中华小儿外科杂志
中華小兒外科雜誌
중화소인외과잡지
CHINESE JOURNAL OF PEDIATRIC SURGERY
2013年
8期
622-625
,共4页
李泸平%范应中%孙生安%李正伟%王家祥%张谦%张胜利%郭立华%冯春祥
李瀘平%範應中%孫生安%李正偉%王傢祥%張謙%張勝利%郭立華%馮春祥
리로평%범응중%손생안%리정위%왕가상%장겸%장성리%곽립화%풍춘상
干细胞%细胞外基质%尿道重建
榦細胞%細胞外基質%尿道重建
간세포%세포외기질%뇨도중건
Stem cells%Extracellular matrix%Urethral reconstruction
目的 探讨应用负载同种脂肪干细胞的异种真皮脱细胞基质生物复合材料修复兔尿道缺损的效果.方法 将54只新西兰雄性大白兔随机均分三组,A组(实验组)应用负载脂肪干细胞的异种真皮脱细胞基质修复缺损兔尿道,B组(对照组Ⅰ)用真皮脱细胞基质材料进行修复,C组(对照组Ⅱ)将尿道断端直接缝合.于术后2、4、7、16周切取尿道标本,行组织学观察并比较各组间毛细血管及平滑肌数有无差异.术前术后行尿动力检查,比较最大尿道压有无差别.结果 术后组织学观察A组可形成近似正常的尿道结构,B和C组尿道结构不如A组.术后7周时比较A、B、C三组新生尿道毛细血管数(15.8±2.5比12.3±2.6比7.1±2.3)及平滑肌数(18.1±3.1比13.5±2.7比8.9±2.6),A组多于B、C组,且组间差异有统计学意义(P<0.05).A组术前术后最大尿道压无明显差别[(24.3±2.6)cmH2O比(25.2 ±2.0)cmH2O,P>0.05],B组术前术后差别有统计学意义[(23.6±2.3)cmH2O比(28.7±2.0)cmH2O,P<0.01].结论 脂肪干细胞作为种子细胞可用于构建组织工程化尿道;脂肪干细胞复合脱细胞基质能加速新生尿道毛细血管及平滑肌再生,有助于减少术后并发症.
目的 探討應用負載同種脂肪榦細胞的異種真皮脫細胞基質生物複閤材料脩複兔尿道缺損的效果.方法 將54隻新西蘭雄性大白兔隨機均分三組,A組(實驗組)應用負載脂肪榦細胞的異種真皮脫細胞基質脩複缺損兔尿道,B組(對照組Ⅰ)用真皮脫細胞基質材料進行脩複,C組(對照組Ⅱ)將尿道斷耑直接縫閤.于術後2、4、7、16週切取尿道標本,行組織學觀察併比較各組間毛細血管及平滑肌數有無差異.術前術後行尿動力檢查,比較最大尿道壓有無差彆.結果 術後組織學觀察A組可形成近似正常的尿道結構,B和C組尿道結構不如A組.術後7週時比較A、B、C三組新生尿道毛細血管數(15.8±2.5比12.3±2.6比7.1±2.3)及平滑肌數(18.1±3.1比13.5±2.7比8.9±2.6),A組多于B、C組,且組間差異有統計學意義(P<0.05).A組術前術後最大尿道壓無明顯差彆[(24.3±2.6)cmH2O比(25.2 ±2.0)cmH2O,P>0.05],B組術前術後差彆有統計學意義[(23.6±2.3)cmH2O比(28.7±2.0)cmH2O,P<0.01].結論 脂肪榦細胞作為種子細胞可用于構建組織工程化尿道;脂肪榦細胞複閤脫細胞基質能加速新生尿道毛細血管及平滑肌再生,有助于減少術後併髮癥.
목적 탐토응용부재동충지방간세포적이충진피탈세포기질생물복합재료수복토뇨도결손적효과.방법 장54지신서란웅성대백토수궤균분삼조,A조(실험조)응용부재지방간세포적이충진피탈세포기질수복결손토뇨도,B조(대조조Ⅰ)용진피탈세포기질재료진행수복,C조(대조조Ⅱ)장뇨도단단직접봉합.우술후2、4、7、16주절취뇨도표본,행조직학관찰병비교각조간모세혈관급평활기수유무차이.술전술후행뇨동력검사,비교최대뇨도압유무차별.결과 술후조직학관찰A조가형성근사정상적뇨도결구,B화C조뇨도결구불여A조.술후7주시비교A、B、C삼조신생뇨도모세혈관수(15.8±2.5비12.3±2.6비7.1±2.3)급평활기수(18.1±3.1비13.5±2.7비8.9±2.6),A조다우B、C조,차조간차이유통계학의의(P<0.05).A조술전술후최대뇨도압무명현차별[(24.3±2.6)cmH2O비(25.2 ±2.0)cmH2O,P>0.05],B조술전술후차별유통계학의의[(23.6±2.3)cmH2O비(28.7±2.0)cmH2O,P<0.01].결론 지방간세포작위충자세포가용우구건조직공정화뇨도;지방간세포복합탈세포기질능가속신생뇨도모세혈관급평활기재생,유조우감소술후병발증.
Objective To evaluate the effect of urethral repair by heterologous dermal acellular matrix seeded with rabbit adipose derived stem cells (ADSCs).Methods Fifty-four rabbits were randomly divided into three groups.Group A (experimental group):heterologous dermal acellular matrix with ADSCs were used for repairing a 2 cm length of urethral defect.Group B (control group Ⅰ):repaired only with heterologous dermal acellular matrix.Group C (control group Ⅱ):urethral defect was sutured and repaired simply.The newly laid down urethral tissue was harvested regularly (2,4,7 and 16 weeks after surgery) for histological examination.The differences in blood vessels and smooth muscles were compared in the three groups.Urodynamics was tested to compare the differences of maximum urethra pressure before and after surgery.Results Almost normal urethral tissue could be found in group A,and it was better than group B and group C.The number of blood vessels and smooth muscle was much more in group A than the other groups.There was significant difference among them (18.1 ± 3.1 vs 13.5 ± 2.7 vs 8.9 ± 2.6,P<0.05).The maximum urethra pressure in group A showed no significant difference after operation[(24.3 ± 2.6) cmH2O vs (25.2 ± 2.0)cmH2O,P>0.05],but there was significant difference in group B [(23.6 ± 2.3)cmH2O vs (28.7 ± 2.0)cmH2O,P<0.01].Conclusions ADSCs are feasible for the reconstruction of tissue-engineered urethra.Dermal acellular matrix compounded with ADSCs helps to reduce postoperative complications due to accelerated regeneration of new urethral blood vessels and smooth muscles.