中华小儿外科杂志
中華小兒外科雜誌
중화소인외과잡지
CHINESE JOURNAL OF PEDIATRIC SURGERY
2014年
2期
139-143
,共5页
李恩惠%蒋君涛%孙文兰%刘世博%夏术阶%刘志宏%孙晓文
李恩惠%蔣君濤%孫文蘭%劉世博%夏術階%劉誌宏%孫曉文
리은혜%장군도%손문란%류세박%하술계%류지굉%손효문
邻苯甲二酸二丁酯%肛门,畸形%直肠,畸形%肛门闭锁%大鼠
鄰苯甲二痠二丁酯%肛門,畸形%直腸,畸形%肛門閉鎖%大鼠
린분갑이산이정지%항문,기형%직장,기형%항문폐쇄%대서
Dibutyl phthalate%Anus,abnormalities%Rectum,abnormalities%Anus,imperforate%Rats
目的 研究邻苯二甲酸二丁酯(DBP)在大鼠孕晚期染毒诱导子代雄性大鼠发生肛门直肠畸形(ARMs),并探讨DBP对子代雄性大鼠生长发育的影响.方法 孕鼠20只,随机分为两组,怀孕第12～18天,每天分别予大豆油及DBP 850 mg/kg灌胃.出生后(PND) 1d统计肛门闭锁发生率,测量雄性仔鼠体重和肛门生殖器距离(AGD).PND 7 d观察肛门闭锁雄性仔鼠末端直肠及肛周组织的病理学改变.PND 35 d统计雄性仔鼠体重、体长(除尾)和腹围,解剖后对实质脏器(脑、心、肝、脾、肺、肾)以及生殖器官(睾丸、附睾)称重,计算脏器系数,评估发育情况.结果 雄性仔鼠肛门闭锁发生率为39.5％.PND 1d肛门闭锁雄性仔鼠体重及AGD分别为(4.876±0.067)g、(2.401±0.341)mm,对照组分别为(6.365±0.085)g、(2.993±0.403)mm,与对照组相比明显降低,差异有统计学意义(P＜0.05).PND 7 d末端直肠及肛周组织病理显示典型的ARMs.PND 35 d肛门闭锁雄性仔鼠腹围及体长分别为(20.021±0.416) mm、(12.925±0.158)mm,对照组分别为(11.993±0.339)g、(14.943±0.171)mm,与对照组相比,腹围增加且体长缩短,差异有统计学意义(P＜0.05).解剖后发现,肛门闭锁雄性仔鼠均发生巨结肠改变,实质脏器(脑、心、肝、脾、肺、左肾、右肾)以及生殖器官(左睾丸、右睾丸、左附睾、右附睾)脏器系数分别为(9.312±0.233)mg/g、(3.541±0.079) mg/g、(46.382±1.309)mg/g、(3.653±0.098) mg/g、(8.872±0.323)mg/g、(5.261±0.108) mg/g、(5.093±0.096)mg/g、(1.725±0.049) mg/g、(1.513±0.041) mg/g、(0.169±0.009) mg/g、(0.182±0.008) mg/g,对照组分别为(12.452±0.213)mg/g、(4.582±0.128)mg/g、(52.853±1.256) mg/g、(4.012±0.145)mg/g、(10.079±0.292)mg/g、(5.843±0.132)mg/g、(5.618±0.125)mg/g、(3.293±0.046) mg/g、(3.285±0.049)mg/g、(0.583±0.021)mg/g、(0.565±0.023)mg/g,与对照组相比均明显减少,差异有统计学意义(P＜0.05).结论 DBP 850mg·kg-1·d-1在大鼠孕晚期染毒能高效诱导子代雄性大鼠发生ARMs,并对其生长发育产生明显不良影响.
目的 研究鄰苯二甲痠二丁酯(DBP)在大鼠孕晚期染毒誘導子代雄性大鼠髮生肛門直腸畸形(ARMs),併探討DBP對子代雄性大鼠生長髮育的影響.方法 孕鼠20隻,隨機分為兩組,懷孕第12～18天,每天分彆予大豆油及DBP 850 mg/kg灌胃.齣生後(PND) 1d統計肛門閉鎖髮生率,測量雄性仔鼠體重和肛門生殖器距離(AGD).PND 7 d觀察肛門閉鎖雄性仔鼠末耑直腸及肛週組織的病理學改變.PND 35 d統計雄性仔鼠體重、體長(除尾)和腹圍,解剖後對實質髒器(腦、心、肝、脾、肺、腎)以及生殖器官(睪汍、附睪)稱重,計算髒器繫數,評估髮育情況.結果 雄性仔鼠肛門閉鎖髮生率為39.5％.PND 1d肛門閉鎖雄性仔鼠體重及AGD分彆為(4.876±0.067)g、(2.401±0.341)mm,對照組分彆為(6.365±0.085)g、(2.993±0.403)mm,與對照組相比明顯降低,差異有統計學意義(P＜0.05).PND 7 d末耑直腸及肛週組織病理顯示典型的ARMs.PND 35 d肛門閉鎖雄性仔鼠腹圍及體長分彆為(20.021±0.416) mm、(12.925±0.158)mm,對照組分彆為(11.993±0.339)g、(14.943±0.171)mm,與對照組相比,腹圍增加且體長縮短,差異有統計學意義(P＜0.05).解剖後髮現,肛門閉鎖雄性仔鼠均髮生巨結腸改變,實質髒器(腦、心、肝、脾、肺、左腎、右腎)以及生殖器官(左睪汍、右睪汍、左附睪、右附睪)髒器繫數分彆為(9.312±0.233)mg/g、(3.541±0.079) mg/g、(46.382±1.309)mg/g、(3.653±0.098) mg/g、(8.872±0.323)mg/g、(5.261±0.108) mg/g、(5.093±0.096)mg/g、(1.725±0.049) mg/g、(1.513±0.041) mg/g、(0.169±0.009) mg/g、(0.182±0.008) mg/g,對照組分彆為(12.452±0.213)mg/g、(4.582±0.128)mg/g、(52.853±1.256) mg/g、(4.012±0.145)mg/g、(10.079±0.292)mg/g、(5.843±0.132)mg/g、(5.618±0.125)mg/g、(3.293±0.046) mg/g、(3.285±0.049)mg/g、(0.583±0.021)mg/g、(0.565±0.023)mg/g,與對照組相比均明顯減少,差異有統計學意義(P＜0.05).結論 DBP 850mg·kg-1·d-1在大鼠孕晚期染毒能高效誘導子代雄性大鼠髮生ARMs,併對其生長髮育產生明顯不良影響.
목적 연구린분이갑산이정지(DBP)재대서잉만기염독유도자대웅성대서발생항문직장기형(ARMs),병탐토DBP대자대웅성대서생장발육적영향.방법 잉서20지,수궤분위량조,부잉제12～18천,매천분별여대두유급DBP 850 mg/kg관위.출생후(PND) 1d통계항문폐쇄발생솔,측량웅성자서체중화항문생식기거리(AGD).PND 7 d관찰항문폐쇄웅성자서말단직장급항주조직적병이학개변.PND 35 d통계웅성자서체중、체장(제미)화복위,해부후대실질장기(뇌、심、간、비、폐、신)이급생식기관(고환、부고)칭중,계산장기계수,평고발육정황.결과 웅성자서항문폐쇄발생솔위39.5％.PND 1d항문폐쇄웅성자서체중급AGD분별위(4.876±0.067)g、(2.401±0.341)mm,대조조분별위(6.365±0.085)g、(2.993±0.403)mm,여대조조상비명현강저,차이유통계학의의(P＜0.05).PND 7 d말단직장급항주조직병리현시전형적ARMs.PND 35 d항문폐쇄웅성자서복위급체장분별위(20.021±0.416) mm、(12.925±0.158)mm,대조조분별위(11.993±0.339)g、(14.943±0.171)mm,여대조조상비,복위증가차체장축단,차이유통계학의의(P＜0.05).해부후발현,항문폐쇄웅성자서균발생거결장개변,실질장기(뇌、심、간、비、폐、좌신、우신)이급생식기관(좌고환、우고환、좌부고、우부고)장기계수분별위(9.312±0.233)mg/g、(3.541±0.079) mg/g、(46.382±1.309)mg/g、(3.653±0.098) mg/g、(8.872±0.323)mg/g、(5.261±0.108) mg/g、(5.093±0.096)mg/g、(1.725±0.049) mg/g、(1.513±0.041) mg/g、(0.169±0.009) mg/g、(0.182±0.008) mg/g,대조조분별위(12.452±0.213)mg/g、(4.582±0.128)mg/g、(52.853±1.256) mg/g、(4.012±0.145)mg/g、(10.079±0.292)mg/g、(5.843±0.132)mg/g、(5.618±0.125)mg/g、(3.293±0.046) mg/g、(3.285±0.049)mg/g、(0.583±0.021)mg/g、(0.565±0.023)mg/g,여대조조상비균명현감소,차이유통계학의의(P＜0.05).결론 DBP 850mg·kg-1·d-1재대서잉만기염독능고효유도자대웅성대서발생ARMs,병대기생장발육산생명현불량영향.
Objective To induce anorectal malformations (ARMs) in male rats by maternal exposure to di-n-butyl phthalate (DBP) during late pregnancy and examine the development abnormalities in male rats.Methods Twenty pregnant rats were randomly divided into 2 groups.DBP was administered by gastric intubation at adose of 850 mg/kg/d from gestation day (GD) 12 to 18.At postnatal day (PND) 1,body weight was measured and the incidence of ARMs and anogenital distance (AGD) were evaluated.At PND7,histological changes of ARMs were observed.At PND35,body weight,abdominal circumference and body length (with tail removal) were observed.The developmental abnormalities were also examined by evaluating the values of organ to body weight ratios of solid organs (brain,heart,liver,spleen,lung & kidney) and reproductive organs (testes & epididymis).Results The incidence of ARMs was 39.5％.Body weigh and AGD of ARMs at PND1 were (4.876 ± 0.067) g,(2.401 ± 0.341) mm versus (6.365 ± 0.085) g,(2.993 ± 0.403) mm in controls.The difference had statistical significance (P＜0.05).At PND7,histological analysis was conducted for con? rming the malformations.The abdominal circumference of ARMs signi? cantly increased at PND35 whereas body length (with tail removal) of ARMs were (20.021 ± 0.416),012.925 ± 0.158)mm versus (11.993 ±0.339),(14.943 ± 0.171) mm in controls.The differences had statistical significance (P＜0.05).Autopsy analysis of ARMs suggested secondary megacolon.The organ to body weight ratios of solid organs (brain,heart,liver,spleen,lung,left kidney & right kidney) and reproductive organs (left testicle,right testicle,left epididymis & right epididymis) were (9.312 ± 0.233),(3.541±0.079),(46.382±1.309),(3.653±0.098),(8.872±0.323),(5.261±0.108),(5.093 ± 0.096),(1.725 ± 0.049),(1.513 ± 0.041),(0.169 ± 0.009) and (0.182 ± 0.008) mg/g versus (12.452±0.213),(4.582± 0.128),(52.853 ± 1.256),(4.012± 0.145),(10.079 ± 0.292),(5.843 ± 0.132),(5.618 ± 0.125),(3.293 ± 0.046),(3.285 ± 0.049),(0.583 ± 0.021) and (0.565 ± 0.023) mg/g in controls.The differences had statistical significance (P＜0.05).Conclusions During late pregnancy,the exposure to DBP at dose of 850 mg/kg/d may lead to a high incidence of ARMs and developmental compromises.
