CAJ | 학술논문

目的研究成纤维细胞生长因子4 (FGF-4)在阿糖胞苷所致的拇指多指畸形鼠的肢芽中表达的变化;探索多指畸形的形成机制.方法运用腹腔注射阿糖胞苷的方法建立拇指多指畸形鼠的动物模型,选择不同给药时间的孕鼠(11.5 d、12.5d、13.5d、14.5 d),采用整体原位杂交的方法检测胚胎鼠肢芽的间充质细胞中FGF-4的表达;采用HE染色及软骨染色的方法检测胚胎鼠骨骼及软组织的发育情况;对正常出生的畸形鼠进行X线摄片,以进一步确定骨骼发育的畸形变化.结果在给药组所产生的鼠胚中,通过整体原位杂交方法检测,FGF4表达在给药组中11.5d、12.5d、13.5d和14.5d时FGF4表达的相对面积分别为22.21％、84.81％、58.43％和47.59％,而空白组中各天数的FGF4表达相对面积分别为6.74％、30.80％、19.64％和15.44％.FGF-4在肢芽的表达比正常组明显增多,其所分布的范围比对照组广,持续的时间比对照组长.HE染色和软骨染色发现拇指多指畸形鼠的肢体骨骼呈现多掌骨及多指骨的畸形变化.结论 FGF-4在阿糖胞苷所致的拇指多指畸形鼠的肢芽呈现高表达.
목적 연구성섬유세포생장인자4 (FGF-4)재아당포감소치적무지다지기형서적지아중표체적변화;탐색다지기형적형성궤제.방법 운용복강주사아당포감적방법건립무지다지기형서적동물모형,선택불동급약시간적잉서(11.5 d、12.5d、13.5d、14.5 d),채용정체원위잡교적방법검측배태서지아적간충질세포중FGF-4적표체;채용HE염색급연골염색적방법검측배태서골격급연조직적발육정황;대정상출생적기형서진행X선섭편,이진일보학정골격발육적기형변화.결과 재급약조소산생적서배중,통과정체원위잡교방법검측,FGF4표체재급약조중11.5d、12.5d、13.5d화14.5d시FGF4표체적상대면적분별위22.21％、84.81％、58.43％화47.59％,이공백조중각천수적FGF4표체상대면적분별위6.74％、30.80％、19.64％화15.44％.FGF-4재지아적표체비정상조명현증다,기소분포적범위비대조조엄,지속적시간비대조조장.HE염색화연골염색발현무지다지기형서적지체골격정현다장골급다지골적기형변화.결론 FGF-4재아당포감소치적무지다지기형서적지아정현고표체.
Objective To explore the expression and mechanisms of fibroblast growth factor 4 (FGF-4) in cytarabine-induced polydactyly of thumb.Methods An intraperitoneal injection of cytarabine was used to establish a rat model of polydactyl and syndactyl.For different periods of gestational rats (11.5,12.5,13.5,14.5 days),whole mount in situ hybridization was used to detect the expression of FGF-4 in polydactyly.The stains of hematoxylin & eosin and cartilage were used to detect the embryonic development of skeleton and tissues.And radiology was employed for detecting the aberration of skeleton.Results In test group,the expression of FGF-4 was up-regulated in limb bud and its expanded domain persisted longer than control group.In test group,the FGF4 expression relative areas at Days 11.5,12.5,13.5 and 14.5 were around 22.21％,84.81％,58.43％ and 47.59％ versus 6.74％,30.80％,19.64％ and 15.44％ respectively.Conclusions FGF-4 is up-regulated in cytarabine-induced limb bud of polydactyl embryos.