CAJ | 학술논문

目的探讨PI3K/Akt信号通路在小儿血管瘤的发生、发展及消退过程中的作用.方法收集未经其他治疗、单纯手术切除并病理诊断为血管瘤的标本,结合Mulliken分类法与增殖细胞核抗原(PCNA)表达对血管瘤进行分类和分期,抽取增生期与消退期血管瘤各20例,免疫组化检测增生期血管瘤和消退期血管瘤组织中磷脂酰肌醇3激酶(PI3K,p85)和磷酸化蛋白激酶B(p-Akt)的表达水平,其中液氮冻存的增生期和消退期血管瘤标本各11例,应用RT-PCR检测p85、p-Akt的mRNA表达情况,并分析其相关性.结果增生期血管瘤p85、p-Akt的表达率和表达强度均高于消退期血管瘤(P＜0.05,P＜0.01)；增生期血管瘤p85、p-Akt的积分光密度分别为191.23±40.45、178.73±38.49,消退期血管瘤p85、pAkt的积分光密度分别为46.63±13.08、64.93±21.22,差异有统计学意义(P＜0.01);p85与p-Akt的表达之间呈正相关关系(r=0.553 0,P＜0.05).增生期血管瘤p85 mRNA、p-Akt mRNA的相对表达值分别为0.0807±0.0136、0.0841±0.0137,消退期血管瘤p85、P-Akt的相对表达值分别为0.0203±0.0098、0.0324±0.0178,差异有统计学意义(P＜0.01);p85mRNA、p-Akt mRNA的表达之间呈正相关关系(r=0.5503,P＜0.01).结论小儿增生期和消退期血管瘤组织中p85、p-Akt及其mRNA表达有差异,PI3K/Akt信号通路可能在小儿血管瘤的病理演变中发挥作用.
목적 탐토PI3K/Akt신호통로재소인혈관류적발생、발전급소퇴과정중적작용.방법 수집미경기타치료、단순수술절제병병리진단위혈관류적표본,결합Mulliken분류법여증식세포핵항원(PCNA)표체대혈관류진행분류화분기,추취증생기여소퇴기혈관류각20례,면역조화검측증생기혈관류화소퇴기혈관류조직중린지선기순3격매(PI3K,p85)화린산화단백격매B(p-Akt)적표체수평,기중액담동존적증생기화소퇴기혈관류표본각11례,응용RT-PCR검측p85、p-Akt적mRNA표체정황,병분석기상관성.결과 증생기혈관류p85、p-Akt적표체솔화표체강도균고우소퇴기혈관류(P＜0.05,P＜0.01)；증생기혈관류p85、p-Akt적적분광밀도분별위191.23±40.45、178.73±38.49,소퇴기혈관류p85、pAkt적적분광밀도분별위46.63±13.08、64.93±21.22,차이유통계학의의(P＜0.01);p85여p-Akt적표체지간정정상관관계(r=0.553 0,P＜0.05).증생기혈관류p85 mRNA、p-Akt mRNA적상대표체치분별위0.0807±0.0136、0.0841±0.0137,소퇴기혈관류p85、P-Akt적상대표체치분별위0.0203±0.0098、0.0324±0.0178,차이유통계학의의(P＜0.01);p85mRNA、p-Akt mRNA적표체지간정정상관관계(r=0.5503,P＜0.01).결론 소인증생기화소퇴기혈관류조직중p85、p-Akt급기mRNA표체유차이,PI3K/Akt신호통로가능재소인혈관류적병리연변중발휘작용.
Objective To study the role of PI3K/Akt signal pathways in the different phases of hemangioma in children.Methods Specimens of hemangioma were collected during surgery.Proliferating and involuting phases of hemangioma were determined by Mulliken's classification and expression of proliferating cell nuclear antigen (PCNA).Twenty proliferating hemangioma and 20 involuting hemangioma were recruited in this study.The expressions of p85 (n =20) and p-Akt (n =20) were studied by immunohistochemical staining.The p85-mRNA (n =11) and p-Akt-mRNA (n =11) were detected by reverse transcription polymeras chain reaction.Results In proliferating hemangioma,the percentage of p85 and p-Akt positive cells was higher than that of involuting hemangioma (all P＜0.05).In proliferating hemangioma,the integral optical density (IOD) of p85 and p-Akt of the immunoreactive cells was 191.23 ± 40.45,78.73 ± 38.49,respectively.In involuting hemangioma,the IOD of p85 and p-Akt of the immunoreactive cells was 46.63 ± 13.08,64.93 ± 21.22,respectively.The IOD of p85 and p-Akt of the immunoreactive cells in proliferating hemangioma were statistically higher than those in involuting hemangioma (P＜0.01).Moreover,the expression of p85 was positively correlated with the expression of p-Akt (r =0.5530,P＜0.05).The mRNA relative expression levels of p85 and p-Akt in proliferatin hemangioma were 0.0807 ± 0.0136,0.0841 ± 0.0137,respectively.In involuting hemangioma,the mRNA relative expression levels of p85 and p-Akt were 0.0203 ± 0.0098,0.0324± 0.0178,respectively.The mRNA relative expression levels of p85 and p-Akt in proliferating hemangioma were statistically higher than those in involuting hemangioma (P＜0.01).Moreover,the mRNA relative expression level of p85 was positively correlated with that of p-Akt (r =0.5503,P＜0.01).Conclusions The expressions of p85 and p-Akt are increased in hemangioma,which suggests PI3K/Akt signaling pathway may play a role in the pathogenesis of hemangioma.