CAJ | 학술논문

目的研究Smads在正常和尿道下裂患儿包皮组织中的表达,探讨其在尿道下裂发病机制中的角色.方法收集拟行尿道下裂尿道修复术患儿术中切除的包皮组织(30例)和门诊包皮环切术中切除的儿童包皮(30例),采用免疫组织化学和实时定量聚合酶链反应(qPCR)方法检测两组包皮组织中Smad 7、Smad 3和Smad 2的mRNA和蛋白质的表达变化.结果尿道下裂患儿包皮组织中Smad 7、Smad 3和Smad2 mRNA表达较正常水平分别升高97.0％,22.8％和59.9％；且均有显著性差异(P＜0.05).Smad 7和磷酸化Smad 2/3蛋白表达于包皮皮下间充质细胞层,且在尿道下裂患儿包皮组织的表达较正常对照有显著升高趋势.结论根据结果我们初步认为,Smad作为细胞间信号传递蛋白参与了男性尿道的发育,而Smad 7、Smad3和Smad 2过表达可能是调控尿道下裂发生的重要因素.
목적 연구Smads재정상화뇨도하렬환인포피조직중적표체,탐토기재뇨도하렬발병궤제중적각색.방법 수집의행뇨도하렬뇨도수복술환인술중절제적포피조직(30례)화문진포피배절술중절제적인동포피(30례),채용면역조직화학화실시정량취합매련반응(qPCR)방법검측량조포피조직중Smad 7、Smad 3화Smad 2적mRNA화단백질적표체변화.결과 뇨도하렬환인포피조직중Smad 7、Smad 3화Smad2 mRNA표체교정상수평분별승고97.0％,22.8％화59.9％；차균유현저성차이(P＜0.05).Smad 7화린산화Smad 2/3단백표체우포피피하간충질세포층,차재뇨도하렬환인포피조직적표체교정상대조유현저승고추세.결론 근거결과아문초보인위,Smad작위세포간신호전체단백삼여료남성뇨도적발육,이Smad 7、Smad3화Smad 2과표체가능시조공뇨도하렬발생적중요인소.
Objective To study the expressions of Smad7,Smad3 and Smad2 in foreskin from children with hypospadias.Methods Penile foreskin tissues were collected from 30 children during repair surgery for hypospadias.The control foreskin tissues were collected from age-paired 30 children underwent circumcision.Real-time quantitative polymerase chain reaction (qPCR) and immunochemical staining were employed to determine the mRNA expression and phosphorylated Smad7,Smad3 and Smad2 in foreskin tissues.Results The mRNAs of Smad7,Smad3 and Smad2 in foreskin tissues respectively increased by 97.0％,22.8％ and 59.9％ in hypospadias patients compared with those in control patients,and the difference between the 2 groups was significant (all P＜0.05).Meanwhile,the phosphorylated Smad7,Smad3 and Smad2 were mainly located in mesenchymal cells in foreskin,and their expressions were significantly increased in hypospadias compared with those in controls (all P＜0.05).Conclusions The increased expressions of Smad7,Smad3 and Smad2 are associated with hypospadias,which may play a role in the development of urethra and formation of hypospadias.