中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2013年
30期
38-41
,共4页
T淋巴细胞,调节性%白细胞介素10%转化生长因子β1%激素抵抗性哮喘
T淋巴細胞,調節性%白細胞介素10%轉化生長因子β1%激素牴抗性哮喘
T림파세포,조절성%백세포개소10%전화생장인자β1%격소저항성효천
T-lymphocytes,regulatory%Interleukin-10%Transforming growth factor beta 1%Steroid-resistant asthma
目的 研究激素抵抗性哮喘(SRA)患者外周血CD4+ CD25+ Foxp3+调节性T细胞(Treg)及白细胞介素10(IL-10)、转化生长因子β1(TGF-β1)水平变化及其临床意义.方法 选取哮喘患者79例,分为SRA组(31例)和激素敏感性哮喘(SSA)组(48例).同时选取健康体检者45例作为对照组,采用流式细胞术检测CD4+ CD25+Foxp3+ Treg的表达水平,酶联免疫吸附法检测血清IL-10和TGF-β1水平.结果 SRA组和SSA组CD4+ CD25+ Foxp3+ Treg在CD4+T细胞中的比例和绝对值分别为0.0225±0.0063、(1.09±0.23)×107/L和0.0345±0.0094、(1.35±0.14)×107/L,均较对照组[0.0537±0.0128、(2.06±0.27)×107/L]明显下降,且SRA组的下降程度更加明显,差异均有统计学意义(P＜0.05).SRA组和SSA组血清TGF-β1水平均较对照组明显下降[(138.12±23.26)、(176.25±40.37) ng/L比(281.22±47.15) ng/L],差异有统计学意义(P＜0.05).SRA组血清IL-10水平较对照组明显下降[(516.43±86.33) ng/L比(763.02±90.19) ng/L],差异有统计学意义(P＜0.05).而SSA组血清IL-10水平与对照组比较差异无统计学意义(P＞ 0.05).SRA组血清IL-10和TGF-β1水平均较SSA组明显下降,差异有统计学意义(P＜0.05).SRA组和SSA组血清IL-10和TGF-β1水平与CD4+ CD25+ Foxp3+ Treg水平呈明显正相关(P＜0.01).结论 CD4+ CD25+ Foxp3+ Treg、IL-10和TGF-β1之间的相互作用在SRA的发生、发展中发挥了重要作用,而通过增加外周血CD4+ CD25+Foxp3+ Treg数量,稳定其功能,增加IL-10和TGF-β1表达,可能是治疗SRA的重要途径.
目的 研究激素牴抗性哮喘(SRA)患者外週血CD4+ CD25+ Foxp3+調節性T細胞(Treg)及白細胞介素10(IL-10)、轉化生長因子β1(TGF-β1)水平變化及其臨床意義.方法 選取哮喘患者79例,分為SRA組(31例)和激素敏感性哮喘(SSA)組(48例).同時選取健康體檢者45例作為對照組,採用流式細胞術檢測CD4+ CD25+Foxp3+ Treg的錶達水平,酶聯免疫吸附法檢測血清IL-10和TGF-β1水平.結果 SRA組和SSA組CD4+ CD25+ Foxp3+ Treg在CD4+T細胞中的比例和絕對值分彆為0.0225±0.0063、(1.09±0.23)×107/L和0.0345±0.0094、(1.35±0.14)×107/L,均較對照組[0.0537±0.0128、(2.06±0.27)×107/L]明顯下降,且SRA組的下降程度更加明顯,差異均有統計學意義(P＜0.05).SRA組和SSA組血清TGF-β1水平均較對照組明顯下降[(138.12±23.26)、(176.25±40.37) ng/L比(281.22±47.15) ng/L],差異有統計學意義(P＜0.05).SRA組血清IL-10水平較對照組明顯下降[(516.43±86.33) ng/L比(763.02±90.19) ng/L],差異有統計學意義(P＜0.05).而SSA組血清IL-10水平與對照組比較差異無統計學意義(P＞ 0.05).SRA組血清IL-10和TGF-β1水平均較SSA組明顯下降,差異有統計學意義(P＜0.05).SRA組和SSA組血清IL-10和TGF-β1水平與CD4+ CD25+ Foxp3+ Treg水平呈明顯正相關(P＜0.01).結論 CD4+ CD25+ Foxp3+ Treg、IL-10和TGF-β1之間的相互作用在SRA的髮生、髮展中髮揮瞭重要作用,而通過增加外週血CD4+ CD25+Foxp3+ Treg數量,穩定其功能,增加IL-10和TGF-β1錶達,可能是治療SRA的重要途徑.
목적 연구격소저항성효천(SRA)환자외주혈CD4+ CD25+ Foxp3+조절성T세포(Treg)급백세포개소10(IL-10)、전화생장인자β1(TGF-β1)수평변화급기림상의의.방법 선취효천환자79례,분위SRA조(31례)화격소민감성효천(SSA)조(48례).동시선취건강체검자45례작위대조조,채용류식세포술검측CD4+ CD25+Foxp3+ Treg적표체수평,매련면역흡부법검측혈청IL-10화TGF-β1수평.결과 SRA조화SSA조CD4+ CD25+ Foxp3+ Treg재CD4+T세포중적비례화절대치분별위0.0225±0.0063、(1.09±0.23)×107/L화0.0345±0.0094、(1.35±0.14)×107/L,균교대조조[0.0537±0.0128、(2.06±0.27)×107/L]명현하강,차SRA조적하강정도경가명현,차이균유통계학의의(P＜0.05).SRA조화SSA조혈청TGF-β1수평균교대조조명현하강[(138.12±23.26)、(176.25±40.37) ng/L비(281.22±47.15) ng/L],차이유통계학의의(P＜0.05).SRA조혈청IL-10수평교대조조명현하강[(516.43±86.33) ng/L비(763.02±90.19) ng/L],차이유통계학의의(P＜0.05).이SSA조혈청IL-10수평여대조조비교차이무통계학의의(P＞ 0.05).SRA조혈청IL-10화TGF-β1수평균교SSA조명현하강,차이유통계학의의(P＜0.05).SRA조화SSA조혈청IL-10화TGF-β1수평여CD4+ CD25+ Foxp3+ Treg수평정명현정상관(P＜0.01).결론 CD4+ CD25+ Foxp3+ Treg、IL-10화TGF-β1지간적상호작용재SRA적발생、발전중발휘료중요작용,이통과증가외주혈CD4+ CD25+Foxp3+ Treg수량,은정기공능,증가IL-10화TGF-β1표체,가능시치료SRA적중요도경.
Objective To study the clinical significance of CD4 + CD25+ Foxp3 + regulatory T cell (Treg) and interleukin 10 (IL-10),transforming growth factor-β1 (TGF-β 1) in patients with steroidresistant asthma (SRA).Methods Seventy-nine patients with asthma were divided into SRA group (31cases) and steroid-sensitive (SSA) group (48 cases).Forty-five healthy subjects were selected simultaneously as control group.CD4+ CD25+ Foxp3 +Treg level was detected by flow cytometry and serum IL-10 and TGF-β1 levels were detected by enzyme-linked immunosorbent assay.Results The percentage of CD4 + CD25 +Foxp3 +Treg in CD4+ T cell and its absolute value in SRA group and SSA group were 0.0225 ± 0.0063,(1.09 ± 0.23) × 107/L and 0.0345 ± 0.0094,(1.35 ± 0.14) × 107/L,they were significandy lower than those in control group [0.0537 ± 0.0128,(2.06 ± 0.27) × 107/L],and SRA group was significandy lower than SSA group,there were statistical differences (P ＜ 0.05).The levels of serum TGF-β1 in SRA group and SSA group were significantly lower than those in control group [(138.12 ± 23.26),(176.25 ± 40.37) ng/L vs.(281.22 ±47.15) ng/L],there was statistical difference (P ＜0.05).The levels of serum IL-10 in SRA group was significantly lower than that in control group [(516.43 ± 86.33)ng/L vs.(763.02 ± 90.19) ng/L],there was statistical difference (P ＜ 0.05).There was no statistical difference in the level of serum IL-10 between SSA group and control group (P ＞ 0.05).The levels of serum IL-10 and TGF-β 1 in SRA group were significantly lower than those in SSA group,there were statistical differences (P ＜ 0.05).The levels of serum IL-10 and TGF-β1 had positive correlation with CD4 + CD25 + Foxp3 +Treg in SRA group and SSA group (P ＜ 0.01).Conclusion The interaction among CD4+ CD25+ Foxp3 +Treg,IL-10 and TGF-β1 may play an important role in the SRA occurrence and development,while by increasing peripheral blood CD4 + CD25 + Foxp3 +Treg number and stabilizing its function can increase IL-10 and TGF-β1 expression,which may be an important way to treat SRA.
