中华实验眼科杂志
中華實驗眼科雜誌
중화실험안과잡지
CHINESE JOURNAL OF EXPERIMENTAL OPHTHALMOLOGY
2013年
7期
625-629
,共5页
核质双向转运受体蛋白%IPO13%p63%角膜缘%肿瘤%干细胞%标志物
覈質雙嚮轉運受體蛋白%IPO13%p63%角膜緣%腫瘤%榦細胞%標誌物
핵질쌍향전운수체단백%IPO13%p63%각막연%종류%간세포%표지물
Nucleus-cytoplasm transport receptor protein%Importin13%p63%Limbus%Neoplasms%Stem cell%Marker
背景 正常干细胞相关标志物的发现为肿瘤发生机制的研究及寻找特异性的肿瘤干细胞标志物提供了新的思路,importin13(IPO13)是新发现的IPO β家族的成员,是该家族中惟一具有细胞核和细胞质双向转运功能的蛋白,其在眼部及角膜缘肿瘤组织中生物学特性的研究甚少. 目的 研究细胞核及细胞质双向转运受体蛋白IPO13在人角膜缘肿瘤中的表达,并与角膜缘干细胞标志物p63的表达进行比较,探讨两者在人角膜缘肿瘤中的表达差异及意义.方法 收集正常人供体眼球角膜缘处的结膜及角膜缘组织、角膜结膜乳头状瘤(CCP)和角膜结膜上皮内上皮癌(CCIN)组织标本各6例,采用冰冻切片免疫组织化学染色法检测相应组织中IPO13、p63的表达量(A值).采用单因素方差分析法和SNK-q检验进行统计学比较. 结果 IPO13主要表达于人正常眼球结膜、角膜缘上皮基底细胞的细胞核内,表达于CCP、CCIN组织上皮基底细胞和基底上层细胞的细胞核内,呈棕褐色颗粒.IPO13蛋白在正常角膜结膜、CCP、CCIN组织中的表达量(A值)分别为1687±1014、3546±1375和7635±2854,总体差异有统计学意义(F=7.23,P＜0.05),其中CCP、CCIN组织中的表达量明显高于正常角膜结膜组织,差异均有统计学意义(q=4.02、5.13,P＜0.05)；CCIN组织中的表达量明显高于CCP组织,差异有统计学意义(q=3.45,P＜0.05).p63在正常人眼球结膜、角膜缘、CCP组织上皮基底细胞和基底上层细胞的细胞核内及CCIN组织上皮全层的细胞核内均有表达.p63在正常角膜结膜组织中、CCP、CCIN组织中的表达量分别为2110±1229、3966±2129和6650±2136,差异有统计学意义(F=6.17,P＜0.05),其中CCP、CCIN标本中阳性表达的A值均明显高于正常角膜结膜标本(q=4.33、5.01,P＜0.05),CCIN标本中阳性表达的A值明显高于CCP标本,差异有统计学意义(q=3.83,P＜0.05). 结论 IPO13在角膜缘上皮增生性病变中的低分化细胞中作为标志物较p63更具特异性.与正常角膜结膜组织相比,IPO13与p63在CCP、CCIN组织中的表达逐步上调,可能在角膜结膜增生性病变中起到正性调控作用.IPO13与p63在良性与恶性角膜缘上皮增生性病变中的表达差异明显,提示IPO13与p63可能在角膜缘上皮增生性病变的异常增生与分化中起重要的调控作用.
揹景 正常榦細胞相關標誌物的髮現為腫瘤髮生機製的研究及尋找特異性的腫瘤榦細胞標誌物提供瞭新的思路,importin13(IPO13)是新髮現的IPO β傢族的成員,是該傢族中惟一具有細胞覈和細胞質雙嚮轉運功能的蛋白,其在眼部及角膜緣腫瘤組織中生物學特性的研究甚少. 目的 研究細胞覈及細胞質雙嚮轉運受體蛋白IPO13在人角膜緣腫瘤中的錶達,併與角膜緣榦細胞標誌物p63的錶達進行比較,探討兩者在人角膜緣腫瘤中的錶達差異及意義.方法 收集正常人供體眼毬角膜緣處的結膜及角膜緣組織、角膜結膜乳頭狀瘤(CCP)和角膜結膜上皮內上皮癌(CCIN)組織標本各6例,採用冰凍切片免疫組織化學染色法檢測相應組織中IPO13、p63的錶達量(A值).採用單因素方差分析法和SNK-q檢驗進行統計學比較. 結果 IPO13主要錶達于人正常眼毬結膜、角膜緣上皮基底細胞的細胞覈內,錶達于CCP、CCIN組織上皮基底細胞和基底上層細胞的細胞覈內,呈棕褐色顆粒.IPO13蛋白在正常角膜結膜、CCP、CCIN組織中的錶達量(A值)分彆為1687±1014、3546±1375和7635±2854,總體差異有統計學意義(F=7.23,P＜0.05),其中CCP、CCIN組織中的錶達量明顯高于正常角膜結膜組織,差異均有統計學意義(q=4.02、5.13,P＜0.05)；CCIN組織中的錶達量明顯高于CCP組織,差異有統計學意義(q=3.45,P＜0.05).p63在正常人眼毬結膜、角膜緣、CCP組織上皮基底細胞和基底上層細胞的細胞覈內及CCIN組織上皮全層的細胞覈內均有錶達.p63在正常角膜結膜組織中、CCP、CCIN組織中的錶達量分彆為2110±1229、3966±2129和6650±2136,差異有統計學意義(F=6.17,P＜0.05),其中CCP、CCIN標本中暘性錶達的A值均明顯高于正常角膜結膜標本(q=4.33、5.01,P＜0.05),CCIN標本中暘性錶達的A值明顯高于CCP標本,差異有統計學意義(q=3.83,P＜0.05). 結論 IPO13在角膜緣上皮增生性病變中的低分化細胞中作為標誌物較p63更具特異性.與正常角膜結膜組織相比,IPO13與p63在CCP、CCIN組織中的錶達逐步上調,可能在角膜結膜增生性病變中起到正性調控作用.IPO13與p63在良性與噁性角膜緣上皮增生性病變中的錶達差異明顯,提示IPO13與p63可能在角膜緣上皮增生性病變的異常增生與分化中起重要的調控作用.
배경 정상간세포상관표지물적발현위종류발생궤제적연구급심조특이성적종류간세포표지물제공료신적사로,importin13(IPO13)시신발현적IPO β가족적성원,시해가족중유일구유세포핵화세포질쌍향전운공능적단백,기재안부급각막연종류조직중생물학특성적연구심소. 목적 연구세포핵급세포질쌍향전운수체단백IPO13재인각막연종류중적표체,병여각막연간세포표지물p63적표체진행비교,탐토량자재인각막연종류중적표체차이급의의.방법 수집정상인공체안구각막연처적결막급각막연조직、각막결막유두상류(CCP)화각막결막상피내상피암(CCIN)조직표본각6례,채용빙동절편면역조직화학염색법검측상응조직중IPO13、p63적표체량(A치).채용단인소방차분석법화SNK-q검험진행통계학비교. 결과 IPO13주요표체우인정상안구결막、각막연상피기저세포적세포핵내,표체우CCP、CCIN조직상피기저세포화기저상층세포적세포핵내,정종갈색과립.IPO13단백재정상각막결막、CCP、CCIN조직중적표체량(A치)분별위1687±1014、3546±1375화7635±2854,총체차이유통계학의의(F=7.23,P＜0.05),기중CCP、CCIN조직중적표체량명현고우정상각막결막조직,차이균유통계학의의(q=4.02、5.13,P＜0.05)；CCIN조직중적표체량명현고우CCP조직,차이유통계학의의(q=3.45,P＜0.05).p63재정상인안구결막、각막연、CCP조직상피기저세포화기저상층세포적세포핵내급CCIN조직상피전층적세포핵내균유표체.p63재정상각막결막조직중、CCP、CCIN조직중적표체량분별위2110±1229、3966±2129화6650±2136,차이유통계학의의(F=6.17,P＜0.05),기중CCP、CCIN표본중양성표체적A치균명현고우정상각막결막표본(q=4.33、5.01,P＜0.05),CCIN표본중양성표체적A치명현고우CCP표본,차이유통계학의의(q=3.83,P＜0.05). 결론 IPO13재각막연상피증생성병변중적저분화세포중작위표지물교p63경구특이성.여정상각막결막조직상비,IPO13여p63재CCP、CCIN조직중적표체축보상조,가능재각막결막증생성병변중기도정성조공작용.IPO13여p63재량성여악성각막연상피증생성병변중적표체차이명현,제시IPO13여p63가능재각막연상피증생성병변적이상증생여분화중기중요적조공작용.
Background The study on normal stem cell markers provides a new way of thinking of that pathogenesis of cancer research and looking for specific markers of cancer stem cells.Importin13 (IPO13) is a novel nucleus-cytoplasm transport receptor protein of importin β family,the study on the biological behavior of IPO13 in ocular tissue and limbal neoplasms is lacking.Objective This study was to investigate the differential expression of IPO13 and p63 in human benign and malignant conjunctiva-cornea neoplasms.Methods The specimens of normal donor limbal and conjunctival tissues (6),conjunciva-cornea papilloma (CCP) (6) and conjunctiva-cornea intraepithelial neoplasia(CCIN) (6)were collected from Xiangya Hospital of Center South University.The expressions of IPO13 and p63 in the corresponding tissue were qualitatively and quantitatively detected using immunochemistry.The A values of IPO13 and p63 positive response were calculated and compared among the 3 types specimens.Results The immunohistostaining on frozen sections showed that IPO13 was expressed in nuclei of basal cells of limbal and conjunctival epithelium and the cellular nuclei of basal cells and suprabasal cells of CCP and CCIN epithelium.The A values of IPO13 positive expression were 1687± 1014,3546± 1375 and 7635 ±2854 in the normal limbal specimen,CCP and CCIN,respectively,showing a significant difference among them (F =7.23,P＜0.05),and the A value was higher in the CCP and CCIN than that in the normal limbal tissue (q =4.02,5.13,P＜0.05),and that in the CCIN was significantly elevated in comparison with CCP(q =3.45,P＜0.05).p63 was expressed in nuclei of basal cells and suprabasal of limbal,conjunctival and CCP epithelium,and was expressed in nuclei of the entire CCIN epithelium.The expressions of p63 in the normal conjunctiva-cornea tissue,CCP and CCIN were 2110± 1229,3966± 2129 and 6650± 2136 respectively,with significant difference among the three different specimens (F =6.17,P＜ 0.05),and the A value of p63 positive expression was significantly elevated in the CCP and CCIN compared with normal limbal specimen (q =4.33,5.01,P＜0.05),and that in the CCIN was significantly elevated in comparison with CCP(q=3.83,P＜0.05).Conclusions IPO13 is more specifical in marking the poorly differentiated cells in limbal epithelial proliferative lesions than p63.Compared with the normal limbal specimen,the expressions of IPO13 and p63 in CCP and CCIN specimens gradually upregulat,which suggests that IPO13 and p63 may play a positive regulatory role in conjunctiva-cornea proliferative lesions.The differential expression of IPO13 and p63 is predominant between benign and malignant limbal epithelial proliferative lesions,indicating that IPO13 and p63 may play an important role in regulating the abnormal proliferation and differentiation of limbal epithelial proliferative lesions.
