CAJ | 학술논문

目的研究黄芩苷对错配修复基因hMLH1缺失的结直肠癌HCT116细胞裸鼠原位移植瘤细胞周期和凋亡的影响.方法建立人结直肠癌HCT116细胞裸鼠绿色荧光原位移植模型.根据随机数字表法将60只荷瘤裸鼠随机分为对照组和50、100、200 mg/kg黄芩苷组,每组15只.50、100、200 mg/kg黄芩苷组分别给予相应剂量的黄芩苷灌胃,2次/d；对照组采用等容量5％ NaHCO3灌胃.应用流式细胞术检测肿瘤组织HCT116-GFP细胞周期,TUNEL法观察细胞凋亡情况.组间比较采用方差分析或x2检验,两两比较采用LSD-t检验.结果裸鼠模型均构建成功,4组裸鼠体质量和肿瘤体积比较,差异无统计学意义(F =0.343,0.107,P＞0.05).50、100、200 mg/kg黄芩苷组结直肠癌细胞主要阻滞在G2/M期,其构成比分别为22％±6％、18％±7％和19％±6％,与对照组的7％±5％比较,差异有统计学意义(t=5.421,3.483,3.575,P＜0.05)；50、100、200 mg/kg黄芩苷组间比较,差异无统计学意义(F=1.291,P＞0.05).50、100、200 mg/kg黄芩苷组细胞凋亡率分别为59.1％±10.0％、31.5％±9.3％、28.2％±10.8％,与对照组的2.3％±0.9％比较,差异有统计学意义(t=7.163,3.703,2.688,P＜0.05).50 mg/kg黄芩苷组结直肠癌细胞凋亡率显著高于200 mg/kg黄芩苷组(t=2.259,P＜0.05).结论黄芩苷对错配修复基因hMLH1缺失的结直肠癌HCT116细胞裸鼠原位移植瘤生长具有明显的抑制作用.黄芩苷干预后移植瘤细胞周期在G2/M阻滞并诱导细胞凋亡,从而抑制HCT116细胞裸鼠原位移植瘤生长.
목적 연구황금감대착배수복기인hMLH1결실적결직장암HCT116세포라서원위이식류세포주기화조망적영향.방법 건립인결직장암HCT116세포라서록색형광원위이식모형.근거수궤수자표법장60지하류라서수궤분위대조조화50、100、200 mg/kg황금감조,매조15지.50、100、200 mg/kg황금감조분별급여상응제량적황금감관위,2차/d；대조조채용등용량5％ NaHCO3관위.응용류식세포술검측종류조직HCT116-GFP세포주기,TUNEL법관찰세포조망정황.조간비교채용방차분석혹x2검험,량량비교채용LSD-t검험.결과 라서모형균구건성공,4조라서체질량화종류체적비교,차이무통계학의의(F =0.343,0.107,P＞0.05).50、100、200 mg/kg황금감조결직장암세포주요조체재G2/M기,기구성비분별위22％±6％、18％±7％화19％±6％,여대조조적7％±5％비교,차이유통계학의의(t=5.421,3.483,3.575,P＜0.05)；50、100、200 mg/kg황금감조간비교,차이무통계학의의(F=1.291,P＞0.05).50、100、200 mg/kg황금감조세포조망솔분별위59.1％±10.0％、31.5％±9.3％、28.2％±10.8％,여대조조적2.3％±0.9％비교,차이유통계학의의(t=7.163,3.703,2.688,P＜0.05).50 mg/kg황금감조결직장암세포조망솔현저고우200 mg/kg황금감조(t=2.259,P＜0.05).결론 황금감대착배수복기인hMLH1결실적결직장암HCT116세포라서원위이식류생장구유명현적억제작용.황금감간예후이식류세포주기재G2/M조체병유도세포조망,종이억제HCT116세포라서원위이식류생장.
Objective To study the effect of baicalin on the apoptosis and cell cycle of colorectal cancer cells in orthotopic transplantation mice model with mismatch repair gene hMLH1 deficient.Methods Sixty orthotopic transplantation mice models of human colorectal cancer cell line HCT1 16 expressing green fluorescent protein (GFP) were established,and were randomly divided into the control group and the 50,100,200 mg/kg baicalin groups according to the random number table.Mice in the 50,100,200 mg/kg baicalin groups received intragastric infusion of baicalin at the corresponding dosages twice a day,while mice in the control group received intragastric infusion of 5％ NaHCO3.Cell cycles and apoptotic rates of the HCT116-GFP cells were detected by flow cytometry and TUNEL method respectively.Differences between the 2 groups were analyzed using the analysis of variance or chi-square test,and differences within each group were analyzed using the LSD-t test.Results The orthotopic transplantation mice models of human colorecta] cancer were successfully constructed,and there was no significant difference in the body weight of the mice and tumor size among the 4 groups (F =0.343,0.107,P ＞0.05).The proportion of HCT116-GFP cells in the G2/M phase in the 50,100,200 mg/kg baicalin groups were 22％±6％,18％±7％ and 19％±6％,which were significantly higher than 7％ ±5％ of the control group (t =5.421,3.483,3.575,P ＜0.05).There were no significant differences in the proportion of HCT116-GFP cells in the G2/M phase among the 50,100,200 mg/kg baicalin groups (F =1.291,P ＞ 0.05).The apoptotic rates of HCT116-GFP cells in the 50,100,200 mg/kg baicalin groups were significantly higher than the control group (t =7.163,3.703,2.688,P ＜0.05).The apoptotic rate of the 50 mg/kg baicalin group was significantly higher than that of the 200 mg/kg baicalin group (t =2.259,P ＜ 0.05).Conclusions Baicalin significantly inhibits tumor growth in the orthotopic transplantation mice model with mismatch repair gene hMLH1 deficient.After treated with baicalin,the cell cycle is arrested at the G2/M phase,thus the tumor growth is inhibited.