中国小儿急救医学
中國小兒急救醫學
중국소인급구의학
CHINESE PEDIATRIC EMERGENCY MEDICINE
2014年
3期
129-133
,共5页
林广裕%蔡晓莹%蔡志伟%林创兴%陈派镇%周晓华%肖泽曦%陆学东
林廣裕%蔡曉瑩%蔡誌偉%林創興%陳派鎮%週曉華%肖澤晞%陸學東
림엄유%채효형%채지위%림창흥%진파진%주효화%초택희%륙학동
新型病毒%呼吸道病毒%儿科重症监护室
新型病毒%呼吸道病毒%兒科重癥鑑護室
신형병독%호흡도병독%인과중증감호실
Emerging virus%Respiratory virus%Pediatric intensive care unit
目的 了解新型呼吸道病毒在PICU中的检出情况及感染患儿的临床特点.方法 收集我院600例PICU急性呼吸道感染患儿的咽拭子标本600份,87例社区健康体检儿童咽拭子87份作为健康对照.应用多重PCR技术对新型呼吸道病毒进行检测,部分阳性结果进行基因测序,并分析阳性病例的临床资料.结果 (1) 600例患儿中,新型呼吸道病毒检测阳性114例(19％),其中人博卡病毒(human bocavirus,HBoV)48例(8.00％),C型鼻病毒(human rhinovirus group C,HRV-C)47例(7.83％),新型甲型流感H1N1病毒13例(2.17％),人偏肺病毒(human metapneumovirs,HMPV)10例(1.67％),WU多瘤病毒3例(0.50％).87例健康儿童呼吸道病毒检测均为阴性.(2) 114例新型呼吸道病毒检测阳性患儿中,单一感染69例,混合感染45例,其中HBoV混合其他病毒感染22例(45.83％,22/48),HRV-C混合其他病毒感染14例(29.78％,14/47),新型甲型流感H1N1病毒混合其他病毒感染4例(4/13),HMPV混合其他病毒感染9例(9/10),WU多瘤病毒混合其他病毒感染3例(3/3).结论 PICU中HBoV、HMPV、HRV-C、新型甲型流感H1N1病毒等新型呼吸道病毒具有较高的检出率,可能导致重症感染,应该引起临床重视.
目的 瞭解新型呼吸道病毒在PICU中的檢齣情況及感染患兒的臨床特點.方法 收集我院600例PICU急性呼吸道感染患兒的嚥拭子標本600份,87例社區健康體檢兒童嚥拭子87份作為健康對照.應用多重PCR技術對新型呼吸道病毒進行檢測,部分暘性結果進行基因測序,併分析暘性病例的臨床資料.結果 (1) 600例患兒中,新型呼吸道病毒檢測暘性114例(19％),其中人博卡病毒(human bocavirus,HBoV)48例(8.00％),C型鼻病毒(human rhinovirus group C,HRV-C)47例(7.83％),新型甲型流感H1N1病毒13例(2.17％),人偏肺病毒(human metapneumovirs,HMPV)10例(1.67％),WU多瘤病毒3例(0.50％).87例健康兒童呼吸道病毒檢測均為陰性.(2) 114例新型呼吸道病毒檢測暘性患兒中,單一感染69例,混閤感染45例,其中HBoV混閤其他病毒感染22例(45.83％,22/48),HRV-C混閤其他病毒感染14例(29.78％,14/47),新型甲型流感H1N1病毒混閤其他病毒感染4例(4/13),HMPV混閤其他病毒感染9例(9/10),WU多瘤病毒混閤其他病毒感染3例(3/3).結論 PICU中HBoV、HMPV、HRV-C、新型甲型流感H1N1病毒等新型呼吸道病毒具有較高的檢齣率,可能導緻重癥感染,應該引起臨床重視.
목적 료해신형호흡도병독재PICU중적검출정황급감염환인적림상특점.방법 수집아원600례PICU급성호흡도감염환인적인식자표본600빈,87례사구건강체검인동인식자87빈작위건강대조.응용다중PCR기술대신형호흡도병독진행검측,부분양성결과진행기인측서,병분석양성병례적림상자료.결과 (1) 600례환인중,신형호흡도병독검측양성114례(19％),기중인박잡병독(human bocavirus,HBoV)48례(8.00％),C형비병독(human rhinovirus group C,HRV-C)47례(7.83％),신형갑형류감H1N1병독13례(2.17％),인편폐병독(human metapneumovirs,HMPV)10례(1.67％),WU다류병독3례(0.50％).87례건강인동호흡도병독검측균위음성.(2) 114례신형호흡도병독검측양성환인중,단일감염69례,혼합감염45례,기중HBoV혼합기타병독감염22례(45.83％,22/48),HRV-C혼합기타병독감염14례(29.78％,14/47),신형갑형류감H1N1병독혼합기타병독감염4례(4/13),HMPV혼합기타병독감염9례(9/10),WU다류병독혼합기타병독감염3례(3/3).결론 PICU중HBoV、HMPV、HRV-C、신형갑형류감H1N1병독등신형호흡도병독구유교고적검출솔,가능도치중증감염,응해인기림상중시.
Objective To describe the detection and clinical characterization of emerging respiratory virus in PICU.Methods Nasopharyngeal aspirates specimens(NPA) were collected from 600 children who were admitted to PICU with acute respiratory tract infection in our hospital.Additional 87 NPA were collected from healthy children for routine examination in the physical examination center.The clinical datas were collected.Multiplex PCR was applied to detect emerging respiratory virus.Some sequences were identified by using DNA sequencing.And the clinical data of positive cases were analysed.Results (1) There were 114 positive samples(19％) in 600 NPA,including 48(8.00％) human bocavirus(HBoV),47 (7.83％) human rhinovirus group C(HRV-C),13 (2.17％) swine H1N1 (SWH1N1),10 (1.67％) human metapneumovirs (HMPV),3 (0.50％) WU polyomaviruses (WUPyV).But none from 87 healthy controls were positive for any respiratory virus.(2) Co-infection by at least 2 viral pathogens under study was observed in 45 of the 114 positive cases,including 22 (45.83％,22/48) HBoV,14 (29.78％,14/47) HRV-C,4 (4/13) SWH1N1,9(9/10) HMPV,3 (3/3) WU polyomaviruses.Conclusion The emerging viruses,such as HBoV,HMPV,HRV-C,SWH1 N1,have high detection rate in PICU,may lead to severe infection.The clinical doctors should pay attention to them.
