中华小儿外科杂志
中華小兒外科雜誌
중화소인외과잡지
CHINESE JOURNAL OF PEDIATRIC SURGERY
2012年
11期
850-853
,共4页
姜大朋%李昭铸%张玉波%韩福友%管声扬%蒋志涛
薑大朋%李昭鑄%張玉波%韓福友%管聲颺%蔣誌濤
강대붕%리소주%장옥파%한복우%관성양%장지도
硫氧还蛋白%睾丸扭转%缺血再灌注损伤%超氧化物歧化酶%丙二醛
硫氧還蛋白%睪汍扭轉%缺血再灌註損傷%超氧化物歧化酶%丙二醛
류양환단백%고환뉴전%결혈재관주손상%초양화물기화매%병이철
Thioredoxin%Testicular torsion%Ischemia-reperfusion injury%Superoxide dismutase%Malondialdehyde
目的 探讨硫氧还蛋白对大鼠睾丸缺血再灌注损伤的保护作用.方法 选取雄性SD大鼠60只,随机分为4组:假手术对照组(A组);睾丸扭转/复位组(B组);睾丸扭转/复位+腹腔内注射生理盐水组(C组);睾丸扭转/复位+腹腔内注射硫氧还蛋白组(D组).无菌条件下制作左侧睾丸扭转模型,扭转持续4h,复位4h后取睾丸标本(D组在复位前15 min腹腔内注射硫氧还蛋白).对标本进行病理组织学检查;检测睾丸组织中超氧化物歧化酶(SOD)和丙二醛(MDA)的含量.结果 睾丸扭转/复位后可见生精小管退变,间质出现水肿及出血,腹腔注射硫氧还蛋白使睾丸扭转/复位诱发的组织学改变明显改善.B组及C组睾丸损伤评分(8.3±0.96;8±0.87)明显高于A组(0.78±0.36)(P＜0.01),而腹腔注射硫氧还蛋白可以使睾丸损伤分值(3.1±0.42)显著降低(P＜0.05).B组及C组MDA含量(4.39±0.21;4.42±0.17)升高,SOD活性(269±27.1;271±21.3)降低,与对照组(MDA:1.61±0.18;SOD:317±22.3)相比,差别具有显著性意义(P＜0.01).而腹腔注射硫氧还蛋白能有效降低MDA含量(2.03±0.03)并升高SOD活性(315±24.2)(P＜0.01).结论 本实验为硫氧还蛋白作为治疗睾丸扭转继发损害的有效物质提供了组织学及生化依据.为临床预防和治疗睾丸缺血再灌注损伤提供了理论依据.
目的 探討硫氧還蛋白對大鼠睪汍缺血再灌註損傷的保護作用.方法 選取雄性SD大鼠60隻,隨機分為4組:假手術對照組(A組);睪汍扭轉/複位組(B組);睪汍扭轉/複位+腹腔內註射生理鹽水組(C組);睪汍扭轉/複位+腹腔內註射硫氧還蛋白組(D組).無菌條件下製作左側睪汍扭轉模型,扭轉持續4h,複位4h後取睪汍標本(D組在複位前15 min腹腔內註射硫氧還蛋白).對標本進行病理組織學檢查;檢測睪汍組織中超氧化物歧化酶(SOD)和丙二醛(MDA)的含量.結果 睪汍扭轉/複位後可見生精小管退變,間質齣現水腫及齣血,腹腔註射硫氧還蛋白使睪汍扭轉/複位誘髮的組織學改變明顯改善.B組及C組睪汍損傷評分(8.3±0.96;8±0.87)明顯高于A組(0.78±0.36)(P＜0.01),而腹腔註射硫氧還蛋白可以使睪汍損傷分值(3.1±0.42)顯著降低(P＜0.05).B組及C組MDA含量(4.39±0.21;4.42±0.17)升高,SOD活性(269±27.1;271±21.3)降低,與對照組(MDA:1.61±0.18;SOD:317±22.3)相比,差彆具有顯著性意義(P＜0.01).而腹腔註射硫氧還蛋白能有效降低MDA含量(2.03±0.03)併升高SOD活性(315±24.2)(P＜0.01).結論 本實驗為硫氧還蛋白作為治療睪汍扭轉繼髮損害的有效物質提供瞭組織學及生化依據.為臨床預防和治療睪汍缺血再灌註損傷提供瞭理論依據.
목적 탐토류양환단백대대서고환결혈재관주손상적보호작용.방법 선취웅성SD대서60지,수궤분위4조:가수술대조조(A조);고환뉴전/복위조(B조);고환뉴전/복위+복강내주사생리염수조(C조);고환뉴전/복위+복강내주사류양환단백조(D조).무균조건하제작좌측고환뉴전모형,뉴전지속4h,복위4h후취고환표본(D조재복위전15 min복강내주사류양환단백).대표본진행병리조직학검사;검측고환조직중초양화물기화매(SOD)화병이철(MDA)적함량.결과 고환뉴전/복위후가견생정소관퇴변,간질출현수종급출혈,복강주사류양환단백사고환뉴전/복위유발적조직학개변명현개선.B조급C조고환손상평분(8.3±0.96;8±0.87)명현고우A조(0.78±0.36)(P＜0.01),이복강주사류양환단백가이사고환손상분치(3.1±0.42)현저강저(P＜0.05).B조급C조MDA함량(4.39±0.21;4.42±0.17)승고,SOD활성(269±27.1;271±21.3)강저,여대조조(MDA:1.61±0.18;SOD:317±22.3)상비,차별구유현저성의의(P＜0.01).이복강주사류양환단백능유효강저MDA함량(2.03±0.03)병승고SOD활성(315±24.2)(P＜0.01).결론 본실험위류양환단백작위치료고환뉴전계발손해적유효물질제공료조직학급생화의거.위림상예방화치료고환결혈재관주손상제공료이론의거.
Objective The aim of this study was to examine the effectiveness of thioredoxin on the prevention of testicular damage induced by ischemia/reperfusion(I/R)in rats.Methods Male Sprague Dawley rats(n=60)were divided randomly into 4 experimental groups:sham group,torsiondetorsion(TD)group,TD+saline group,and TD+thioredoxin group.Testicular ischemia was achieved by twisting the left testis for 4 hours,and reperfusion was allowed for 4 hours after detorsion.Human thioredoxin was injected intraperitoneally to rats in D group at time point of 15 min before detorsion.Left orchiectomies were harvested for tissue histopathological examinations.The detection of superoxide dismutase(SOD)activities and malondialdehyde(MDA)levels were also conducted.Results Interstitial edema,dilatation,and degeneration of germ cells in tubules were observed after TD.Testicular tissues in D group showed an improved histological morphology.Testicular injury score in B group and C group(8.3±0.96;8±0.87)were significantly increased compared with those in the Sham group(0.78±0.36)(P＜0.01).It was observed that scores(3.1±0.42)were significantly lower in D group compared with B and C groups(P＜0.05).The MDA concentration(4.39±0.21;4.42±0.17)and SOD activities(269±27.1;271±21.3)in B and C group were increased and decreased respectively as compared with the Sham group(MDA:1.61±0.18;SOD:317±22.3)(P＜0.01).Treatment of rats with human thioredoxin significantly reduced the tissue MDA levels(2.03±0.03)(P＜0.01).SOD activities(315±24.2)were increased in testes in D group(P＜0.01).Conclusions The results provide biochemical and histopathological evidence for human thioredoxin to be used as a therapeutic agent for the testicular damage induced by I/R injury.
