CAJ | 학술논문

目的探讨利多卡因对肠缺血-再灌注(ischemia/reperfusion,I/R)损伤幼鼠肠组织表达肿瘤坏死因子α(TNF-α)和细胞间粘附分子1(ICAM-1)的影响.方法将48只4周龄SD大鼠随机分成三组,每组16只:假手术组(Sham组),仅暴露腹腔脏器,不作任何干预；缺血-再灌注组(I/R组)和利多卡因干预组(Lid组),阻断肠系膜上动脉造成缺血60 min,I/R组再灌注前经股静脉注射生理盐水0.5ml,Lid组再灌注前经股静脉注射利多卡因2 mg/kg.于再灌注后30、60、90、120min每组动物各处死4只取小肠组织进行免疫组织化学染色,检测肠组织TNF-α、ICAM-1的表达,结果进行组间单因素方差分析.结果正常肠组织中TNF-α和ICAM-1呈低水平表达,而从再灌注30 min开始在I/R组和Lid组肠组织中TNF-α呈持续的高表达状态,但这两组中ICAM-1的表达呈缓慢上升趋势；通过测定TNF-α、ICAM-1表达的光密度值(OD值)在组间比较,I/R组和Lid组各时间点测得值均高于Sham组的相应测得值,差异有统计学意义(P＜0.05),其中两个指标均以I/R组的测得值为最高,差异有显著性(P＜0.05),仅ICAM-1于30、60min表达的OD值在I/R组和Lid组间比较差异无统计学意义(P＞0.05).结论幼鼠再灌注肠组织中TNF-α和ICAM-1的表达增加,利多卡因通过下调肠组织中TNF-α、ICAM-1的表达而对生长发育期动物肠组织I/R损伤有保护作用.
목적 탐토리다잡인대장결혈-재관주(ischemia/reperfusion,I/R)손상유서장조직표체종류배사인자α(TNF-α)화세포간점부분자1(ICAM-1)적영향.방법 장48지4주령SD대서수궤분성삼조,매조16지:가수술조(Sham조),부폭로복강장기,불작임하간예；결혈-재관주조(I/R조)화리다잡인간예조(Lid조),조단장계막상동맥조성결혈60 min,I/R조재관주전경고정맥주사생리염수0.5ml,Lid조재관주전경고정맥주사리다잡인2 mg/kg.우재관주후30、60、90、120min매조동물각처사4지취소장조직진행면역조직화학염색,검측장조직TNF-α、ICAM-1적표체,결과진행조간단인소방차분석.결과 정상장조직중TNF-α화ICAM-1정저수평표체,이종재관주30 min개시재I/R조화Lid조장조직중TNF-α정지속적고표체상태,단저량조중ICAM-1적표체정완만상승추세；통과측정TNF-α、ICAM-1표체적광밀도치(OD치)재조간비교,I/R조화Lid조각시간점측득치균고우Sham조적상응측득치,차이유통계학의의(P＜0.05),기중량개지표균이I/R조적측득치위최고,차이유현저성(P＜0.05),부ICAM-1우30、60min표체적OD치재I/R조화Lid조간비교차이무통계학의의(P＞0.05).결론 유서재관주장조직중TNF-α화ICAM-1적표체증가,리다잡인통과하조장조직중TNF-α、ICAM-1적표체이대생장발육기동물장조직I/R손상유보호작용.
Objective To explore the effects of lidocaine on the expressions of tumor necrosis factor-a (TNF-α) and intercellular adhesion molecule-1 (ICAM-1) in intestines subjected to ischemia/reperfusion (I/R) injury.Methods In this study,48 Sprague-Dawley (SD) rats aged 4 weeks old were randomly divided into 3 groups according to their treatment.In the I/R group,the abdominal cavity of the rats was opened,and the intestinal I/R injury was induced by 60 minutes' superior mesenteric artery occlusion followed by reperfusion for 30 min,60 min,90 min and 120 min.In the lidocaine treatment group,the rats were subjected to intestinal I/R injury,and administered with Lidocaine by femoral vein injection before reperfusion.The sham operated rats were only performed laparotomy.The intestines were harvested for immunohistochemical study to explore the expressions of TNF-α and ICAM-1.Results The sham operated rats had low TNF-α and ICAM-1 expressions.After I/R injury,the expressions of TNF-α and ICAM-1 in intestine were significantly elevated,but Lidocaine treatment inhibited the I/R induced up-regulation of TNF-α and ICAM-1 (all P＜0.05).The intestinal expression of ICAM-1 remained unchanged in 30 min and 60 rnin after I/R (all P＞0.05).Conclusions Lidocaine down-regulates the expressions of TNF-α and ICAM-1 in intestine after ischemia/reperfusion injury in young rats.