中华实验眼科杂志
中華實驗眼科雜誌
중화실험안과잡지
CHINESE JOURNAL OF EXPERIMENTAL OPHTHALMOLOGY
2012年
10期
893-896
,共4页
小牛血清去蛋白眼用凝胶%角膜碱烧伤%碱性成纤维细胞生长因子
小牛血清去蛋白眼用凝膠%角膜堿燒傷%堿性成纖維細胞生長因子
소우혈청거단백안용응효%각막감소상%감성성섬유세포생장인자
Deproteinised calf serum eye gel%Corneal alkali burn%Basic fibroblast growth factor
背景 角膜化学烧伤,特别是碱烧伤对眼组织造成了严重危害,目前临床上多采用抗炎、抑制免疫反应、角膜移植术等方法进行治疗,但针对角膜碱烧伤微环境下的组织修复研究也十分必要. 目的 观察角膜修复剂小牛血清去蛋白眼用凝胶对兔角膜碱烧伤的治疗作用. 方法 采用浸有0.5 mol/L NaOH溶液的滤纸贴附于角膜中央的方法制备兔眼角膜碱烧伤模型共24只兔24只眼,采用随机数字表法将模型眼随机分为生理盐水对照组、小牛血清去蛋白眼用凝胶组、空白凝胶基质组、碱性成纤维细胞生长因子(bFGF)阳性对照组4个组,分别采用生理盐水、小牛血清去蛋白眼用凝胶、凝胶空白基质和重组牛bFGF眼用凝胶点眼,每日4次,连续2周.分别于给药前及给药后3、5、7、10、14d于裂隙灯下观察各组眼的角膜溃疡和炎症反应,参照Ando等的评分标准对角膜炎症进行评分,进行角膜上皮荧光素钠染色,记录眼角膜溃疡面积的大小,并参照Trousdale评分标准对角膜溃疡进行评分.于造模后14d用过量麻醉法处死动物并摘除实验眼眼球,对角膜组织行常规组织病理学检查. 结果 造模后兔眼角膜混浊、水肿、灰白,造模成功率为100％.不同药物点眼后7d,裂隙灯下检查发现生理盐水对照组角膜呈瓷白色混浊,可见前房积脓；小牛血清去蛋白眼用凝胶组角膜溃疡愈合；空白凝胶基质组角膜上皮完整,但眼内结构窥不清；而bFGF阳性对照组角膜溃疡愈合,但可见新生血管.点眼后3、5、7、10、14d,小牛血清去蛋白眼用凝胶组及bFGF阳性对照组眼前节炎症评分均明显低于生理盐水对照组,差异均有统计学意义(P＜0.01)；小牛血清去蛋白眼用凝胶组眼前节炎症评分虽然稍低于bFGF阳性对照组,但二者的差异无统计学意义(P＞0.05)；与空白凝胶基质组相比,小牛血清去蛋白眼用凝胶组各时间点兔眼炎症评分值明显下降,差异均有统计学意义(P＜0.05).小牛血清去蛋白眼用凝胶组点眼后,角膜溃疡评分均明显下降,与生理盐水对照组相比,角膜溃疡评分的差异有统计学意义(P＜0.01),与bFGF阳性对照组比较,角膜溃疡评分有所下降,但差异无统计学意义(P＞0.05),与空白凝胶基质组比较,角膜溃疡评分明显下降,差异有统计学意义(P＜0.05).结论 小牛血清去蛋白眼用凝胶可减轻家兔角膜碱烧伤的眼前节炎症反应,促进角膜上皮愈合,其疗效优于bFGF.
揹景 角膜化學燒傷,特彆是堿燒傷對眼組織造成瞭嚴重危害,目前臨床上多採用抗炎、抑製免疫反應、角膜移植術等方法進行治療,但針對角膜堿燒傷微環境下的組織脩複研究也十分必要. 目的 觀察角膜脩複劑小牛血清去蛋白眼用凝膠對兔角膜堿燒傷的治療作用. 方法 採用浸有0.5 mol/L NaOH溶液的濾紙貼附于角膜中央的方法製備兔眼角膜堿燒傷模型共24隻兔24隻眼,採用隨機數字錶法將模型眼隨機分為生理鹽水對照組、小牛血清去蛋白眼用凝膠組、空白凝膠基質組、堿性成纖維細胞生長因子(bFGF)暘性對照組4箇組,分彆採用生理鹽水、小牛血清去蛋白眼用凝膠、凝膠空白基質和重組牛bFGF眼用凝膠點眼,每日4次,連續2週.分彆于給藥前及給藥後3、5、7、10、14d于裂隙燈下觀察各組眼的角膜潰瘍和炎癥反應,參照Ando等的評分標準對角膜炎癥進行評分,進行角膜上皮熒光素鈉染色,記錄眼角膜潰瘍麵積的大小,併參照Trousdale評分標準對角膜潰瘍進行評分.于造模後14d用過量痳醉法處死動物併摘除實驗眼眼毬,對角膜組織行常規組織病理學檢查. 結果 造模後兔眼角膜混濁、水腫、灰白,造模成功率為100％.不同藥物點眼後7d,裂隙燈下檢查髮現生理鹽水對照組角膜呈瓷白色混濁,可見前房積膿；小牛血清去蛋白眼用凝膠組角膜潰瘍愈閤；空白凝膠基質組角膜上皮完整,但眼內結構窺不清；而bFGF暘性對照組角膜潰瘍愈閤,但可見新生血管.點眼後3、5、7、10、14d,小牛血清去蛋白眼用凝膠組及bFGF暘性對照組眼前節炎癥評分均明顯低于生理鹽水對照組,差異均有統計學意義(P＜0.01)；小牛血清去蛋白眼用凝膠組眼前節炎癥評分雖然稍低于bFGF暘性對照組,但二者的差異無統計學意義(P＞0.05)；與空白凝膠基質組相比,小牛血清去蛋白眼用凝膠組各時間點兔眼炎癥評分值明顯下降,差異均有統計學意義(P＜0.05).小牛血清去蛋白眼用凝膠組點眼後,角膜潰瘍評分均明顯下降,與生理鹽水對照組相比,角膜潰瘍評分的差異有統計學意義(P＜0.01),與bFGF暘性對照組比較,角膜潰瘍評分有所下降,但差異無統計學意義(P＞0.05),與空白凝膠基質組比較,角膜潰瘍評分明顯下降,差異有統計學意義(P＜0.05).結論 小牛血清去蛋白眼用凝膠可減輕傢兔角膜堿燒傷的眼前節炎癥反應,促進角膜上皮愈閤,其療效優于bFGF.
배경 각막화학소상,특별시감소상대안조직조성료엄중위해,목전림상상다채용항염、억제면역반응、각막이식술등방법진행치료,단침대각막감소상미배경하적조직수복연구야십분필요. 목적 관찰각막수복제소우혈청거단백안용응효대토각막감소상적치료작용. 방법 채용침유0.5 mol/L NaOH용액적려지첩부우각막중앙적방법제비토안각막감소상모형공24지토24지안,채용수궤수자표법장모형안수궤분위생리염수대조조、소우혈청거단백안용응효조、공백응효기질조、감성성섬유세포생장인자(bFGF)양성대조조4개조,분별채용생리염수、소우혈청거단백안용응효、응효공백기질화중조우bFGF안용응효점안,매일4차,련속2주.분별우급약전급급약후3、5、7、10、14d우렬극등하관찰각조안적각막궤양화염증반응,삼조Ando등적평분표준대각막염증진행평분,진행각막상피형광소납염색,기록안각막궤양면적적대소,병삼조Trousdale평분표준대각막궤양진행평분.우조모후14d용과량마취법처사동물병적제실험안안구,대각막조직행상규조직병이학검사. 결과 조모후토안각막혼탁、수종、회백,조모성공솔위100％.불동약물점안후7d,렬극등하검사발현생리염수대조조각막정자백색혼탁,가견전방적농；소우혈청거단백안용응효조각막궤양유합；공백응효기질조각막상피완정,단안내결구규불청；이bFGF양성대조조각막궤양유합,단가견신생혈관.점안후3、5、7、10、14d,소우혈청거단백안용응효조급bFGF양성대조조안전절염증평분균명현저우생리염수대조조,차이균유통계학의의(P＜0.01)；소우혈청거단백안용응효조안전절염증평분수연초저우bFGF양성대조조,단이자적차이무통계학의의(P＞0.05)；여공백응효기질조상비,소우혈청거단백안용응효조각시간점토안염증평분치명현하강,차이균유통계학의의(P＜0.05).소우혈청거단백안용응효조점안후,각막궤양평분균명현하강,여생리염수대조조상비,각막궤양평분적차이유통계학의의(P＜0.01),여bFGF양성대조조비교,각막궤양평분유소하강,단차이무통계학의의(P＞0.05),여공백응효기질조비교,각막궤양평분명현하강,차이유통계학의의(P＜0.05).결론 소우혈청거단백안용응효가감경가토각막감소상적안전절염증반응,촉진각막상피유합,기료효우우bFGF.
Background Corneal chemical burns,especially hazards of alkali burn become increasingly prominent.Clinically,anti-inflammatory,immuno-suppression,corneal transplantation are the common treating method for corneal alkali burn.But the research of tissue repair under the microenvironment of corneal alkali burn is necessary.Objective The aim of this study was to investigate the therapeutic effect of deproteinised calf serum eye gel on the corneal alkali burn.Methods Alkali burn model of cornea was established on the right eyes by putting the filter paper with 0.5 mol/L NaOH on the center cornea for 1 minute in 24 white rabbits.The model rabbits were divided randomly into 4 groups.Normal saline solution,deproteinised calf serum eye gel,blank matrix gel or recombinant bovine basic fibroblast growth factor(rb-bFGF)eye-gel was topically administered 4 times per day for 14days in the 4 groups,respectively.The inflammatory reaction was examined under the slit lamp and scored based on Ando' s criteria.Corneal fluorescine staining was performed to calculate the corneal ulcer area and scored based on Trousdale' s criteria.Histopathological examination of corneas was performed on the fourtcenth day after experiment.The use of the experiment animals complied with ARVO Statement.Results Corneal edema and opacification were seen in the model eyes with the modeling successful rate 100％.On the seventh day after experiment,the severe ulcer of cornea and hypopyon appeared in the normal saline solution group.Corneal epithelium was intact but the intrarocular structure was invisible in the blank matrix gel group.In th(c) rb-bFCF group,corneal new vessels were seen,however,the corneal ulcer completely regrow in the deproteinised calf serum eye gel group.In 3,5,7,10 and 14days after examination,the corneal inflammatory scores were significantly lower in the deproteinised calf serum eye gel group and rb-bFGF group than those of the normal saline solution(P＜0.01).No significant difference was found in the inflammatory score between the deproteinised calf serum eye gel group and rb-bFGF group (P＞0.05) but was significantly lower than the blank gel matrix group (P ＜ 0.05).With respect to the corneal ulcer,the score was decreased in the deproteinised calf serum eye gel group compared with the normal saline solution group and blank gel matrix group (P ＜ 0.05).Howcver,no significant difference was found in the corneal ulcer score between the deproteinised calf serum eye gel group and rb-bFGF group in various time points (P＞ 0.05).Conclusions Deproteinised calf serum eye gel can promote the healing of corneal ulcer and remit the inflammatory response afler corneal alkali burns with a better effectiveness than rb-bFGF.
