中华实验眼科杂志
中華實驗眼科雜誌
중화실험안과잡지
CHINESE JOURNAL OF EXPERIMENTAL OPHTHALMOLOGY
2013年
3期
288-291
,共4页
崔巍%孔德铭%路强%云丽娜
崔巍%孔德銘%路彊%雲麗娜
최외%공덕명%로강%운려나
青光眼/原发性闭角型青光眼%蒙古族%发病机制%凋亡%小梁细胞%流式细胞学
青光眼/原髮性閉角型青光眼%矇古族%髮病機製%凋亡%小樑細胞%流式細胞學
청광안/원발성폐각형청광안%몽고족%발병궤제%조망%소량세포%류식세포학
Glaucoma/primary angle closure glaucoma%Mongolian%Pathogenesis%Apoptosis%Trabecula cell%Flow cytometry
背景 研究表明,蒙古族原发性闭角型青光眼(PACG)的患病率较汉族人群高3.02倍,了解其发病原因及机制对于蒙古族人群PACG的预防和治疗有积极的意义. 目的 从小梁细胞凋亡的角度探讨蒙古族PACG的发病机制.方法 参照中华医学会眼科分会青光眼组1987年制定的诊断标准纳入在内蒙古自治区人民医院眼科接受治疗的蒙古族PACG患者32例32眼及汉族PACG患者40例40眼,另选取因眼外伤需摘除眼球的蒙古族正常人13例13眼及汉族正常人17例17眼作为对照.术前测量受检眼眼压.所有患者均于常规小梁切除手术时取小梁组织,采用annexinv-FITC/PI双标记染色,在FACS alibur型四色流式细胞仪上进行双参数分析,测定坏死、凋亡和正常细胞的百分率. 结果 蒙古族、汉族PACG组及蒙古族和汉族正常对照组眼压测量值分别为(35.97-±7.11)、(38.70±6.82)、(14.69±2.91)和(13.59±2.91) mmHg(1 mmHg=0.133 kPa),4个组间的总体比较差异有统计学意义(F=106.144,P=0.000),其中蒙古族PACG组和汉族PACG组的眼压均明显高于正常对照组,差异有统计学意义(P＜0.05).蒙古族PACG组、汉族PACG组及蒙古族正常对照组、汉族正常对照组小梁细胞凋亡率分别为(7.14±0.67)％、(5.40±0.69)％、(5.86±0.91)％和(2.29±0.65)％,总体差异有统计学意义(F=174.888,P=0.000),其中蒙古族PACG组小梁细胞凋亡率明显高于汉族PACG组和蒙古族正常对照组,差异有统计学意义(P＜0.05).蒙古族PACG组和汉族PACG组比较、蒙古族正常对照组和汉族正常对照组间小梁细胞的凋亡率比较差异均无统计学意义(P＞0.05).小梁细胞凋亡率随着眼压的升高而增加(b=0.990,F=10.209,P=0.009),直线回归方程为Y=2.788±0.092X.结论 蒙古族PACG人群小梁细胞凋亡率明显高于汉族人群,是否与蒙古族PACG发病率高有关值得进一步研究.
揹景 研究錶明,矇古族原髮性閉角型青光眼(PACG)的患病率較漢族人群高3.02倍,瞭解其髮病原因及機製對于矇古族人群PACG的預防和治療有積極的意義. 目的 從小樑細胞凋亡的角度探討矇古族PACG的髮病機製.方法 參照中華醫學會眼科分會青光眼組1987年製定的診斷標準納入在內矇古自治區人民醫院眼科接受治療的矇古族PACG患者32例32眼及漢族PACG患者40例40眼,另選取因眼外傷需摘除眼毬的矇古族正常人13例13眼及漢族正常人17例17眼作為對照.術前測量受檢眼眼壓.所有患者均于常規小樑切除手術時取小樑組織,採用annexinv-FITC/PI雙標記染色,在FACS alibur型四色流式細胞儀上進行雙參數分析,測定壞死、凋亡和正常細胞的百分率. 結果 矇古族、漢族PACG組及矇古族和漢族正常對照組眼壓測量值分彆為(35.97-±7.11)、(38.70±6.82)、(14.69±2.91)和(13.59±2.91) mmHg(1 mmHg=0.133 kPa),4箇組間的總體比較差異有統計學意義(F=106.144,P=0.000),其中矇古族PACG組和漢族PACG組的眼壓均明顯高于正常對照組,差異有統計學意義(P＜0.05).矇古族PACG組、漢族PACG組及矇古族正常對照組、漢族正常對照組小樑細胞凋亡率分彆為(7.14±0.67)％、(5.40±0.69)％、(5.86±0.91)％和(2.29±0.65)％,總體差異有統計學意義(F=174.888,P=0.000),其中矇古族PACG組小樑細胞凋亡率明顯高于漢族PACG組和矇古族正常對照組,差異有統計學意義(P＜0.05).矇古族PACG組和漢族PACG組比較、矇古族正常對照組和漢族正常對照組間小樑細胞的凋亡率比較差異均無統計學意義(P＞0.05).小樑細胞凋亡率隨著眼壓的升高而增加(b=0.990,F=10.209,P=0.009),直線迴歸方程為Y=2.788±0.092X.結論 矇古族PACG人群小樑細胞凋亡率明顯高于漢族人群,是否與矇古族PACG髮病率高有關值得進一步研究.
배경 연구표명,몽고족원발성폐각형청광안(PACG)적환병솔교한족인군고3.02배,료해기발병원인급궤제대우몽고족인군PACG적예방화치료유적겁적의의. 목적 종소량세포조망적각도탐토몽고족PACG적발병궤제.방법 삼조중화의학회안과분회청광안조1987년제정적진단표준납입재내몽고자치구인민의원안과접수치료적몽고족PACG환자32례32안급한족PACG환자40례40안,령선취인안외상수적제안구적몽고족정상인13례13안급한족정상인17례17안작위대조.술전측량수검안안압.소유환자균우상규소량절제수술시취소량조직,채용annexinv-FITC/PI쌍표기염색,재FACS alibur형사색류식세포의상진행쌍삼수분석,측정배사、조망화정상세포적백분솔. 결과 몽고족、한족PACG조급몽고족화한족정상대조조안압측량치분별위(35.97-±7.11)、(38.70±6.82)、(14.69±2.91)화(13.59±2.91) mmHg(1 mmHg=0.133 kPa),4개조간적총체비교차이유통계학의의(F=106.144,P=0.000),기중몽고족PACG조화한족PACG조적안압균명현고우정상대조조,차이유통계학의의(P＜0.05).몽고족PACG조、한족PACG조급몽고족정상대조조、한족정상대조조소량세포조망솔분별위(7.14±0.67)％、(5.40±0.69)％、(5.86±0.91)％화(2.29±0.65)％,총체차이유통계학의의(F=174.888,P=0.000),기중몽고족PACG조소량세포조망솔명현고우한족PACG조화몽고족정상대조조,차이유통계학의의(P＜0.05).몽고족PACG조화한족PACG조비교、몽고족정상대조조화한족정상대조조간소량세포적조망솔비교차이균무통계학의의(P＞0.05).소량세포조망솔수착안압적승고이증가(b=0.990,F=10.209,P=0.009),직선회귀방정위Y=2.788±0.092X.결론 몽고족PACG인군소량세포조망솔명현고우한족인군,시부여몽고족PACG발병솔고유관치득진일보연구.
Background Research showed that the morbidity rate of primary angle closure glaucoma (PACG) in Mongolian population is 3.02 times more than Han nationality population.To understand the cause and mechanism of PACG in Mongolia is of an important significance.Objective This study was to investigate the pathogenesis of Mongolian PACG.Methods Thirty-two eyes of 32 PACG patients in Mongolia and 40 eyes of 40 PACG patients of Han peoples were included in Inner Mongolia Autonomous Region People's Hospital according to the diagnosis criteria of glaucoma group of Chinese Medical Ophthalmology Association (version 1987),and 13 eyes of 13 normal Mongolia and 17 eyes of 17 normal Han peoples who suffered with ocular truma were recruited as controls.Intraocular pressure(IOP) was measured before surgery.The trabecular meshwork tissue was obtained from all the eyes during the operation.Annexinv-FITC/PI double staining was performed and the apoptosis rate of trabecula cells was tested with flow cytometry.Written informed consent was obtained initial of the study.Results The IOP value in Mongolia PACG group,Han PACG group,Mengolia normal group and Han normal group was (35.97±7.11)mmHg,(38.70± 6.82) mmHg,(14.69 ± 2.91) mmHg and (13.59 ± 2.91) mmHg,respectively,showing a significant difference among the 4 groups(F=106.144,P=0.000),and the IOP was significantly higher in the Mengolia PACG group and Han PACG group than the normal groups(P＜0.05).The apoptosis rate of the cells was (7.14±0.67)％,(5.40±0.69) ％,(5.86±0.91) ％ and(2.29±0.65) ％ in the Mongolia PACG group,Han PACG group,Mongolia normal group and Han normal group,respectively,with a significant difference among them (F =174.888,P =0.000),and apoptosis rate of the Mongolia PACG group was significantly higher than that of the Han PACG group and the Mongolia normal group (P＜0.05).No significant difference was found between the Mongolia PACG group and the Han PACG group or between the Mongolia normal group and Han normal group (P＞0.05).The cell apoptosis rate was increased with the elevation of IOP (b =0.990,F=10.209,P =0.009) with the regression equition Y =2.788 +0.092X.Conclusions The apoptosis rate of trabecula cells in Mongolian is higher than Han people.If these results are associated with the high incidence of Mengolia PACG is worth of study.
