg、1次/d).末次灌胃后小鼠腹腔注射环磷酰胺(0.04 g/kg),24 h后处死小鼠,进行骨髓细胞学检查,计算各组小鼠骨髓嗜多染红细胞微核率.结果 A、B、C、D、E、F组小鼠骨髓嗜多染红细胞微核率分别为(16.00±0.67)‰、(15.11±0.25)‰、(10.17±0.35)‰、(22.39±0.51)‰、(15.44±0.44)‰、(18.67±0.37)‰.与A组比较,C组微核率显著减少,D组微核率显著增加(均P＜0.01).与D组比较,E组和F组微核率显著减少(均P＜o.o1).与C组比较,F组微核率显著增加(P＜0.01).结论 富氢水和乳果糖均可拮抗环磷酰胺的致突变作用,抗生素预处理可以显著降低乳果糖的抗突变作用.
目的 探討飲用富氫水和產氫營養因子乳果糖的抗突變作用.方法 36隻昆明小鼠按隨機數字錶分為6組,每組6隻.空白對照組(A組)、富氫水處理組(B組)和乳果糖處理組(C組):這3組小鼠正常餵養3d後,第4～6天分彆使用蒸餾水、富氫水、50％乳果糖灌胃(20 ml/kg、1次/d);抗生素對照組(D組)、抗生素+富氫水處理組(E組)和抗生素+乳果糖處理組(F組):這3組小鼠第1～3天隨意飲用含抗生素的水,第4～6天分彆使用蒸餾水、富氫水、50％乳果糖灌胃(20 ml/kg、1次/d).末次灌胃後小鼠腹腔註射環燐酰胺(0.04 g/kg),24 h後處死小鼠,進行骨髓細胞學檢查,計算各組小鼠骨髓嗜多染紅細胞微覈率.結果 A、B、C、D、E、F組小鼠骨髓嗜多染紅細胞微覈率分彆為(16.00±0.67)‰、(15.11±0.25)‰、(10.17±0.35)‰、(22.39±0.51)‰、(15.44±0.44)‰、(18.67±0.37)‰.與A組比較,C組微覈率顯著減少,D組微覈率顯著增加(均P＜0.01).與D組比較,E組和F組微覈率顯著減少(均P＜o.o1).與C組比較,F組微覈率顯著增加(P＜0.01).結論 富氫水和乳果糖均可拮抗環燐酰胺的緻突變作用,抗生素預處理可以顯著降低乳果糖的抗突變作用.
목적 탐토음용부경수화산경영양인자유과당적항돌변작용.방법 36지곤명소서안수궤수자표분위6조,매조6지.공백대조조(A조)、부경수처리조(B조)화유과당처리조(C조):저3조소서정상위양3d후,제4～6천분별사용증류수、부경수、50％유과당관위(20 ml/kg、1차/d);항생소대조조(D조)、항생소+부경수처리조(E조)화항생소+유과당처리조(F조):저3조소서제1～3천수의음용함항생소적수,제4～6천분별사용증류수、부경수、50％유과당관위(20 ml/kg、1차/d).말차관위후소서복강주사배린선알(0.04 g/kg),24 h후처사소서,진행골수세포학검사,계산각조소서골수기다염홍세포미핵솔.결과 A、B、C、D、E、F조소서골수기다염홍세포미핵솔분별위(16.00±0.67)‰、(15.11±0.25)‰、(10.17±0.35)‰、(22.39±0.51)‰、(15.44±0.44)‰、(18.67±0.37)‰.여A조비교,C조미핵솔현저감소,D조미핵솔현저증가(균P＜0.01).여D조비교,E조화F조미핵솔현저감소(균P＜o.o1).여C조비교,F조미핵솔현저증가(P＜0.01).결론 부경수화유과당균가길항배린선알적치돌변작용,항생소예처리가이현저강저유과당적항돌변작용.
Objective To evaluate the anti-mutagenic effect of potable hydrogen-rich water and lactulose,a hydrogen-producing prebiotics.Methods Thirty-six Kungming mice were randomly divided into 6 groups (n=6 each) by random number table.Blank control group (group A),hydrogen-rich water treatment group (group B),and lactulose treatment group (group C),after 3 days of normal feeding,these 3 groups received gavage of distilled water,hydrogen-rich water and 50％ lactulose (20 ml/kg,once-daily),respectively from days 4 to 6.Another 3 groups were antibiotic control group (group D),antibiotic + hydrogen-rich water treatment group (group E) and antibiotic + lactulose treatment group (group F),mice of these 3 groups had free access to antibiotic-containing water from days 1 to 3,then received garage of distilled water,hydrogen-rich water and 50％ lactulose (20 ml/kg,once-daily),respectively from days 4 to 6.After the last gavage,the mice were intraperitoneally injected with cyclophosphamide (0.04 g/kg),and executed at 24 h later.The bone marrow cells were detected and micronuclei rate of polychromatic erythrocytes of mice bone marrow in each group was calculated.Results The micronuclei rate of polychromatic erythrocytes in groups A,B,C,D,E and F was (16.00±0.67)‰,(15.11±0.25)‰,(10.17±0.35)‰,(2239±0.51)‰,(15.44±0.44)‰,and (18.67±0.37)‰ respectively.Compared with group A,the micronuelei rate was significantly lower in group C,but significantly higher in group D (both P＜0.01).Compared with group D,the micronuclei rates in groups E and F were significantly lower (both P＜0.01).Compared with Group C,the micronuclei rate in group F was significantly elevated (P＜0.01).Conclusion Both hydrogen-rich water and lactulose have antimutagenic effect against cyclophosphamide,and antibiotic pretreatment can significantly reduce the antimutagenic effect of lactulose.
