CAJ | 학술논문

目的建立幼鼠高位脊髓损伤神经源性膀胱(neurogenic bladder,NB)模型并对其进行尿动力学评估,为临床研究神经源性膀胱的病理生理提供一定参考.方法 22只健康Wistar雌性大白鼠,体重(200±20)g,4～6周龄,随机分为NB组(n=14)和正常对照组(假手术组,n=8).NB组采用改良的Allen,s法(冲击损伤法50 gcm)建T12脊髓损伤模型.观察对照组与NB组术后5～6 d神经损害的表现并进行影像尿动力学评估.结果 NB组大白鼠术后出现后肢瘫痪等脊髓损伤表现,对照组(n=8)无神经损伤表现.和对照组相比影像尿动力示NB组逼尿肌无收缩,膀胱最大容量[maximum bladder capacity,MBC,(3.93±2.03)ml比(0.081±0.247) ml,P＜0.001]和膀胱顺应性(bladder compliance,BC,0.093±0.54比0.022±0.01,P＜0.001)显著增加,逼尿肌漏尿点压(leak detrusor pressur,LDP,45.38±12.52比26±3.85,P＜0.001)显著升高.结论采用改良的Allen's法(冲击损伤法50gcm)能建立幼鼠高位脊髓损伤NB模型,尿动力学检查符合NB改变.
목적 건립유서고위척수손상신경원성방광(neurogenic bladder,NB)모형병대기진행뇨동역학평고,위림상연구신경원성방광적병리생리제공일정삼고.방법 22지건강Wistar자성대백서,체중(200±20)g,4～6주령,수궤분위NB조(n=14)화정상대조조(가수술조,n=8).NB조채용개량적Allen,s법(충격손상법50 gcm)건T12척수손상모형.관찰대조조여NB조술후5～6 d신경손해적표현병진행영상뇨동역학평고.결과 NB조대백서술후출현후지탄탄등척수손상표현,대조조(n=8)무신경손상표현.화대조조상비영상뇨동력시NB조핍뇨기무수축,방광최대용량[maximum bladder capacity,MBC,(3.93±2.03)ml비(0.081±0.247) ml,P＜0.001]화방광순응성(bladder compliance,BC,0.093±0.54비0.022±0.01,P＜0.001)현저증가,핍뇨기루뇨점압(leak detrusor pressur,LDP,45.38±12.52비26±3.85,P＜0.001)현저승고.결론 채용개량적Allen's법(충격손상법50gcm)능건립유서고위척수손상NB모형,뇨동역학검사부합NB개변.
Objective To evaluate urodynamic study in young rats with neurogenic bladder (NB) induced by high-level spinal cord injury.Methods Twenty-two young female Wistar rats [mean weight (200 g ± 20),4-6 weeks old] were randomly divided into NB group (n =14) and normal control group (n =8).NB group was made using modified method of Allen's (Impact damage method 50 gcm)at the twelve thoracic spine level.Video-urodynamic assessment was performed at 5-6 days post injury.Results NB group showed typical postoperative manifestations of hind limb paralysis.The control group showed no significant abnormality.Detrusor muscle showed areflexia,maximum bladder capacity (MBC) and leak detrusor pressure (LDP) and bladder compliance (BC) increased significantly in NB group than those of control group [MBC(3.93 ± 2.03)ml vs.(0.081 ± 0.247)ml; BC(0.093 ±0.54) ml vs.(0.022 ±0.01)ml; LDP(45.38 ± 12.52)ml vs.(26 ± 3.85)ml respectively] (P＜0.001).Conclusions The modified method of Allen's is useful to establish NB.