中华眼视光学与视觉科学杂志
中華眼視光學與視覺科學雜誌
중화안시광학여시각과학잡지
CHINESE JOURNAL OF OPTOMETRY OPHTHALMOLOGY AND VISUAL SCIENCE
2014年
7期
436-440
,共5页
涂姝%李康%黄晶晶%葛坚
塗姝%李康%黃晶晶%葛堅
도주%리강%황정정%갈견
恒河猴%动物模型%高眼压%视神经纤维
恆河猴%動物模型%高眼壓%視神經纖維
항하후%동물모형%고안압%시신경섬유
Rhesus monkey%Animal model%Ocular hypertension%Retinal nerve fiber layer
目的 观察恒河猴慢性高眼压模型与开角型青光眼患者的视乳头和视神经纤维损伤模式是否一致.方法 实验研究.采用眼科多波长激光仪光凝恒河猴(12眼)的小梁网以破坏小梁网功能使眼压升高,建立恒河猴慢性高眼压模型.术前和术后每周行眼压、眼底照相、视乳头参数和视神经纤维层厚度检测.结果 建模的12眼术前眼压为(16.08±2.02)mmHg,中央角膜厚度为(489.17±17.82)μm,眼轴为(20.32±0.84) mm.12只用于诱导建模的恒河猴眼,经3～4次激光光凝,11眼被成功诱导.27周平均眼压为(30.32±14.59)mmHg,眼压波动幅度为(8.19±7.45) mmHg.光学相关断层扫描成像显示整个盘沿面积从(1.67±0.44)mm2减小到(0.43±0.34)mm2.平均视神经纤维从(97.92±6.79)μm减少到(64.46±17.44)μm.结论 恒河猴慢性高眼压性模型模拟了人类高眼压/开角型青光眼发病机制和过程,在视杯对高眼压的反应、视杯可逆性缩小、视神经纤维损伤现象等方面一致,是开展高眼压性视乳头和视神经纤维层损伤研究理想的动物模型.
目的 觀察恆河猴慢性高眼壓模型與開角型青光眼患者的視乳頭和視神經纖維損傷模式是否一緻.方法 實驗研究.採用眼科多波長激光儀光凝恆河猴(12眼)的小樑網以破壞小樑網功能使眼壓升高,建立恆河猴慢性高眼壓模型.術前和術後每週行眼壓、眼底照相、視乳頭參數和視神經纖維層厚度檢測.結果 建模的12眼術前眼壓為(16.08±2.02)mmHg,中央角膜厚度為(489.17±17.82)μm,眼軸為(20.32±0.84) mm.12隻用于誘導建模的恆河猴眼,經3～4次激光光凝,11眼被成功誘導.27週平均眼壓為(30.32±14.59)mmHg,眼壓波動幅度為(8.19±7.45) mmHg.光學相關斷層掃描成像顯示整箇盤沿麵積從(1.67±0.44)mm2減小到(0.43±0.34)mm2.平均視神經纖維從(97.92±6.79)μm減少到(64.46±17.44)μm.結論 恆河猴慢性高眼壓性模型模擬瞭人類高眼壓/開角型青光眼髮病機製和過程,在視杯對高眼壓的反應、視杯可逆性縮小、視神經纖維損傷現象等方麵一緻,是開展高眼壓性視乳頭和視神經纖維層損傷研究理想的動物模型.
목적 관찰항하후만성고안압모형여개각형청광안환자적시유두화시신경섬유손상모식시부일치.방법 실험연구.채용안과다파장격광의광응항하후(12안)적소량망이파배소량망공능사안압승고,건립항하후만성고안압모형.술전화술후매주행안압、안저조상、시유두삼수화시신경섬유층후도검측.결과 건모적12안술전안압위(16.08±2.02)mmHg,중앙각막후도위(489.17±17.82)μm,안축위(20.32±0.84) mm.12지용우유도건모적항하후안,경3～4차격광광응,11안피성공유도.27주평균안압위(30.32±14.59)mmHg,안압파동폭도위(8.19±7.45) mmHg.광학상관단층소묘성상현시정개반연면적종(1.67±0.44)mm2감소도(0.43±0.34)mm2.평균시신경섬유종(97.92±6.79)μm감소도(64.46±17.44)μm.결론 항하후만성고안압성모형모의료인류고안압/개각형청광안발병궤제화과정,재시배대고안압적반응、시배가역성축소、시신경섬유손상현상등방면일치,시개전고안압성시유두화시신경섬유층손상연구이상적동물모형.
Objective To investigate if the chronic ocular hypertension (COHT) in a monkey model presents indistinguishable from humans in its retinal nerve fiber layer (RNFL) and optic nerve head (ONH) damage.Methods This was an experimental study.Models of COHT were induced in 12 eyes of rhesus monkeys were induced by using VISULAS Trion to disturb the aqueous humor outflow and cause the elevation of intraocular pressure.Some measurements have been taken such as intraocular pressure (IOP),fundus photography,the ONH parameters,and the RNFL thickness were taken every week before and after laser treatment.Results The main results were as follows:the IOP of the twelve eyes of the monkeys was 16.08±2.02 mmHg,the central corneal thickness was 489.17±17.82 μm and the ocular axials length was 20.32±0.84 mm.Eleven eyes,underwent 3 to 4 laser photocoagulation treatments,were induced to the COHT model.The average IOP in 27 weeks was 30.32±14.59 mmHg,and the variation was 8.19±7.45 mmHg.Optical coherence tomography displayed revealed the a lost rim area that changed from 1.67±0.44 mm2 to 0.43±0.34 mm2 in the monkey COHT model.The RNFL was changed from 97.92±6.79 μm to 64.46±17.44 μm.Conclusion The monkey COHT model mimicks the natural ocular hypertension/open angle glaucoma procedure and shares similar glaucomatous damage patterns,such as the reactivity of ONH to ocular hypertension,and the presence of the optic cup and the damage phenomenon of RNFL.The monkey model is an ideal animal model for studying damage of the optic nerve head and RNFL.
