CAJ | 학술논문

背景不同早产儿个体之间对早产儿视网膜病变(ROP)发病易感性的差异较大,可能与血管内皮生长因子(VEGF)基因多态性有关. 目的研究ROP患儿VEGF基因多态性的表型,探讨VEGF基因多态性与ROP的关系.方法采用前瞻性系列病例对照研究设计,纳入2006年1月至2009年12月在湖南省儿童医院新生儿科住院的1～5期ROP患儿99例作为ROP组,另纳入80例同期未发生ROP的早产儿为对照无ROP组；在ROP组中将行激光或冷冻治疗的患儿39例作为治疗组,同期未经治疗ROP自发消退的患儿60例作为非治疗组.ROP组与无ROP组间、治疗组与非治疗组间的人口基线学特征比较差异均无统计学意义(P＞0.05).抽取各组患儿外周血2 ml以提取DNA,利用焦磷酸测序法进行VEGF-A +405、VEGF-A936基因的多态性研究. 结果 ROP组患儿与无ROP组患儿VEGF-A+ 405基因表型的差异均无统计学意义(P=0.071,0R=O.675,95％ CI=0.444-1.026).ROP组患儿与无ROP组患儿VEGF-A936基因表型的差异亦无统计学意义(P=0.118,OR=0.768,95％ CI为2.823～4.614),但治疗组与非治疗组VEGF-A+ 405,VEGF-A936基因多态性表型的差异均有统计学意义(VEGF-A +405:P＜0.01,OR=0.875,95％CI为5.239-14.024; VEGF-A936:P=0.000,OR=3.609,95％ CI为0.711～0.829). 结论 VEGF-A+ 405和VEGF-A936基因的多态性与ROP易感性无关,但与ROP预后有关.携带VEGF-A+405、VEGF-A936等位基因可能增加ROP进展的风险.
배경 불동조산인개체지간대조산인시망막병변(ROP)발병역감성적차이교대,가능여혈관내피생장인자(VEGF)기인다태성유관. 목적 연구ROP환인VEGF기인다태성적표형,탐토VEGF기인다태성여ROP적관계.방법 채용전첨성계렬병례대조연구설계,납입2006년1월지2009년12월재호남성인동의원신생인과주원적1～5기ROP환인99례작위ROP조,령납입80례동기미발생ROP적조산인위대조무ROP조；재ROP조중장행격광혹냉동치료적환인39례작위치료조,동기미경치료ROP자발소퇴적환인60례작위비치료조.ROP조여무ROP조간、치료조여비치료조간적인구기선학특정비교차이균무통계학의의(P＞0.05).추취각조환인외주혈2 ml이제취DNA,이용초린산측서법진행VEGF-A +405、VEGF-A936기인적다태성연구. 결과 ROP조환인여무ROP조환인VEGF-A+ 405기인표형적차이균무통계학의의(P=0.071,0R=O.675,95％ CI=0.444-1.026).ROP조환인여무ROP조환인VEGF-A936기인표형적차이역무통계학의의(P=0.118,OR=0.768,95％ CI위2.823～4.614),단치료조여비치료조VEGF-A+ 405,VEGF-A936기인다태성표형적차이균유통계학의의(VEGF-A +405:P＜0.01,OR=0.875,95％CI위5.239-14.024; VEGF-A936:P=0.000,OR=3.609,95％ CI위0.711～0.829). 결론 VEGF-A+ 405화VEGF-A936기인적다태성여ROP역감성무관,단여ROP예후유관.휴대VEGF-A+405、VEGF-A936등위기인가능증가ROP진전적풍험.
Background Statistic data revealed that different retinopathy of pre-term infants have different susceptibility to retinopathy of prematurity (ROP),which may be associated with polymorphism of vascular endothelial growth factor(VEGF) gene.Objective This study was to determine the association of polymorphisms of VEGF gene with the risk for ROP.Methods This research was approved by Ethics Committee of Hunan Children's Hospital,and written informed consent was obtained from the parents of patients.A prospective case-controlled study was designed.Ninety-nine ROP patients in Hunan Children' s Hospital and 88 pre-termed children without ROP were included from January,2006 to December,2009.Thirty-nine patients who received retinal photocoagulation or cryotherapy were included as the treatment group,and 60 untreated but spontaneously regressed ROP patients as the non-treatment group.No significant differences were seen in demography between with the ROP group and the without ROP group,or between the treatment group and the non-treatment group (all P＞0.05).2 mL of peripheral blood was collected for the extraction of DNA.Gene polymorphisms of VEGF-A+405 and VEGF-A936 were detected using the pyrosequencing method.Results No significant difference was found in the frequencies of the VEGF-A+405 gene polymorphisms between the ROP group and without ROP group (P =0.071,OR =0.675,95 ％ CI =0.444-1.026).Also no significant difference was found in the frequencies of the VEGF-A936 gene polymorphisms between with the ROP group and without the ROP group (P =0.118,OR =0.768,95 ％ CI=2.823-4.614).However,the frequencies of the VEGF-A+405 gene polymorphisms were significantly higher in the ROP treatment group than the non-treatment group (P＜0.01,OR--0.857,95 ％ CI =5.239-14.024),and VEGF-A936 gene polymorphisms was also significantly higher in the ROP treatment group than the non-treatment group (P =0.000,OR =3.609,95 ％ CI =0.711-0.829).Conclusions There is no association between the VEGF-A+405/VEGF-A936 single nucleotide polymorphism with the risk of ROP,but polymorphisms of VEGF gene may be correlated with the prognosis of ROP.The carrier of VEGF-A +405 /VEGF-A936 allele is more susceptible to ROP progression.