目的 探讨NP方案加同期照射对人肺癌细胞增殖和凋亡的影响.方法 以A549、H1299为实验对象,设单纯照射组(A组)、酒石酸长春瑞滨+顺铂+同期照射组(B组),再按照照射剂量不同分为A-0、A-4、A-8组和B-0、B-4、B-8组.MTT方法用于检测酒石酸长春瑞滨+顺铂+同期照射对细胞增殖的影响,FACS用于检测酒石酸长春瑞滨+顺铂+同期照射对细胞凋亡的影响和细胞表面P-gp表达的影响.结果 除B-0组在48 h细胞抑制率与A-0组比较差异无统计学意义(P=0.103)外,B-0、B-4和B-8组在6,12,24和48 h时细胞抑制率均显著低于A-0、A-4和A-8组,差异均有统计学意义(P＜0.01).A-0组细胞凋亡率(4.01±0.95)％,A-4组为(20.19±3.76)％,A-8组为(45.34±4.77)％.A-8组细胞凋亡率显著高于A-4组,A-4组细胞凋亡率显著高于A-0组,差异均有统计学意义(t =7.17,P=0.006;t=7.23,P=0.019).B-0组细胞凋亡率(14.78±2.37)％,B-4组为(36.18±4.73)％,B-8组为(51.12±3.37)％.B-8组细胞凋亡率显著高于B-4组,B-4组细胞凋亡率显著高于B-0组,B-0组细胞凋亡率显著高于A-0组,B-4组细胞凋亡率显著高于A-4组,差异均有统计学意义(t=3.59,P=0.037;t=9.00,P=0.003;t=7.31,P=0.018;t=4.58,P=0.020),B-8组细胞凋亡率与A-8组比较差异无统计学意义(t=1.71,P=0.185).A-0组S周期细胞比例为(10.61±2.23)％,A-4组为(14.75±3.02)％,A-8组为(21.81±2.94)％;B-0组S周期细胞比例为(19.27±2.97)％,B-4组为(26.23±2.12)％,B-8组为(32.37±3.53)％.B-0、B-4和B-8组S周期细胞比例分别显著高于A-0、A-4和A-8组,差异均有统计学意义(t=4.04,P=0.027;t=5.35,P=0.013;t=3.98,P=0.028).A-0、A-4和A-8组P-gp荧光强度分别为(7.32±0.75)、(6.57±0.93)、(6.32±0.86) MFI,差异无统计学意义(P＞0.05).B-0、B-4和B-8组P-gp荧光强度分别为(5.12±0.81)、(3.76±0.74)、(2.16±0.24) MFI.B-0组P-gp荧光强度显著低于A-0组,B-4组P-gp荧光强度显著低于A-4组,B-8组P-gp荧光强度显著低于A-8组,差异均有统计学意义(t=-3.45,P=0.041;t=-5.85,P=0.010;t=-8.07,P=0.015).B-4组P-gp荧光强度显著低于B-0组,差异有统计学意义(t=-8.07,P=0.015),且与B-8组比较差异无统计学意义(t=-3.56,P=0.071).结论 NP方案加同期照射可以显著抑制人肺癌细胞增殖和凋亡,且降低肺癌细胞表面P-gp的表达.
目的 探討NP方案加同期照射對人肺癌細胞增殖和凋亡的影響.方法 以A549、H1299為實驗對象,設單純照射組(A組)、酒石痠長春瑞濱+順鉑+同期照射組(B組),再按照照射劑量不同分為A-0、A-4、A-8組和B-0、B-4、B-8組.MTT方法用于檢測酒石痠長春瑞濱+順鉑+同期照射對細胞增殖的影響,FACS用于檢測酒石痠長春瑞濱+順鉑+同期照射對細胞凋亡的影響和細胞錶麵P-gp錶達的影響.結果 除B-0組在48 h細胞抑製率與A-0組比較差異無統計學意義(P=0.103)外,B-0、B-4和B-8組在6,12,24和48 h時細胞抑製率均顯著低于A-0、A-4和A-8組,差異均有統計學意義(P＜0.01).A-0組細胞凋亡率(4.01±0.95)％,A-4組為(20.19±3.76)％,A-8組為(45.34±4.77)％.A-8組細胞凋亡率顯著高于A-4組,A-4組細胞凋亡率顯著高于A-0組,差異均有統計學意義(t =7.17,P=0.006;t=7.23,P=0.019).B-0組細胞凋亡率(14.78±2.37)％,B-4組為(36.18±4.73)％,B-8組為(51.12±3.37)％.B-8組細胞凋亡率顯著高于B-4組,B-4組細胞凋亡率顯著高于B-0組,B-0組細胞凋亡率顯著高于A-0組,B-4組細胞凋亡率顯著高于A-4組,差異均有統計學意義(t=3.59,P=0.037;t=9.00,P=0.003;t=7.31,P=0.018;t=4.58,P=0.020),B-8組細胞凋亡率與A-8組比較差異無統計學意義(t=1.71,P=0.185).A-0組S週期細胞比例為(10.61±2.23)％,A-4組為(14.75±3.02)％,A-8組為(21.81±2.94)％;B-0組S週期細胞比例為(19.27±2.97)％,B-4組為(26.23±2.12)％,B-8組為(32.37±3.53)％.B-0、B-4和B-8組S週期細胞比例分彆顯著高于A-0、A-4和A-8組,差異均有統計學意義(t=4.04,P=0.027;t=5.35,P=0.013;t=3.98,P=0.028).A-0、A-4和A-8組P-gp熒光彊度分彆為(7.32±0.75)、(6.57±0.93)、(6.32±0.86) MFI,差異無統計學意義(P＞0.05).B-0、B-4和B-8組P-gp熒光彊度分彆為(5.12±0.81)、(3.76±0.74)、(2.16±0.24) MFI.B-0組P-gp熒光彊度顯著低于A-0組,B-4組P-gp熒光彊度顯著低于A-4組,B-8組P-gp熒光彊度顯著低于A-8組,差異均有統計學意義(t=-3.45,P=0.041;t=-5.85,P=0.010;t=-8.07,P=0.015).B-4組P-gp熒光彊度顯著低于B-0組,差異有統計學意義(t=-8.07,P=0.015),且與B-8組比較差異無統計學意義(t=-3.56,P=0.071).結論 NP方案加同期照射可以顯著抑製人肺癌細胞增殖和凋亡,且降低肺癌細胞錶麵P-gp的錶達.
목적 탐토NP방안가동기조사대인폐암세포증식화조망적영향.방법 이A549、H1299위실험대상,설단순조사조(A조)、주석산장춘서빈+순박+동기조사조(B조),재안조조사제량불동분위A-0、A-4、A-8조화B-0、B-4、B-8조.MTT방법용우검측주석산장춘서빈+순박+동기조사대세포증식적영향,FACS용우검측주석산장춘서빈+순박+동기조사대세포조망적영향화세포표면P-gp표체적영향.결과 제B-0조재48 h세포억제솔여A-0조비교차이무통계학의의(P=0.103)외,B-0、B-4화B-8조재6,12,24화48 h시세포억제솔균현저저우A-0、A-4화A-8조,차이균유통계학의의(P＜0.01).A-0조세포조망솔(4.01±0.95)％,A-4조위(20.19±3.76)％,A-8조위(45.34±4.77)％.A-8조세포조망솔현저고우A-4조,A-4조세포조망솔현저고우A-0조,차이균유통계학의의(t =7.17,P=0.006;t=7.23,P=0.019).B-0조세포조망솔(14.78±2.37)％,B-4조위(36.18±4.73)％,B-8조위(51.12±3.37)％.B-8조세포조망솔현저고우B-4조,B-4조세포조망솔현저고우B-0조,B-0조세포조망솔현저고우A-0조,B-4조세포조망솔현저고우A-4조,차이균유통계학의의(t=3.59,P=0.037;t=9.00,P=0.003;t=7.31,P=0.018;t=4.58,P=0.020),B-8조세포조망솔여A-8조비교차이무통계학의의(t=1.71,P=0.185).A-0조S주기세포비례위(10.61±2.23)％,A-4조위(14.75±3.02)％,A-8조위(21.81±2.94)％;B-0조S주기세포비례위(19.27±2.97)％,B-4조위(26.23±2.12)％,B-8조위(32.37±3.53)％.B-0、B-4화B-8조S주기세포비례분별현저고우A-0、A-4화A-8조,차이균유통계학의의(t=4.04,P=0.027;t=5.35,P=0.013;t=3.98,P=0.028).A-0、A-4화A-8조P-gp형광강도분별위(7.32±0.75)、(6.57±0.93)、(6.32±0.86) MFI,차이무통계학의의(P＞0.05).B-0、B-4화B-8조P-gp형광강도분별위(5.12±0.81)、(3.76±0.74)、(2.16±0.24) MFI.B-0조P-gp형광강도현저저우A-0조,B-4조P-gp형광강도현저저우A-4조,B-8조P-gp형광강도현저저우A-8조,차이균유통계학의의(t=-3.45,P=0.041;t=-5.85,P=0.010;t=-8.07,P=0.015).B-4조P-gp형광강도현저저우B-0조,차이유통계학의의(t=-8.07,P=0.015),차여B-8조비교차이무통계학의의(t=-3.56,P=0.071).결론 NP방안가동기조사가이현저억제인폐암세포증식화조망,차강저폐암세포표면P-gp적표체.
Objective To study the effect of NP regimen plus concurrent irradiation on cancer cell survival of human lung adenocarcinoma.Methods A549 and H1299 cells were served as the experimental objects and they were divided into irradiation group (A group),and vinorelbine tartrate injection plus cisplatin with concurrent radiotherapy group (B group).According to the different irradiation doses A group and B group were divided into A-0,A-4,A-8 group and B-0,B-4,B-8 group.MTT was performed to measure the effect of NP regimen plus concurrent irradiation on the cell proliferation,and FACS was used to detect the effect of NP regimen plus concurrent irradiation on apoptosis and P-gp expression.Results Except the cell inhibition rate of B-0 group and A-0 group had no significant difference,the cell inhibition rate of B-0,B-4 and B-8 group at 6,12,24 and 48 h were significantly lower than those of A-0,A-4 and A-8 group.The apoptosis rate of A-8 group [(45.34 ±4.77)％] was significantly higher than that of A-4 group [(20.19 ± 3.76)％] (t =7.17,P =0.006),the apoptosis rate of A-4 group was significantly higher than that of A-0 group [(4.01 ± 0.95)％] (t =7.23,P=0.019).The apoptosis rate ofB-8 group [(51.12 ± 3.37)％]was significantly higher than that of B-4 group[(36.18 ± 4.73)％] (t =3.59,P =0.037),the apoptosis rate of B-4 group was significantly higher than that of B-0 group [(14.78 ± 2.37)％] (t =9.00,P =0.003).The apoptosis rate of B-0 group [(14.78 ± 2.37)％] was significantly higher than that of A-0 group [(4.01±0.95)％] (t =7.31,P =0.018),the apoptosis rate of B-4 group was significantly higher than that of A-4 group (t =4.58,P=0.020),but there was no significant difference between B-8 group and A-8 group (t =1.71,P =0.185).The S cycle percentage of cells in B-0,B-4 and B-8 groups were significantly higher than those of A-0,A-4 and A-8 group (P ＜0.05).There were no significant differences among the P-gp fluorescence intensity of A-0,A-4 and A-8 group (P ＞ 0.05).The P-gp fluorescence intensity of B-0 group was significantly lower than that of A-0 group (t =-3.45,P =0.041),the P-tp fluorescence intensity of B-4 group was significantly lower than that of A-4 group(t =-5.85,P =0.010).The P-gp fluorescence intensity of B-8 group was significantly lower than that of A-8 group (t =-8.07,P =0.015).The P-gp fluorescence intensity of B-4 group was significantly lower than that of B-0 group (t =-8.07,P=0.015),and there was no significant difference between the mean fluorescence intensity of B-4 group and B-8 group (t =-3.56,P =0.071).Conclusion NP regimen plus concurrent irradiation could significantly inhibit the proliferation and apoptosis of human lung cancer cells,and decrease the expression of P-gp in lung cancer cells.
