中华小儿外科杂志
中華小兒外科雜誌
중화소인외과잡지
CHINESE JOURNAL OF PEDIATRIC SURGERY
2014年
1期
47-50
,共4页
赵占波%王秀良%丁雄辉%孙艳辉%段文娟%金鑫%金先庆
趙佔波%王秀良%丁雄輝%孫豔輝%段文娟%金鑫%金先慶
조점파%왕수량%정웅휘%손염휘%단문연%금흠%금선경
先天性无神经节症%神经节细胞%整联蛋白
先天性無神經節癥%神經節細胞%整聯蛋白
선천성무신경절증%신경절세포%정련단백
Congenital aganglionosis%Ganglion cell%Integrin
目的 探讨β1整联蛋白(integrin)在先天性肠无神经节细胞症(Hirsehsprung disease,HD)患儿不同肠段各层中有无变化及在HD发病中的可能作用.方法 取40例先天性肠无神经节细胞症患儿的痉挛段、移行段、正常段.采用免疫组织化学法染色、Western blot和RT-PCR检测肠壁中β1 integrin蛋白和mRNA的表达情况,并结合图像分析,比较表达的差异.结果 免疫组织化学结果示β1 integrin免疫沉淀产物主要表达在神经节细胞的胞质和胞膜中表达,正常对照组、移行段和痉挛段肠壁中阳性区域平均光密度值分别为0.86±0.16、0.61±0.15和0.35±0.07,呈依次递减,差异具有统计学意义(P＜0.05).β1 integrin在HD狭窄段和移行段肠管中mRNA转录水平的表达分别为0.77±0.17和1.08±0.15,均低于正常对照组的2.26±0.29(P＜0.05).同时Western blot检测显示β1 integrin蛋白在各组中的表达与mRNA水平呈平行结果.结论 β1 integrin在HD痉挛段和移行段的表达减低,β1 integrin可能在HD的发病过程中起到重要的作用.
目的 探討β1整聯蛋白(integrin)在先天性腸無神經節細胞癥(Hirsehsprung disease,HD)患兒不同腸段各層中有無變化及在HD髮病中的可能作用.方法 取40例先天性腸無神經節細胞癥患兒的痙攣段、移行段、正常段.採用免疫組織化學法染色、Western blot和RT-PCR檢測腸壁中β1 integrin蛋白和mRNA的錶達情況,併結閤圖像分析,比較錶達的差異.結果 免疫組織化學結果示β1 integrin免疫沉澱產物主要錶達在神經節細胞的胞質和胞膜中錶達,正常對照組、移行段和痙攣段腸壁中暘性區域平均光密度值分彆為0.86±0.16、0.61±0.15和0.35±0.07,呈依次遞減,差異具有統計學意義(P＜0.05).β1 integrin在HD狹窄段和移行段腸管中mRNA轉錄水平的錶達分彆為0.77±0.17和1.08±0.15,均低于正常對照組的2.26±0.29(P＜0.05).同時Western blot檢測顯示β1 integrin蛋白在各組中的錶達與mRNA水平呈平行結果.結論 β1 integrin在HD痙攣段和移行段的錶達減低,β1 integrin可能在HD的髮病過程中起到重要的作用.
목적 탐토β1정련단백(integrin)재선천성장무신경절세포증(Hirsehsprung disease,HD)환인불동장단각층중유무변화급재HD발병중적가능작용.방법 취40례선천성장무신경절세포증환인적경련단、이행단、정상단.채용면역조직화학법염색、Western blot화RT-PCR검측장벽중β1 integrin단백화mRNA적표체정황,병결합도상분석,비교표체적차이.결과 면역조직화학결과시β1 integrin면역침정산물주요표체재신경절세포적포질화포막중표체,정상대조조、이행단화경련단장벽중양성구역평균광밀도치분별위0.86±0.16、0.61±0.15화0.35±0.07,정의차체감,차이구유통계학의의(P＜0.05).β1 integrin재HD협착단화이행단장관중mRNA전록수평적표체분별위0.77±0.17화1.08±0.15,균저우정상대조조적2.26±0.29(P＜0.05).동시Western blot검측현시β1 integrin단백재각조중적표체여mRNA수평정평행결과.결론 β1 integrin재HD경련단화이행단적표체감저,β1 integrin가능재HD적발병과정중기도중요적작용.
Objective To investigate the expression of β1 integrin (ITGB1) in the tissue samples from patients with Hirschsprung's disease (HD).Methods The stenotic,transitional and normal appearance intestines from forty patients with HD were collected during operation.For comparative gene expression studies,the expression levels of β1 integrin was detected using immunohistochemistry,Real-time PCR (RT-PCR) and western blotting.Results Immunohistochemistry studies shows that β1 integrin immunoprecipitation product was mainly localized to the cytoplasm and membrane of the ganglion cells.The mean optical density(OD) value was 0.86 ± 0.16,0.61 ± 0.15,and 0.35 ± 0.07 (give in descending order) in normal,transitional and stenotic segments of HD,respectively,the difference was statistically significant (P ＜ 0.05).The mRNA expression of β1 integrin in stenotic and transitional segments was significantly lower than that in the normal bowel segment (the relative quantification was 0.77 ± 0.17,1.08 ± 0.15,2.26 ± 0.29,respectively,P＜0.05).The protein expression profile of β1 integrin in these patients were similar to that of mRNA.Conclusions The expression of β1 integrin is decreased in the affected colon from the patients with HD,which may play a role in the pathogenesis of HD.
