中华实验眼科杂志
中華實驗眼科雜誌
중화실험안과잡지
CHINESE JOURNAL OF EXPERIMENTAL OPHTHALMOLOGY
2014年
6期
502-505
,共4页
段海霞%曹志星%管怀进%张天一
段海霞%曹誌星%管懷進%張天一
단해하%조지성%관부진%장천일
晶状体%上皮细胞%锥虫蓝-茜素红染色%苏木精-伊红染色%超微结构
晶狀體%上皮細胞%錐蟲藍-茜素紅染色%囌木精-伊紅染色%超微結構
정상체%상피세포%추충람-천소홍염색%소목정-이홍염색%초미결구
Lens%Epithelial cell%Trypan blue and alizarin red stain%Haematoxylin and eosin stain%Ultrastructure
背景 白内障的发生与晶状体上皮细胞(LECs)结构和功能的受损有直接关系,而白内障LECs的特征性形态学变化是目前证实细胞结构和功能受损最有力的证据,了解不同类型白内障的LECs形态变化对于研究不同环境或不同疾病对LECs的生物学行为有重要意义. 目的 观察不同类型白内障LECs的形态学变化.方法 在白内障摘出手术过程中分别收集年龄相关性白内障、糖尿病性白内障及高度近视并发性白内障的晶状体前囊膜各15片,各种类型白内障的前囊膜分别进行锥虫蓝-茜素红(TB-AR)染色和苏木精-伊红染色,评估LECs的活性和形态,并在透射电子显微镜下观察各种类型白内障LECs的超微结构改变,对不同类型白内障的LECs生物学行为进行比较.结果 TB-AR染色结果表明,年龄相关性白内障LECs大小不等,呈多边形,镶嵌样排列,少量细胞死亡,细胞膜模糊不清,细胞核呈圆形;糖尿病性白内障LECs水肿,体积增大,细胞大小不等;而高度近视并发白内障LECs体积较小,大部分细胞死亡.苏木精-伊红染色显示,年龄相关性白内障晶状体囊膜为一均质膜,LECs层呈单层排列,多数细胞结构完整,紧密贴附于前囊膜上;糖尿病性白内障前囊膜部分区域LECs与囊膜间有空隙,细胞核大小不等;高度近视并发性白内障LECs较小,形态不规则,细胞着色深.透射电子显微镜下观察发现,年龄相关性白内障前囊膜及多数LECs形态正常,细胞间以及细胞与囊膜间连接尚可;糖尿病性白内障LECs之间以及细胞与囊膜间也可见紧密连接,但显示细胞水肿,细胞间隙扩大,高度近视并发性白内障LECs可见细胞质嵌合突起,细胞质内有空泡变性.结论 年龄相关性白内障、糖尿病性白内障和高度近视并发性白内障均以LECs的变性、坏死、凋亡为共同的细胞学基础,但糖尿病性白内障和高度近视并发性白内障的LECs改变明显重于年龄相关性白内障.
揹景 白內障的髮生與晶狀體上皮細胞(LECs)結構和功能的受損有直接關繫,而白內障LECs的特徵性形態學變化是目前證實細胞結構和功能受損最有力的證據,瞭解不同類型白內障的LECs形態變化對于研究不同環境或不同疾病對LECs的生物學行為有重要意義. 目的 觀察不同類型白內障LECs的形態學變化.方法 在白內障摘齣手術過程中分彆收集年齡相關性白內障、糖尿病性白內障及高度近視併髮性白內障的晶狀體前囊膜各15片,各種類型白內障的前囊膜分彆進行錐蟲藍-茜素紅(TB-AR)染色和囌木精-伊紅染色,評估LECs的活性和形態,併在透射電子顯微鏡下觀察各種類型白內障LECs的超微結構改變,對不同類型白內障的LECs生物學行為進行比較.結果 TB-AR染色結果錶明,年齡相關性白內障LECs大小不等,呈多邊形,鑲嵌樣排列,少量細胞死亡,細胞膜模糊不清,細胞覈呈圓形;糖尿病性白內障LECs水腫,體積增大,細胞大小不等;而高度近視併髮白內障LECs體積較小,大部分細胞死亡.囌木精-伊紅染色顯示,年齡相關性白內障晶狀體囊膜為一均質膜,LECs層呈單層排列,多數細胞結構完整,緊密貼附于前囊膜上;糖尿病性白內障前囊膜部分區域LECs與囊膜間有空隙,細胞覈大小不等;高度近視併髮性白內障LECs較小,形態不規則,細胞著色深.透射電子顯微鏡下觀察髮現,年齡相關性白內障前囊膜及多數LECs形態正常,細胞間以及細胞與囊膜間連接尚可;糖尿病性白內障LECs之間以及細胞與囊膜間也可見緊密連接,但顯示細胞水腫,細胞間隙擴大,高度近視併髮性白內障LECs可見細胞質嵌閤突起,細胞質內有空泡變性.結論 年齡相關性白內障、糖尿病性白內障和高度近視併髮性白內障均以LECs的變性、壞死、凋亡為共同的細胞學基礎,但糖尿病性白內障和高度近視併髮性白內障的LECs改變明顯重于年齡相關性白內障.
배경 백내장적발생여정상체상피세포(LECs)결구화공능적수손유직접관계,이백내장LECs적특정성형태학변화시목전증실세포결구화공능수손최유력적증거,료해불동류형백내장적LECs형태변화대우연구불동배경혹불동질병대LECs적생물학행위유중요의의. 목적 관찰불동류형백내장LECs적형태학변화.방법 재백내장적출수술과정중분별수집년령상관성백내장、당뇨병성백내장급고도근시병발성백내장적정상체전낭막각15편,각충류형백내장적전낭막분별진행추충람-천소홍(TB-AR)염색화소목정-이홍염색,평고LECs적활성화형태,병재투사전자현미경하관찰각충류형백내장LECs적초미결구개변,대불동류형백내장적LECs생물학행위진행비교.결과 TB-AR염색결과표명,년령상관성백내장LECs대소불등,정다변형,양감양배렬,소량세포사망,세포막모호불청,세포핵정원형;당뇨병성백내장LECs수종,체적증대,세포대소불등;이고도근시병발백내장LECs체적교소,대부분세포사망.소목정-이홍염색현시,년령상관성백내장정상체낭막위일균질막,LECs층정단층배렬,다수세포결구완정,긴밀첩부우전낭막상;당뇨병성백내장전낭막부분구역LECs여낭막간유공극,세포핵대소불등;고도근시병발성백내장LECs교소,형태불규칙,세포착색심.투사전자현미경하관찰발현,년령상관성백내장전낭막급다수LECs형태정상,세포간이급세포여낭막간련접상가;당뇨병성백내장LECs지간이급세포여낭막간야가견긴밀련접,단현시세포수종,세포간극확대,고도근시병발성백내장LECs가견세포질감합돌기,세포질내유공포변성.결론 년령상관성백내장、당뇨병성백내장화고도근시병발성백내장균이LECs적변성、배사、조망위공동적세포학기출,단당뇨병성백내장화고도근시병발성백내장적LECs개변명현중우년령상관성백내장.
Background Cataract was directly associated with the damage to the structure and function of lens epithelial cells (LECs).In those patients who suffer from cataracts,morphologic changes of LECs is the most compelling evidence confirming loss of cellular structure and function of LECs.So,learning about the morphological changes of LECs of the different types of cataracts is very important for study on biological behaviors of LECs in different environments or diseases.Objective This study was to evaluate the morphological and ultrastructural changes of the LECs in different types of cataracts.Methods Anterior capsular member from age-related cataracts,diabetic cataracts and high myopia complicated cataract were obtained during the cataract surgery and 15 pieces for each.Trypan blue and alizarin red (TB-AR) stain,haematoxylin and eosin stain were performed in the samples to assess the viability and morphology of LECs.The ultrastructural change of LECs was observed under the transmission electron microscope.The features of the LECs were compared among the different types of cataract.Results TB-AR stain showed that LECs were polygon in shape with the mosaic arrangement and round cell nucleus,and a few dead cells were seen in the samples age-related cataract.In the diabetic samples,LECs largened from swelling with different sizes.More dead cells were found in the high myopia complicated cataract.Haematoxylin and eosin stain exhibited that the anterior capsular membrane presented a homogeneuous membrane,and monolayer LECs attached firmly anterior capsular membrane in the samples of related cataract.Majority of the cells had the intact structure.However,the interspaces between cells and capsular membrane were found in diabetic cataract.Also,smaller LECs were seen in high myopia complicated cataract with the irregular cell morphology.Under the transmission electron microscope,LECs presented the normal shape,intact intercellular tight junction and well attachment between cells and capsular membrane in the samples of the age-related cataract.In the samples of the diabetic cataract,edema of LECs and large intercellular spaces were seen.In addition,the jogged pump and vacuolar degeneration of cytoplasm were revealed in the high myopia complicated cataract.Conclusions The degeneration,necrosis and apoptosis was a common pathological basis of age-related cataract,diabetic cataract and high myopia complicated cataract.However,the damage of LECs was more serious in diabetic cataract and high myopia complicated cataract than that of agerelated cataract.
