中华实验眼科杂志
中華實驗眼科雜誌
중화실험안과잡지
CHINESE JOURNAL OF EXPERIMENTAL OPHTHALMOLOGY
2012年
12期
1122-1126
,共5页
褚利群%董宁%肖林%徐冰%刘晶
褚利群%董寧%肖林%徐冰%劉晶
저리군%동저%초림%서빙%류정
糖尿病视网膜病变%单核细胞趋化蛋白-1%巨噬细胞游走抑制因子%房水
糖尿病視網膜病變%單覈細胞趨化蛋白-1%巨噬細胞遊走抑製因子%房水
당뇨병시망막병변%단핵세포추화단백-1%거서세포유주억제인자%방수
Diabetic retinopathy%Monocyte chemotactic protein-1%Migration inhibitory factor%Aqueous humor
背景 研究发现,巨噬细胞和白细胞等炎性细胞参与糖尿病视网膜病变(DR)的发生发展,有多种细胞因子在DR的发生发展过程中发挥促进作用,但DR患者的房水及血清中单核细胞趋化蛋白-1(MCP-1)和巨噬细胞游走抑制因子(MIF)水平的变化与DR病程的关系尚不完全清楚. 目的 探讨2型糖尿病患者房水中MCP-1和MIF的水平与DR病程的关系. 方法 纳入2010年9月至2011年6月在北京世纪坛医院眼科和内分泌科确诊的2型糖尿病合并白内障并已行白内障超声乳化手术或超声乳化联合玻璃体切割术患者80例,根据眼底情况分为无DR(NDR)组20例、非增生型DR(NPDR)组38例和增生型DR(PDR)组22例,并收集同期的非糖尿病白内障手术患者即对照组26例,各组患者年龄、性别分布的差异均无统计学意义.所有患者于术中抽取未稀释的房水0.1 ml,分别采用ELISA法检测房水中MCP-1和MIF的质量浓度并进行组间比较. 结果 PDR组、NPDR组、NDR组、对照组房水中平均MCP-1的质量浓度分别为(1660.78±562.98)、(1463.26±623.41)、(686.76±186.16)、(494.35±148.59)ng/L,总体比较差异有统计学意义(F=37.968,P=0.000),对照组与NDR组以及NPDR组与PDR组房水中MCP-1的质量浓度比较差异均无统计学意义(P=0.169、0.117)；NDR组以及NPDR组与PDR组房水中MCP-1的质量浓度均明显高于对照组,差异均有统计学意义(P=0.000).PDR组、NPDR组、NDR组、对照组患者房水中平均MIF的质量浓度分别为(6.85±1.99)、(3.56±0.90)、(1.10±0.48)、(0.86±0.46)μg/L,总体比较差异有统计学意义(F=144.502,P=0.000)；对照组与NDR组房水中MIF的质量浓度比较差异无统计学意义(P=0.475)；其余各组间两两比较差异均有统计学意义(P=0.000).所有受检者房水中MCP-1与MIF质量浓度变化呈正相关(r=0.564,P=0.000).PDR组、NPDR组、NDR组血清中MCP-1和MIF质量浓度较对照组均有所增加,但4个组间MCP-1和MIF质量浓度的总体比较差异均无统计学意义(F=2.158、0.813,P＞0.05). 结论 糖尿病患者房水中MCP-1和MIF与DR的严重程度密切相关,MCP-1和MIF在DR的损伤机制中有协同作用.
揹景 研究髮現,巨噬細胞和白細胞等炎性細胞參與糖尿病視網膜病變(DR)的髮生髮展,有多種細胞因子在DR的髮生髮展過程中髮揮促進作用,但DR患者的房水及血清中單覈細胞趨化蛋白-1(MCP-1)和巨噬細胞遊走抑製因子(MIF)水平的變化與DR病程的關繫尚不完全清楚. 目的 探討2型糖尿病患者房水中MCP-1和MIF的水平與DR病程的關繫. 方法 納入2010年9月至2011年6月在北京世紀罈醫院眼科和內分泌科確診的2型糖尿病閤併白內障併已行白內障超聲乳化手術或超聲乳化聯閤玻璃體切割術患者80例,根據眼底情況分為無DR(NDR)組20例、非增生型DR(NPDR)組38例和增生型DR(PDR)組22例,併收集同期的非糖尿病白內障手術患者即對照組26例,各組患者年齡、性彆分佈的差異均無統計學意義.所有患者于術中抽取未稀釋的房水0.1 ml,分彆採用ELISA法檢測房水中MCP-1和MIF的質量濃度併進行組間比較. 結果 PDR組、NPDR組、NDR組、對照組房水中平均MCP-1的質量濃度分彆為(1660.78±562.98)、(1463.26±623.41)、(686.76±186.16)、(494.35±148.59)ng/L,總體比較差異有統計學意義(F=37.968,P=0.000),對照組與NDR組以及NPDR組與PDR組房水中MCP-1的質量濃度比較差異均無統計學意義(P=0.169、0.117)；NDR組以及NPDR組與PDR組房水中MCP-1的質量濃度均明顯高于對照組,差異均有統計學意義(P=0.000).PDR組、NPDR組、NDR組、對照組患者房水中平均MIF的質量濃度分彆為(6.85±1.99)、(3.56±0.90)、(1.10±0.48)、(0.86±0.46)μg/L,總體比較差異有統計學意義(F=144.502,P=0.000)；對照組與NDR組房水中MIF的質量濃度比較差異無統計學意義(P=0.475)；其餘各組間兩兩比較差異均有統計學意義(P=0.000).所有受檢者房水中MCP-1與MIF質量濃度變化呈正相關(r=0.564,P=0.000).PDR組、NPDR組、NDR組血清中MCP-1和MIF質量濃度較對照組均有所增加,但4箇組間MCP-1和MIF質量濃度的總體比較差異均無統計學意義(F=2.158、0.813,P＞0.05). 結論 糖尿病患者房水中MCP-1和MIF與DR的嚴重程度密切相關,MCP-1和MIF在DR的損傷機製中有協同作用.
배경 연구발현,거서세포화백세포등염성세포삼여당뇨병시망막병변(DR)적발생발전,유다충세포인자재DR적발생발전과정중발휘촉진작용,단DR환자적방수급혈청중단핵세포추화단백-1(MCP-1)화거서세포유주억제인자(MIF)수평적변화여DR병정적관계상불완전청초. 목적 탐토2형당뇨병환자방수중MCP-1화MIF적수평여DR병정적관계. 방법 납입2010년9월지2011년6월재북경세기단의원안과화내분비과학진적2형당뇨병합병백내장병이행백내장초성유화수술혹초성유화연합파리체절할술환자80례,근거안저정황분위무DR(NDR)조20례、비증생형DR(NPDR)조38례화증생형DR(PDR)조22례,병수집동기적비당뇨병백내장수술환자즉대조조26례,각조환자년령、성별분포적차이균무통계학의의.소유환자우술중추취미희석적방수0.1 ml,분별채용ELISA법검측방수중MCP-1화MIF적질량농도병진행조간비교. 결과 PDR조、NPDR조、NDR조、대조조방수중평균MCP-1적질량농도분별위(1660.78±562.98)、(1463.26±623.41)、(686.76±186.16)、(494.35±148.59)ng/L,총체비교차이유통계학의의(F=37.968,P=0.000),대조조여NDR조이급NPDR조여PDR조방수중MCP-1적질량농도비교차이균무통계학의의(P=0.169、0.117)；NDR조이급NPDR조여PDR조방수중MCP-1적질량농도균명현고우대조조,차이균유통계학의의(P=0.000).PDR조、NPDR조、NDR조、대조조환자방수중평균MIF적질량농도분별위(6.85±1.99)、(3.56±0.90)、(1.10±0.48)、(0.86±0.46)μg/L,총체비교차이유통계학의의(F=144.502,P=0.000)；대조조여NDR조방수중MIF적질량농도비교차이무통계학의의(P=0.475)；기여각조간량량비교차이균유통계학의의(P=0.000).소유수검자방수중MCP-1여MIF질량농도변화정정상관(r=0.564,P=0.000).PDR조、NPDR조、NDR조혈청중MCP-1화MIF질량농도교대조조균유소증가,단4개조간MCP-1화MIF질량농도적총체비교차이균무통계학의의(F=2.158、0.813,P＞0.05). 결론 당뇨병환자방수중MCP-1화MIF여DR적엄중정도밀절상관,MCP-1화MIF재DR적손상궤제중유협동작용.
Background Various studies have suggested that inflammatory factors such as leucocytes and macrophages are involved in the occurrence and development of diabetic retinopathy (DR),and many cytokines promote the occurrence of DR.However,the relationship of aqueous and serum monocyte chemotactic protein-1 (MCP-1) and macrophage migration inhibitory factor (MIF) change with DR is unclear.Objective This study was to investigate the effects of MCP-1 and MIF in aqueous and serum during DR development.Methods Eighty patients with type 2 diabetes were enrolled from Beijing Shijitan Hospital.These patients received phacoemulsification or phacoemulsification and vitrectomy from September,2010 to June,2011.Twenty-six cataract patients in the same stage (without diabetes) who underwent phacoemulsification surgery served as controls.According to the clinical stage of the DR,the diabetic patients were classified as the non-DR group (NDR) (20 eyes),non-proliferative DR group (NPDR) (38 eyes) and proliferative DR group (PDR) (22 eyes).Aqueous humour and periphery blood samples were collected during the operation to detect MCP-1 and MIF using enzyme-linked immnunosorbent assay (ELISA).Written informed consent was obtained from each subject before any relevant medical examination.Results The average aqueous MCP-1 levels were(1660.78±562.98),(1463.26± 623.41),(686.76±186.16) and(494.35±148.59) ng/L in the PDR group,NPDR group,NDR group and control group,respectively,showing a significant difference among the 4 groups (F=37.968,P=0.000).No significant differences were found in the aqueous MCP-1 levels between the control group and NDR group (P=0.169),or between the NPDR group and PDR group (P=0.117).However,the aqueous MCP-1 levels were significantly elevated in the PDR group,NPDR group and NDR group compared with the control group (P=0.000).The average aqueous MIF levels were (6.85±1.99),(3.56±0.90),(1.10±0.48) and (0.86 ± 0.46) μg/L,respectively,with significant differences among them (F =144.502,P =0.000).Multiple comparisons between groups were found to be significantly different (P =0.000) according to the LSD-t test,except between the control group and NDR group (P =0.475).A significant positive correlation was seen between the aqueous MCP-1 level and MCP-1 level in all study participants (r =0.564,P =0.000).However,serum levels of MCP-1 and MIF were not statistically significantly different among the 4 groups (F =2.158,P＞0.05;F =0.813,P＞0.05).Conclusions The increase of the aqueous MIF and MCP-1 levels is associated with the progression of diabetic retinopathy.The results suggest that MIF and MCP-1 promote the occurrence of DR.
